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pCMV-PE2-P2A-GFP Citations (20)

Originally described in: Search-and-replace genome editing without double-strand breaks or donor DNA.
Anzalone AV, Randolph PB, Davis JR, Sousa AA, Koblan LW, Levy JM, Chen PJ, Wilson C, Newby GA, Raguram A, Liu DR Nature. 2019 Oct 21. pii: 10.1038/s41586-019-1711-4. doi: 10.1038/s41586-019-1711-4.
PubMed Journal

Articles Citing pCMV-PE2-P2A-GFP

Articles
Precise correction of Duchenne muscular dystrophy exon deletion mutations by base and prime editing. Chemello F, Chai AC, Li H, Rodriguez-Caycedo C, Sanchez-Ortiz E, Atmanli A, Mireault AA, Liu N, Bassel-Duby R, Olson EN. Sci Adv. 2021 Apr 30;7(18). pii: 7/18/eabg4910. doi: 10.1126/sciadv.abg4910. Print 2021 Apr. PubMed
Evaluating CRISPR-based prime editing for cancer modeling and CFTR repair in organoids. Geurts MH, de Poel E, Pleguezuelos-Manzano C, Oka R, Carrillo L, Andersson-Rolf A, Boretto M, Brunsveld JE, van Boxtel R, Beekman JM, Clevers H. Life Sci Alliance. 2021 Aug 9;4(10). pii: 4/10/e202000940. doi: 10.26508/lsa.202000940. Print 2021 Oct. PubMed
Lupus enhancer risk variant causes dysregulation of IRF8 through cooperative lncRNA and DNA methylation machinery. Zhou T, Zhu X, Ye Z, Wang YF, Yao C, Xu N, Zhou M, Ma J, Qin Y, Shen Y, Tang Y, Yin Z, Xu H, Zhang Y, Zang X, Ding H, Yang W, Guo Y, Harley JB, Namjou B, Kaufman KM, Kottyan LC, Weirauch MT, Hou G, Shen N. Nat Commun. 2022 Apr 6;13(1):1855. doi: 10.1038/s41467-022-29514-y. PubMed
A time-resolved, multi-symbol molecular recorder via sequential genome editing. Choi J, Chen W, Minkina A, Chardon FM, Suiter CC, Regalado SG, Domcke S, Hamazaki N, Lee C, Martin B, Daza RM, Shendure J. Nature. 2022 Jul 6. pii: 10.1038/s41586-022-04922-8. doi: 10.1038/s41586-022-04922-8. PubMed

Associated Plasmids

ATF4-dependent fructolysis fuels growth of glioblastoma multiforme. Chen C, Zhang Z, Liu C, Wang B, Liu P, Fang S, Yang F, You Y, Li X. Nat Commun. 2022 Oct 16;13(1):6108. doi: 10.1038/s41467-022-33859-9. PubMed
Precise genomic editing of pathogenic mutations in RBM20 rescues dilated cardiomyopathy. Nishiyama T, Zhang Y, Cui M, Li H, Sanchez-Ortiz E, McAnally JR, Tan W, Kim J, Chen K, Xu L, Bassel-Duby R, Olson EN. Sci Transl Med. 2022 Nov 23;14(672):eade1633. doi: 10.1126/scitranslmed.ade1633. Epub 2022 Nov 23. PubMed
Challenges in Gene Therapy for Somatic Reverted Mosaicism in X-Linked Combined Immunodeficiency by CRISPR/Cas9 and Prime Editing. Hou Y, Urena-Bailen G, Mohammadian Gol T, Gratz PG, Gratz HP, Roig-Merino A, Antony JS, Lamsfus-Calle A, Daniel-Moreno A, Handgretinger R, Mezger M. Genes (Basel). 2022 Dec 13;13(12):2348. doi: 10.3390/genes13122348. PubMed
Prediction of prime editing insertion efficiencies using sequence features and DNA repair determinants. Koeppel J, Weller J, Peets EM, Pallaseni A, Kuzmin I, Raudvere U, Peterson H, Liberante FG, Parts L. Nat Biotechnol. 2023 Feb 16. doi: 10.1038/s41587-023-01678-y. PubMed
Parallel engineering and activity profiling of a base editor system. Fong JHC, Chu HY, Zhou P, Wong ASL. Cell Syst. 2023 May 17;14(5):392-403.e4. doi: 10.1016/j.cels.2023.03.007. Epub 2023 May 9. PubMed
Increased prime edit rates in KCNQ2 and SCN1A via single nicking all-in-one plasmids. Dirkx N, Weuring WJ, De Vriendt E, Smal N, van de Vondervoort J, van 't Slot R, Koetsier M, Zonnekein N, De Pooter T, Weckhuysen S, Koeleman BPC. BMC Biol. 2023 Jul 13;21(1):156. doi: 10.1186/s12915-023-01646-7. PubMed

Associated Plasmids

Bioluminescence Assay of Lysine Deacylase Sirtuin Activity. Van Scoyk AN, Antelope O, Franzini A, Ayer DE, Peterson RT, Pomicter AD, Owen SC, Deininger MW. bioRxiv [Preprint]. 2023 Aug 14:2023.08.10.552871. doi: 10.1101/2023.08.10.552871. PubMed
Flow cytometry-based quantification of genome editing efficiency in human cell lines using the L1CAM gene. Hasan MN, Hyodo T, Biswas M, Rahman ML, Mihara Y, Karnan S, Ota A, Tsuzuki S, Hosokawa Y, Konishi H. PLoS One. 2023 Nov 9;18(11):e0294146. doi: 10.1371/journal.pone.0294146. eCollection 2023. PubMed
Benzomorphan and non-benzomorphan agonists differentially alter sigma-1 receptor quaternary structure, as does types of cellular stress. Couly S, Yasui Y, Foncham S, Grammatikakis I, Lal A, Shi L, Su TP. Cell Mol Life Sci. 2024 Jan 9;81(1):14. doi: 10.1007/s00018-023-05023-z. PubMed
iSCORE-PD: an isogenic stem cell collection to research Parkinson's Disease. Busquets O, Li H, Mohieddin Syed K, Jerez PA, Dunnack J, Bu RL, Verma Y, Pangilinan GR, Martin A, Straub J, Du Y, Simon VM, Poser S, Bush Z, Diaz J, Sahagun A, Gao J, Hernandez DG, Levine KS, Booth EO, Bateup HS, Rio DC, Hockemeyer D, Blauwendraat C, Soldner F. bioRxiv [Preprint]. 2024 Feb 13:2024.02.12.579917. doi: 10.1101/2024.02.12.579917. PubMed
Prime editing-mediated correction of the leptin receptor in muscle cells of db/db mice. Lee KE, Xu Y, Geng B, Kim J, Kellon N, He Z, Zhang Z, Li D, Gouchoe DA, Zhu H. Biotechnol J. 2024 May;19(5):e2300676. doi: 10.1002/biot.202300676. PubMed
ISGylation enhances dsRNA-induced interferon response and NFkappaB signaling in fallopian tube epithelial cells. Madaan V, Kollara A, Spaner D, Brown TJ. J Biol Chem. 2024 Sep;300(9):107686. doi: 10.1016/j.jbc.2024.107686. Epub 2024 Aug 17. PubMed
Engineering oncogenic hotspot mutations on SF3B1 via CRISPR-directed PRECIS mutagenesis. Fernandez M, Yu L, Jia Q, Wang X, Hart KL, Jia Z, Lin RJ, Wang L. Cancer Res Commun. 2024 Aug 28. doi: 10.1158/2767-9764.CRC-24-0145. PubMed

Associated Plasmids

Modeling the atrioventricular conduction axis using human pluripotent stem cell-derived cardiac assembloids. Li J, Wiesinger A, Fokkert L, Bakker P, de Vries DK, Tijsen AJ, Pinto YM, Verkerk AO, Christoffels VM, Boink GJJ, Devalla HD. Cell Stem Cell. 2024 Nov 7;31(11):1667-1684.e6. doi: 10.1016/j.stem.2024.08.008. Epub 2024 Sep 10. PubMed
Bioluminescence assay of lysine deacylase sirtuin activity. Van Scoyk AN, Antelope O, Ayer DE, Peterson RT, Pomicter AD, Owen SC, Deininger MW. Cell Chem Biol. 2024 Nov 21;31(11):2002-2014.e4. doi: 10.1016/j.chembiol.2024.10.006. Epub 2024 Nov 7. PubMed
ATRX silences Cartpt expression in osteoblastic cells during skeletal development. Chen YT, Jiang MM, Leynes C, Adeyeye M, Majano CF, Ibrahim B, Polak U, Hung G, Jin Z, Lanza DG, Liao L, Dawson B, Chen-Evenson Y, Ruiz OE, Gibbons RJ, Heaney JD, Bae Y, Lee B. J Clin Invest. 2025 Jan 2;135(1):e163587. doi: 10.1172/JCI163587. PubMed

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