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Plasmid#160655Purpose"Rescue mfn2 expression by adding the guide-resistant-mutant mfn2 sequence"DepositorInsertmfn2 (MFN2 Human)
UseLentiviralTagsExpressionMutationPromoterCMVAvailable sinceDec. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
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pcDNA3.1-Mfn2
Plasmid#191960PurposeExpression of the mitochondrial GTPase Mfn2.DepositorInsertMfn2
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 Mfn2-HA
Plasmid#139192PurposeExpresses C-terminal HA tagged wt human Mfn2 in mammalian cellsDepositorInsertMFN2 (MFN2 Human)
UseTags3HAExpressionMammalianMutationPromoterCMV promoterAvailable sinceMarch 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pmCherry N1 MFN2
Plasmid#157759PurposeExpression of MFN2:mcherry in cellsDepositorInsertMFN2 (MFN2 Human)
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
Mfn2(K109A)-16xmyc
Plasmid#26051DepositorInsertMfn2 (Mfn2 Mouse)
UseTags16xMycExpressionMammalianMutationK109A GTPase mutantPromoterAvailable sinceAug. 20, 2010AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 Mfn2-HA S27A
Plasmid#139193PurposeExpresses C-terminal HA tagged human Mfn2 S27A in mammalian cellsDepositorInsertMFN2 (MFN2 Human)
UseTags3HAExpressionMammalianMutationSerine 27 to AlaninePromoterCMV promoterAvailable sinceMarch 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 Mfn2-HA S27D
Plasmid#139194PurposeExpresses C-terminal HA tagged human Mfn2 S27D in mammalian cellsDepositorInsertMFN2 (MFN2 Human)
UseTags2HAExpressionMammalianMutationSerine 27 to Aspartic AcidPromoterCMV promoterAvailable sinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMYs-MFN2-IRES-mitoRFP
Plasmid#121997PurposeExpression of MFN2 and mitoRFP in mammalial cellsDepositorInsertMFN2-IRES-mitoRFP (MFN2 Human)
UseRetroviralTagsExpressionMutationsilent Q386Q Q387Q V388V Y389Y C390C E391E E392EPromoterAvailable sinceSept. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1/Hygro-Mfn2-ActA
Plasmid#191981PurposeExpression of Mfn2 tagged with mitochondrion localization signal (ActA)DepositorInsertMfn2-ActA
UseTagsActAExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcB6-Myc-Mfn2 IYFFT
Plasmid#191953PurposeExpression of Mfn2 protein, in which the C-terminus, after the transmembrane domain, is replaced by a stretch of hydrophobic amino acids (IYFFT)DepositorInsertMyc-Mfn2-IYFFT
UseTagsExpressionMammalianMutationC- terminus replaced by the stretch of hydrophobi…PromoterAvailable sinceDec. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pPICZ A-MFN2-Strep (SB253)
Plasmid#227602PurposeInducible expression of Strep-tagged MFN2 in Pichia pastorisDepositorInsertMFN2 (MFN2 Human)
UseTagsTwin-StrepTag, 10xHisExpressionYeastMutationPromoterAOX1Available sinceNov. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
plko-shmfn2-mito-GFP-ER tethering
Plasmid#160509PurposeRescue mfn2 knockdown by tethering mitochondria and ER with GFPDepositorInsertmito-GFP-ER tethering
UseLentiviralTagsExpressionMutationPromoterCMVAvailable sinceNov. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
p3E-U6a-U6c-mfn2 guide
Plasmid#121993PurposeAn entry vector with U6a and U6c promoter driving mfn2 guide RNAs expressionDepositorInsertmfn2 gRNA
UseCRISPRTagsExpressionMutationPromoterAvailable sinceSept. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
p3E-U6a-U6c-mfn2 guide#2
Plasmid#121994PurposeAn entry vector with U6a and U6c promoter driving mfn2 guide RNAs expressionDepositorInsertmfn2 gRNA
UseCRISPRTagsExpressionMutationPromoterAvailable sinceSept. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pPICZ A-SLC[∆2-83]-His+MFN2-Strep (SB259)
Plasmid#227608PurposeInducible coexpression of His-tagged SLC25A46 in its N-terminal region (∆2-83) and Strep-tagged MFN2 in Pichia pastorisDepositorUseTags10xHis and Twin-StrepTagExpressionYeastMutationaa 2-83 deletedPromoterAOX1Available sinceDec. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPICZ A-SLC-His+MFN2[∆TM]-Strep (SB260)
Plasmid#227609PurposeInducible coexpression of His-tagged SLC25A46 and Strep-tagged MFN2 lacking its transmembrane region in Pichia pastorisDepositorUseTags10xHis and Twin-StrepTagExpressionYeastMutationcontains aa 2-544, 651-757 only [TM deleted]PromoterAOX1Available sinceDec. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPICZ A-SLC-His+MFN2-Strep (SB255)
Plasmid#227604PurposeInducible coexpression of His-tagged SLC25A46 and Strep-tagged MFN2 in Pichia pastorisDepositorUseTags10xHis and Twin-StrepTagExpressionYeastMutationPromoterAOX1Available sinceDec. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPICZ A-SLC(2-88)-MBP-His+MFN2-Strep (SB262)
Plasmid#227611PurposeInducible coexpression of His-tagged SLC25A46 truncated to its N-terminal region (2-88) fused to MBP and MFN2-Strep in Pichia pastorisDepositorUseTagsMBP, 10xHis and Twin-StrepTagExpressionYeastMutationcontains aa 2-88 onlyPromoterAOX1Available sinceDec. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPICZ A-SLC-His+MFN2[∆TM,∆HB2]-Strep (SB261)
Plasmid#227610PurposeInducible coexpression of His-tagged SLC25A46 and Strep-tagged MFN2 lacking its transmembrane region and helical bundle 2 (HB2) in Pichia pastorisDepositorUseTags10xHis and Twin-StrepTagExpressionYeastMutationcontains aa 2-400, 706-757 [TM and HB2 deleted]PromoterAOX1Available sinceDec. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
Su9-EGFP
Plasmid#23214DepositorInsertSubunit 9 of the F0-ATPase
UseTagsEGFPExpressionMammalianMutationcontains AA 1-69 of subunit 9PromoterAvailable sinceJan. 28, 2010AvailabilityAcademic Institutions and Nonprofits only -
Cox4-DsRed retrovirus
Plasmid#23215DepositorInsertCox4 (COX4 Budding Yeast)
UseRetroviralTagsDsRedExpressionMutationCox4 mitochondrial targeting sequence fused to Ds…PromoterAvailable sinceJuly 8, 2010AvailabilityAcademic Institutions and Nonprofits only -
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mouse Drp1(K38A)
Plasmid#26049DepositorInsertDrp1 (Dnm1l Mouse)
UseTagsExpressionMammalianMutationK38A GTPase mutantPromoterAvailable sinceSept. 20, 2010AvailabilityAcademic Institutions and Nonprofits only -
Mfn1(K88T)-10xmyc
Plasmid#26050DepositorInsertMfn1 (Mfn1 Mouse)
UseTags10xMycExpressionMammalianMutationK88T GTPase mutantPromoterAvailable sinceAug. 20, 2010AvailabilityAcademic Institutions and Nonprofits only -
pPICZ A-Strep-Opa1 (SB251)
Plasmid#227600PurposeInducible expression of Strep-tagged Opa1 in Pichia pastorisDepositorInsertOPA1 (OPA1 Human)
UseTagsTwin-StrepTagExpressionYeastMutationcontains aa 253-960 onlyPromoterAOX1Available sinceNov. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET-28-Strep-OPA1(MGD) (SB264)
Plasmid#227613PurposeInducible expression of Strep-tagged Opa1(MGD) in E.coliDepositorInsertOPA1 (OPA1 Human)
UseTagsTwin-StrepTagExpressionBacterialMutationcontains aa 253-580 (MGD) and aa 938-960PromoterT7Available sinceDec. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET-28-Strep-OPA1(253-960) (SB263)
Plasmid#227612PurposeInducible expression of Strep-tagged Opa1(253-960) in E.coliDepositorInsertOPA1 (OPA1 Human)
UseTagsTwin-StrepTagExpressionBacterialMutationcontains aa 253-960 onlyPromoterT7Available sinceDec. 6, 2024AvailabilityAcademic Institutions and Nonprofits only