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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 44 results for: lars;

Showing: 21 - 40 of 44 results

  1. Protocol - Bacterial Transformation

    Type
    Protocol
    ...less efficient at taking up larger plasmids. If you need to transform large plasmids, it is a good idea...very few if any transformants when transforming large plasmids (>10 kb) or BACs, what can I do? Chemically...

  2. Pipetting Protocol

    Type
    Protocol
    ...pipette is an essential tool for nearly anyone in molecular biology. You’ll use a pipette in many scenarios... liquid. Pro-Tip If pipetting from a relatively large container holding a small volume of liquid, tilt... of and gently in contact with the container (capillary action between the container and the liquid will...

  3. Transfection for Recombinant Antibodies

    Type
    Protocol
    ...Antibody Plasmid Collection Antibodies Blog Posts Molecular Biology Reference General Transfection Protocol...and reagents from other vendors should produce similar outcomes when using these protocols. However, please...replication use a HEK293 line stably expressing the SV40 large T antigen. For antibody-expressing plasmids containing...

  4. AAV Purification by Iodixanol Gradient Ultracentrifugation

    Type
    Protocol
    ... an intravenous isomolar contrast agent. In research, iodixanol is used as an isomolar density gradient...60% iodixanol step Carefully add up to 5 mL of clarified supernatant on top of the gradient. Use 1X PBS...

  5. Kit Free RNA Extraction

    Type
    Protocol
    ...Kit-Free DNA Purification Agarose Gel Purification Molecular Biology Reference Introduction This protocol describes... (Sarkosyl) 0.1 M 2-mercaptoethanol TRIzol® or similar product such as TRI Reagent®, RNAzol®, QIAzol® ...Homogenize or lyse tissues or cells in TRIzol® or a similar product. For tissues: use 1 mL of TRIzol® per 100...

  6. CRISPR Library Amplification

    Type
    Protocol
    ... Pooled Libraries All CRISPR Pooled Libraries Molecular Biology Reference How do I process my Addgene ...libraries up to 200,000 individual plasmids. For larger libraries scale the number of cells and DNA accordingly... LB Agar + Antibiotic 245 mm bioassay plates (Molecular Devices, X602) Pro-Tip Pour these plates at least...

  7. DNA Quantification

    Type
    Protocol
    ...Information During several different stages of molecular cloning, it is important to get a quick and accurate... a liquid to determine the concentration of a particular substance in that liquid. Molecules absorb different...

  8. Protocol - How to Design Primers

    Type
    Protocol
    ...also use a nucleotide-based molecular insert (inosine) instead of a regular nucleotide for broader pairing...

  9. Protocol - How to Streak a Plate

    Type
    Protocol
    ... plate, do NOT dig into the agar. Another very popular technique is to draw in discontinuous lines. Start...30 °C instead of 37 °C. This is often true for large unstable plasmids, which sometimes recombine at ...

  11. Ligation Independent Cloning

    Type
    Protocol
    ... like... Cloning Methods Empty Backbone Guide Molecular Biology Protocols Overview Ligation Independent...Transform Mix your treated vector and insert at a molar ratio of 1:2 or 1:3, using between 20 and 50 ng ...

  12. Video Library

    Type
    Protocol
    ...success in numerous fields such as microbiology, molecular biology or cell biology. Learn some of the key...samples. This is a commonly used technique for molecular cloning, such as PCR- or restriction enzyme-based...

  13. Handling Plasmids from Addgene - Purifying Plasmid DNA

    Type
    Protocol
    ... a Liquid Bacterial Culture Introduction Many molecular biology techniques require highly purified and... of bacteria to grow (low copy plasmids require larger cultures). Centrifuge the culture to pellet the...

  15. AAV Titration by qPCR Using SYBR Green Technology

    Type
    Protocol
    ...from academic viral vector cores. Our titers were similar to those reported by these institutions. This protocol...Concentration of Addgene plasmid #59462 : 1.07 μg/μL Molecular Weight: 6208 bp x 650 dalton/bp or (g/mole)/bp...

  16. Antibody Validation Using the Indirect ELISA Method

    Type
    Protocol
    ...and reagents from other vendors should produce similar outcomes when using these protocols. However, please...plate washer (optional) Scale Microsoft Excel or similar software Reagents Purified antigen Purified recombinant...

  17. Isolating a Monoclonal Cell Population by Limiting Dilution

    Type
    Protocol
    ...empirically, and that the cell population be monitored regularly for transgene expression. This protocol has been...over-confluent, trypsinize the cells and expand them to larger culture dishes, being sure not to cross contaminate...

  18. Protocol - pLKO.1 – TRC Cloning Vector

    Type
    Protocol
    ...37°C, 5% CO 2 overnight. Although cells should regularly be passaged in DMEM + 10% FBS with penicillin/...limited to the implied warranties of fitness for a particular purpose, non-infringement, typicality, safety...

  19. Weighing Reagents Protocol

    Type
    Protocol
    ...places. Pro-Tip If you’re weighing out an amount larger than the capacity of your balance or weighing boat...

  20. Fluorescence Titering Assay

    Type
    Protocol
    ...cells for mycoplasma (Link opens in a new window) regularly Do not over- or under-grow your cells. Thaw a ...
Showing: 21 - 40 of 44 results