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Showing: 1 - 50 of 247 results

  10. pME-GalT

    Plasmid
    #109558
    Purpose
    Multisite gateway vector for 5' tagging with the N-terminal sequence from zebrafish 1,4 galactosyltransferase 1 (GalT). Marks the Golgi complex.
    Depositor
    Rob Parton
    Article
    Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:
    Insert
    the N-terminal sequence from zebrafish 1,4 galactosyltransferase 1 (GalT)
    Use
    Zebrafish plasmids
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 27, 2020
    Availability
    Academic Institutions and Nonprofits only
    109558-plasmid-map-sequence-id-223872

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  20. pME-GalT

    Plasmid
    #109558
    Purpose
    Multisite gateway vector for 5' tagging with the N-terminal sequence from zebrafish 1,4 galactosyltransferase 1 (GalT). Marks the Golgi complex.
    Depositor
    Rob Parton
    Article
    Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:
    Insert
    the N-terminal sequence from zebrafish 1,4 galactosyltransferase 1 (GalT)
    Use
    Zebrafish plasmids
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 27, 2020
    Availability
    Academic Institutions and Nonprofits only
    109558-plasmid-map-sequence-id-223872

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  23. J-GALTA-3

    Plasmid
    #166523
    Purpose
    scFv of a human scaffold targeting Galactose-1-phosphate uridylyltransferase. Antigen coverage aa 1-379 of 379, full-length
    Depositor
    Susanne Gräslund
    Insert
    single chain-Fv, scFv
    Use
    Recombinant antibody expression
    Tags
    3xFLAG, His6 and OmpA leader sequence
    Expression
    Bacterial
    Mutation
    Promoter
    LacZ
    Available since
    March 31, 2021
    Availability
    Academic Institutions and Nonprofits only
    166523-plasmid-map-sequence-id-329136

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  28. J-GALTA-3

    Plasmid
    #166523
    Purpose
    scFv of a human scaffold targeting Galactose-1-phosphate uridylyltransferase. Antigen coverage aa 1-379 of 379, full-length
    Depositor
    Susanne Gräslund
    Insert
    single chain-Fv, scFv
    Use
    Recombinant antibody expression
    Tags
    3xFLAG, His6 and OmpA leader sequence
    Expression
    Bacterial
    Mutation
    Promoter
    LacZ
    Available since
    March 31, 2021
    Availability
    Academic Institutions and Nonprofits only
    166523-plasmid-map-sequence-id-329136

    Enlarge

  31. piRFP670-N1-GalT

    Plasmid
    #87325
    Purpose
    To express GalT-iRFP670 in mammalian cells, GalT encodes the 1-81 amino acids from β1,4-galactosyltransferase
    Depositor
    Lei Lu
    Article
    Tie et al Mol Biol Cell. 2016 Jan 13. pii: mbc.E15
    Insert
    1-81 amino acids from β1,4-galactosyltransferase (B4GALT1 Human)
    Use
    Tags
    iRFP670
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    May 4, 2017
    Availability
    Academic Institutions and Nonprofits only
    87325-plasmid-map-sequence-id-177296

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  32. Galt1-sCGrx1p-HA

    Plasmid
    #230028
    Purpose
    Expressing sCGrx1p protein fused to HA tag in mammalian cells with localization in the Golgi apparatus.
    Depositor
    Pablo Rivera-Fuentes
    Insert
    Galt1-sCGrx1p-HA (B4GALT1 Synthetic, Human)
    Use
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    230028-plasmid-map-sequence-id-458168

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  33. pmCherry-N1-GalT

    Plasmid
    #87327
    Purpose
    To express GalT-mCherry in mammalian cells, GalT encodes the 1-81 amino acids from β1,4-galactosyltransferase
    Depositor
    Lei Lu
    Article
    Tie et al Mol Biol Cell. 2016 Jan 13. pii: mbc.E15
    Insert
    1-81 amino acids from β1,4-galactosyltransferase (B4GALT1 Human)
    Use
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    May 4, 2017
    Availability
    Academic Institutions and Nonprofits only
    87327-plasmid-map-sequence-id-177633

    Enlarge

  34. lentiMutB3GALT5-blast

    Plasmid
    #208381
    Purpose
    lentiviral vector for expressing human B3GALT5 with C-terminal myc-DDK tag, includes nucleotide change in PAM targeting sequence
    Depositor
    Jennifer Kohler
    Insert
    beta-1,3-galactosyltransferase 5 (B3GALT5 Human)
    Use
    Lentiviral
    Tags
    myc, FLAG
    Expression
    Mammalian
    Mutation
    nucleotide change in PAM targeting sequence nt 51…
    Promoter
    EF-1 alpha core promoter
    Available since
    Sept. 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    208381-plasmid-map-sequence-id-414093

    Enlarge

  35. lentiMutB3GALT5catdead-blast

    Plasmid
    #220869
    Purpose
    lentiviral vector for expressing human B3GALT5 with D156A and D243A inactivating mutations and C-terminal myc-DDK tag, includes nucleotide change in PAM targeting sequence
    Depositor
    Jennifer Kohler
    Insert
    beta-1,3-galactosyltransferase 5 (B3GALT5 Human)
    Use
    Lentiviral
    Tags
    myc, FLAG
    Expression
    Mammalian
    Mutation
    encodes D156A and D243A mutations; also, nucleoti…
    Promoter
    Available since
    Sept. 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    220869-plasmid-map-sequence-id-440865

    Enlarge

  36. GALTA-c001

    Plasmid
    #79645
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    GALT (GALT Human)
    Use
    Tags
    6x His and TEV cleavage site
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    June 16, 2016
    Availability
    Academic Institutions and Nonprofits only
    79645-plasmid-map-sequence-id-150209

    Enlarge

  37. B4GALT1-Dox

    Plasmid
    #124633
    Purpose
    Doxycycline-inducible B4GALT1 expression in mammalian cells
    Depositor
    Ron Weiss
    Article
    Chang et al Nat Chem Biol. 2019 May 20. pii: 10.1038
    Inserts
    Beta-(1,4)-Galactosyltransferase 1
    Tetracycline repressor A3 mutant
    TagFP635
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    TRE-tight and hEF1a
    Available since
    July 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    124633-plasmid-map-sequence-id-248076

    Enlarge

  38. pHA-N1-GalT

    Plasmid
    #87326
    Purpose
    To express GalT-HA in mammalian cells, GalT encodes the 1-81 amino acids from β1,4-galactosyltransferase
    Depositor
    Lei Lu
    Article
    Tie et al Mol Biol Cell. 2016 Jan 13. pii: mbc.E15
    Insert
    1-81 amino acids from β1,4-galactosyltransferase (B4GALT1 Human)
    Use
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Promoter
    CMV-F
    Available since
    May 4, 2017
    Availability
    Academic Institutions and Nonprofits only
    87326-plasmid-map-sequence-id-177295

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  43. pDONR221-B3GALT5

    Plasmid
    #192719
    Purpose
    Gateway entry vector encoding human B3GALT5
    Depositor
    Summer Thyme
    Insert
    B3GALT5 (B3GALT5 Human)
    Use
    Gateway entry vector
    Tags
    Expression
    Mutation
    M85T
    Promoter
    None
    Available since
    Nov. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    192719-plasmid-map-sequence-id-377320

    Enlarge

  44. piRFP670-N1-GalT

    Plasmid
    #87325
    Purpose
    To express GalT-iRFP670 in mammalian cells, GalT encodes the 1-81 amino acids from β1,4-galactosyltransferase
    Depositor
    Lei Lu
    Article
    Tie et al Mol Biol Cell. 2016 Jan 13. pii: mbc.E15
    Insert
    1-81 amino acids from β1,4-galactosyltransferase (B4GALT1 Human)
    Use
    Tags
    iRFP670
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    May 4, 2017
    Availability
    Academic Institutions and Nonprofits only
    87325-plasmid-map-sequence-id-177296

    Enlarge

  45. pmCherry-N1-GalT

    Plasmid
    #87327
    Purpose
    To express GalT-mCherry in mammalian cells, GalT encodes the 1-81 amino acids from β1,4-galactosyltransferase
    Depositor
    Lei Lu
    Article
    Tie et al Mol Biol Cell. 2016 Jan 13. pii: mbc.E15
    Insert
    1-81 amino acids from β1,4-galactosyltransferase (B4GALT1 Human)
    Use
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    May 4, 2017
    Availability
    Academic Institutions and Nonprofits only
    87327-plasmid-map-sequence-id-177633

    Enlarge

  46. lentiMutB3GALT5-blast

    Plasmid
    #208381
    Purpose
    lentiviral vector for expressing human B3GALT5 with C-terminal myc-DDK tag, includes nucleotide change in PAM targeting sequence
    Depositor
    Jennifer Kohler
    Insert
    beta-1,3-galactosyltransferase 5 (B3GALT5 Human)
    Use
    Lentiviral
    Tags
    myc, FLAG
    Expression
    Mammalian
    Mutation
    nucleotide change in PAM targeting sequence nt 51…
    Promoter
    EF-1 alpha core promoter
    Available since
    Sept. 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    208381-plasmid-map-sequence-id-414093

    Enlarge

  47. lentiMutB3GALT5catdead-blast

    Plasmid
    #220869
    Purpose
    lentiviral vector for expressing human B3GALT5 with D156A and D243A inactivating mutations and C-terminal myc-DDK tag, includes nucleotide change in PAM targeting sequence
    Depositor
    Jennifer Kohler
    Insert
    beta-1,3-galactosyltransferase 5 (B3GALT5 Human)
    Use
    Lentiviral
    Tags
    myc, FLAG
    Expression
    Mammalian
    Mutation
    encodes D156A and D243A mutations; also, nucleoti…
    Promoter
    Available since
    Sept. 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    220869-plasmid-map-sequence-id-440865

    Enlarge

  48. GALTA-c001

    Plasmid
    #79645
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    GALT (GALT Human)
    Use
    Tags
    6x His and TEV cleavage site
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    June 16, 2016
    Availability
    Academic Institutions and Nonprofits only
    79645-plasmid-map-sequence-id-150209

    Enlarge

  49. B4GALT1-Dox

    Plasmid
    #124633
    Purpose
    Doxycycline-inducible B4GALT1 expression in mammalian cells
    Depositor
    Ron Weiss
    Article
    Chang et al Nat Chem Biol. 2019 May 20. pii: 10.1038
    Inserts
    Beta-(1,4)-Galactosyltransferase 1
    Tetracycline repressor A3 mutant
    TagFP635
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    TRE-tight and hEF1a
    Available since
    July 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    124633-plasmid-map-sequence-id-248076

    Enlarge

  50. pHA-N1-GalT

    Plasmid
    #87326
    Purpose
    To express GalT-HA in mammalian cells, GalT encodes the 1-81 amino acids from β1,4-galactosyltransferase
    Depositor
    Lei Lu
    Article
    Tie et al Mol Biol Cell. 2016 Jan 13. pii: mbc.E15
    Insert
    1-81 amino acids from β1,4-galactosyltransferase (B4GALT1 Human)
    Use
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Promoter
    CMV-F
    Available since
    May 4, 2017
    Availability
    Academic Institutions and Nonprofits only
    87326-plasmid-map-sequence-id-177295

    Enlarge

Showing: 1 - 50 of 247 results