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Human Lipid Droplet and Metabolism Library
(Pooled Library #191535)

  • Purpose

    Lentiviral CRISPR library targeting 1,196 genes related to lipid droplet biology and cell metabolism. The library contains 13,920 elements (~10 guide RNAs per gene) including 2,000 negative control gRNAs. This library will be useful to researchers in the fields of lipid biology and metabolism.

  • Vector Backbone

    pMCB320 - does not express Cas9


Item Catalog # Description Quantity Price (USD)
Pooled Library 191535 Human Lipid Droplet and Metabolism Library 1 $ 430 Add to Cart
Available to Academic and Nonprofits Only
  • A Cas9 plasmid is NOT included with this item and will have to be ordered separately. Can be used with cells expressing Cas9 or a Cas9-expressing plasmid such as lentiCas9-Blast (Addgene #52962).

Library Details

  • Species
  • Genes targeted
  • gRNAs
  • Controls
    1,000 non-targeting and 1,000 safe guides targeting genomic locations without annotated functions
  • Lentiviral Generation

Library Shipping

This library is delivered as suspended DNA in a microcentrifuge tube on blue ice. The tube's contents will not necessarily be frozen. For best results, minimize freeze-thaws.

  • Volume
    ∼20 µL
  • Concentration
    50 ng/µL
How to cite this pooled library ( Back to top )

These pooled libraries were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Human Lipid Droplet and Metabolism library was a gift from James Olzmann (Addgene #191535)
  • For your References section:

    Parallel CRISPR-Cas9 screens reveal mechanisms of PLIN2 and lipid droplet regulation. Roberts MA, Deol KK, Lange M, Leto DE, Mathiowetz AJ, Stevenson J, Hashemi SH, Morgens DW, Easter E, Heydari K, Nalls MA, Bassik MC, Kampmann M, Kopito RR, Faghri F, Olzmann JA. bioRxiv. 2022.08.27.505556v1. doi: 10.1101/2022.08.27.505556 Article