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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 41 results for: omp

Showing: 21 - 40 of 41 results

  1. AAV ddPCR Titration

    Type
    Protocol
    ...layout is complete, select 'Run' to begin the droplet reading. When the droplet reading is complete, export... on the insert. Please ensure that you are in compliance with your institution’s biosafety regulations...Stabilization 98 10 2 1 Hold 4 ∞ 2 1 After PCR is complete, transfer the plate to the Droplet Reader. Open...

  2. Ligation Independent Cloning

    Type
    Protocol
    ...restriction digest protocol ). After the digest is complete, you will need to separate the linearized vector... A typical T4 Pol reaction is shown. Mix the components (add the T4 last) and incubate at room temperature...of T4 Pol in the following step. This may be accomplished by gel purification . Step 5: Create Insert ...

  3. Centrifugation

    Type
    Protocol
    ... and using a centrifuge to separate different components in a liquid sample....laboratory equipment used to separate different components in a liquid sample. It achieves this by using... you use, most will have essentially the same components. The photo to the left shows a front view of ...

  4. Gibson Assembly Protocol

    Type
    Protocol
    ...2009). Regardless of fragment length or end compatibility, multiple overlapping DNA fragments can be joined...overhangs by chewing back from the DNA 5’ end. Complementary DNA fragments can subsequently anneal to each... DNA Ligase - covalently joins the annealed complementary DNA fragments, removing any nicks and creating...

  5. Protocol - How to Create a Bacterial Glycerol Stock

    Type
    Protocol
    ...bacterial strain and you will not need to obtain more competent cells and retransform. Bacteria on an LB agar ...streaking onto LB agar will prevent it from thawing completely and will improve the shelf life. It is very important...

  7. Transfection for Recombinant Antibodies

    Type
    Protocol
    ...biosafety level 2. Please ensure that you are in compliance with your institution’s biosafety regulations...tips Pipettor and pipettes Benchtop centrifuge compatible with 50 mL conical tubes Automated cell counter...

  8. AAV Purification by Iodixanol Gradient Ultracentrifugation

    Type
    Protocol
    ...vivo . The iodixanol gradient in this protocol is composed of steps that separate out contaminants from an...exchange and concentration Note: Both steps could be completed in one (long) day. Equipment Ultracentrifuge T70i...

  9. Protocol - How to Run an Agarose Gel

    Type
    Protocol
    ...). Microwave for 1-3 min until the agarose is completely dissolved (but do not overboil the solution, ...room temperature for 20-30 mins, until it has completely solidified. Pro-Tip If you are in a hurry, the...

  10. Handling Plasmids from Addgene - Purifying Plasmid DNA

    Type
    Protocol
    ...bacterial cultures , please see those pages. Several companies, such as (Link opens in a new window) NEB , (Link...but within a smaller volume of buffer that is compatible with the next solution. Add a denaturing solution...

  11. Antibody Validation Using the Indirect ELISA Method

    Type
    Protocol
    ...science. General Considerations The ELISA can be completed in a single day or broken up over several days...ELISA plate washer. Equipment Spectrophotometer compatible with 96-well plates 1–10 µL single channel pipette...

  12. Kit Free RNA Extraction

    Type
    Protocol
    ...to allow for dissociation of the nucleoprotein complexes. The effectiveness of your RNA isolation will ...

  13. Protocol - How to Streak a Plate

    Type
    Protocol
    ...white dot growing on the solid medium. This dot is composed of millions of genetically identical bacteria ...

  16. Weighing Reagents Protocol

    Type
    Protocol
    ...reagents is important to ensure the accurate composition of your solutions and buffers. Now that you have...

  18. Video Library

    Type
    Protocol
    ...positions he has held in his career, his favorite company tradition, and Addgene's role in the scientific...

  19. Immunocytochemistry

    Type
    Protocol
    ...concentrations, incubation times, and recommended compatible reagents. Secondary antibodies must match the...

  20. Pouring LB Agar Plates

    Type
    Protocol
    ...autoclave. Pro-Tip Once your autoclave cycle is complete, we recommend opening the door to the autoclave...
Showing: 21 - 40 of 41 results