Showing: 81 - 100 of 12622 results
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Plasmid#44758DepositorInsertHuman codon optimized Cas9
UseCRISPRTags32 amino acid linker, Flag, and NLSExpressionMammalianMutationcodon-optimizedPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pHR-SFFV-dCas9-BFP
Plasmid#46910PurposeHuman expression vector containing SFFV promoter, dCas9 that is fused to 2x NLS and tagBFPDepositorInsertdCas9-BFP fusion
UseCRISPR and LentiviralTags2xNLS, BFP, and HAExpressionMammalianMutationPromoterSFFVAvailabilityAcademic Institutions and Nonprofits only -
pHR-SFFV-dCas9-BFP-KRAB
Plasmid#46911PurposeHuman expression vector containing SFFV promoter, dCas9 that is fused to 2x NLS, tagBFP and a KRAB domainDepositorInsertdCas9-BFP-KRAB fusion
UseCRISPR and LentiviralTags2xNLS, BFP, HA, and KRAB domainExpressionMammalianMutationPromoterSFFVAvailabilityAcademic Institutions and Nonprofits only -
pMSCV-LTR-dCas9-VP64-BFP
Plasmid#46912PurposeHuman expression vector containing MSCV LTR promoter, dCas9 that is fused to 2x NLS, VP64 and tagBFPDepositorInsertsdCas9-VP64-BFP fusion
Puromycin resistance
UseCRISPR and RetroviralTags3xNLS, BFP, and VP64 domainExpressionMammalianMutationPromoterLTR and PGKAvailabilityAcademic Institutions and Nonprofits only -
pMSCV-LTR-dCas9-p65AD-BFP
Plasmid#46913PurposeHuman expression vector containing MSCV LTR promoter, dCas9 that is fused to 2x NLS, p65 activation domain and tagBFPDepositorInsertsdCas9-p65AD-BFP fusion
Puromycin resistance
UseCRISPR and RetroviralTags2xNLS, BFP, and VP64 domainExpressionMammalianMutationPromoterLTR and PGKAvailabilityAcademic Institutions and Nonprofits only -
pU6-sgGFP-NT1
Plasmid#46914PurposeHuman pSico-based U6 vector containing murine U6 promoter and sgRNA targeting GFP (NT1)DepositorInsertsgGFP-NT1
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6AvailabilityAcademic Institutions and Nonprofits only -
pU6-sgGAL4-1
Plasmid#46915PurposeHuman pSico-based U6 vector containing murine U6 promoter and sgRNA targeting GAL4 UAS promoterDepositorInsertssgGAL4-1
Puromycin resistance and mCherry
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterCMV and U6AvailabilityAcademic Institutions and Nonprofits only -
pU6-sgGAL4-4
Plasmid#46916PurposeHuman pSico-based U6 vector containing murine U6 promoter and sgRNA targeting GAL4 UAS promoter (negative control)DepositorInsertssgGAL4-4
Puromycin resistance and mCherry
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterCMV and U6AvailabilityAcademic Institutions and Nonprofits only -
pU6-sgCXCR4-2
Plasmid#46917PurposeHuman pSico-based U6 vector containing murine U6 promoter and sgRNA targeting endogenous CXCR4 geneDepositorInsertsUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterCMV and U6AvailabilityAcademic Institutions and Nonprofits only -
pU6-sgCD71-2
Plasmid#46918PurposeHuman pSico-based U6 vector containing murine U6 promoter and sgRNA targeting endogenous CD71 geneDepositorInsertsUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterCMV and U6AvailabilityAcademic Institutions and Nonprofits only -
pMLS-SV40-EGFP
Plasmid#46919PurposeTarget EGFP gene that is stably integrated into HEK293 genomeDepositorInsertGFP
UseCRISPR and RetroviralTagsExpressionMammalianMutationPromoterSV40AvailabilityAcademic Institutions and Nonprofits only -
pTDH3-dCas9
Plasmid#46920PurposeYeast CEN/ARS vector (Leu2) that contains dCas9 fused to NLS controlled by TDH3 promoterDepositorInsertdCas9
UseCRISPRTagsExpressionYeastMutationPromoterTDH3AvailabilityAcademic Institutions and Nonprofits only -
pTDH3-dCas9-Mxi1
Plasmid#46921PurposeYeast CEN/ARS vector (Leu2) that contains dCas9 fused to NLS and Mxi1 domain controlled by TDH3 promoterDepositorInsertdCas9-Mxi1
UseCRISPRTagsExpressionYeastMutationPromoterTDH3AvailabilityAcademic Institutions and Nonprofits only -
pSNR52-sgTEF1
Plasmid#46922PurposeYeast CEN/ARS vector (Ura3) that contains sgRNA controlled by SNR 52 promoter, targeting endogenous TEF1 promoterDepositorInsertsgTEF1 promoter (TEF1 Budding Yeast)
UseCRISPRTagsExpressionYeastMutationPromoterSNR52AvailabilityAcademic Institutions and Nonprofits only -
pSNR52-sgTET
Plasmid#46923PurposeYeast CEN/ARS vector (Ura3) that contains sgRNA controlled by SNR 52 promoter, targeting endogenous TRE elements of pTET07 promoterDepositorInsertsgRNA targeting endogenous TRE elements of pTET07 promoter
UseCRISPRTagsExpressionYeastMutationPromoterSNR52AvailabilityAcademic Institutions and Nonprofits only -
pK7WGF2::hCas9
Plasmid#46965PurposeExpesses the human codon usage Cas9 nuclease (Mali et al. Science 339, 823-826, 2013) with an N-terminal GFP tag from the 35S promoter in the plant tissueDepositorInserthCas9
UseCRISPR; Plant expressionTagseGFPExpressionMutationPromoter35SAvailabilityAcademic Institutions and Nonprofits only -
pICH86966::AtU6p::sgRNA_PDS
Plasmid#46966PurposeExpresses an sgRNA targeting the PDS gene in Nicotiana benthamiana from the Arabidopsis U6 promoterDepositorInsertAtU6p::sgRNA_PDS
UseCRISPR; Plant expressionTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pICSL01009::AtU6p
Plasmid#46968PurposeEncodes the Arabidopsis U6 promoter in a level 0 vector (Weber et al. PLoS One 6:e16765, 2011). It is used to place an sgRNA uder the Arabidopsis U6 promoter.DepositorInsertAtU6p
UseCRISPRTagsExpressionPlantMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pUC gRNA Shuttle
Plasmid#47024PurposeEncodes a template from which gRNAs can be made via InFusion cloning. The Medicago truncatula U6.6 promoter drives the gRNA. For use in plants.DepositorInsertgRNA Shuttle
UseCRISPR; Cas9TagsExpressionPlantMutationG to T cloning mutation at position 323PromoterMedicago truncatula U6.6AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-dCas9
Plasmid#47106PurposeExpresses inactivated S. pyogenes dCas9 (D10A, H840A) in mammalian cellsDepositorInsertdCas9
UseCRISPRTagsFlag and SV40 NLSExpressionMammalianMutationD10A, H840A (catalytically inactive)PromoterCMVAvailabilityAcademic Institutions and Nonprofits only
Showing: 81 - 100 of 12622 results