We narrowed to 38 results for: clu

1. AAV Production in HEK293 Cells

   Type

   Protocol

   ...multiple times to obtain a single cell suspension (no clumps of cells). Pool cells from 2 x T-175 flasks. Adjust...vigorously to obtain a single cell suspension (no clumps). Count cells using a hemocytometer or cell counter...

2. CRISPR Library Amplification

   Type

   Protocol

   ...of Endura Duos, Lucigen, 60242-1) Alternatives include Stbl4 cells or other ultra-high efficiency electrocompetent..., gentler practices, and colder reagents (not including the recovery media!). Do not proceed with Maxipreps...

3. Protocol - pLKO.1 – TRC Cloning Vector

   Type

   Protocol

   ...efficiently infect a broad range of cell types, including both dividing and non-dividing cells. Addition...warranty of any kind either expressed or implied, including but not limited to the implied warranties of fitness...

4. Plasmid Modification by Annealed Oligo Cloning (with Protocols)

   Type

   Protocol

   ...AATTAATT CCGCGCGG GTTAAC - 5' = 28 bp We also need to include additional bases to complement the overhangs generated...

5. Protocol - How to Perform a Diagnostic Digest

   Type

   Protocol

   ...that cut your plasmid. Many DNA analysis tools, including Addgene’s Sequence Analyzer , allow you to identify...

6. Centrifugation

   Type

   Protocol

   ...to save the pellet, the supernatant, or both. Conclusion Centrifuges are important tools for many different...

7. Molecular Biology Protocol - Restriction Digest of Plasmid DNA

   Type

   Protocol

   ...Activity" and can happen for a variety of reasons, including high glycerol concentration. Learn more at (Link...

8. General Transfection

   Type

   Protocol

   ...transfection; therefore, plasmid DNA purification should include an endotoxin removal step. For high quality plasmid...

9. Protocol - Bacterial Transformation

   Type

   Protocol

   ...should also add a positive control (many companies include a positive control plasmid with their competent...

10. Lentivirus Production

    Type

    Protocol

    ...transfection; therefore, plasmid DNA purification should include an endotoxin removal step. For high quality plasmid...

11. Colony Formation Titering Assay

    Type

    Protocol

    ...to each well (each well gets one dilution) Also include an untransduced well by using 150 μL of media in...

12. Using a Light Microscope Protocol

    Type

    Protocol

    ...others can interpret your images in the future. Conclusion Like any important laboratory instrument, you...

13. Pipetting Protocol

    Type

    Protocol

    ... discard into a biohazardous waste container. Conclusion Pipetting accurately and precisely is important...

14. Transfection for Recombinant Antibodies

    Type

    Protocol

    ... or recommend specific products or equipment. Inclusion of this information is solely for transparency...

15. AAV Purification by Iodixanol Gradient Ultracentrifugation

    Type

    Protocol

    ... to remove contaminants with lower densities, including empty capsids. The 60% step serves as a cushion...

16. Pouring LB Agar Plates

    Type

    Protocol

    ...with the date and the medium they will contain including the identity of the antibiotic. Pro-Tip We batch...

17. Plasmid Cloning by PCR (with Protocols)

    Type

    Protocol

    ...similarly, taking the final 18bases of the ORF, including the stop codon (5'-TGGCATATCTCGAAGTACTGA-3'), ...

18. Coomassie Purity Stain of Recombinant Antibodies

    Type

    Protocol

    ... or recommend specific products or equipment. Inclusion of this information is solely for transparency...