...1 1 μL AgeI add ddH 2 O to bring to 50 μL final volume Incubate at 37°C for 2 hours. Purify with Qiaquick... DNA ligase add ddH 2 O to bring to 20 μL final volume Incubate at 16°C for 4-20 hours. Transform 2 μL...0.8 μL NcoI add ddH 2 O to bring to 20 μL final volume Incubate at 37°C for 1-2 hours. 4. Run the digestion...master mix to each tube from step c for a total volume of 100 μL. Pipette master mix directly into the...

...loosely cover the entire top of the bottle with aluminum foil. Autoclave and allow to cool to room temperature... swirl. Loosely cover the culture with sterile aluminum foil or a cap that is not air tight. Incubate ...

...Inactivate T4 Pol by heating to 75° for 20 minutes. Volume (μl) Reagent Final Conc. 4 10X Buffer 1X 20-30 ...annealing reaction. This should be done in a small volume with no additional water (<5 μl). It is advisable...

...often an extra tube filled with water to the same volume as your samples. If your samples are significantly...need to fill the blank based on weight instead of volume. *Pro-Tip* For microfuge tubes, place them with...

...with videos for select protocols in the right-hand column. You can find even more video content on the Addgene...recombinant antibodies using Protein A or Protein G columns Watch the Video! Coomassie Purity Stain Determine...

...recover all transformants. *Pro-Tip* If the culture volume is too big, gently collect the cells by centrifugation...centrifugation and resuspend in a smaller volume of LB so that there isn't too much liquid media on the agar...

...of HCl at a time. Add deionized water to a final volume of 1 L and recheck pH to ensure that it has not...up to 4 times for a total of 16% of the culture volume. At 72-96 h post-transfection, supplement the flask...

... . Simply adjust the mass of agarose in a given volume to make gels of other agarose concentrations (e.g...sample you want to load on a gel, make 10% more volume than needed because several microliters can be ...

...to a microfuge tube. Add 8 µL of PBS to a total volume of 13 µL . Prepare the recombinant antibody Dilute... issues. Plot the mg/mL versus the HC + LC Area column for the IgG standards in Google Sheets. Open exported...

...purity. Note: Purity is measured under the 260/280 column (A good purity ranges from 1.80-2.00). Repeat for...

... mostly absorbed (there is a very small visible volume of pooled liquid remaining on the surface). We ...

...concentrations. We recommend mixing 2μg each in a total volume of 50μL - add additional annealing buffer if necessary...

... of reagent that will be resuspended in a small volume (e.g. 0.02 g to resuspend in 1 mL), you may place...

...and vector to use for the ligation reaction. The volume of vector DNA and insert DNA used in the ligation...

...Transfer Plasmid* 1.64 pmol OptiPro SFM to total volume 500 μL *Plasmid concentrations and ratios should...

...sample. To ensure that your sample is adequately illuminated, look through the eyepiece. Adjust the power ...