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zCas9i cloning kit: MoClo-compatible CRISPR/Cas9 cloning kit with intronized Cas9

Plasmid Kit

#1000000171

Purpose

Vectors and DNA modules for assembly of CRISPR/Cas9 constructs for use in plants. The constructs are made with zCas9i, a high efficiency intron-optimized SpCas9-coding gene.

Depositor

Sylvestre Marillonnet, Johannes Stuttmann

Available Since

March 25, 2021

Availability

Academic Institutions and Nonprofits only
Toronto KnockOut Library (TKOv1)

Pooled Library

#1000000069

Purpose

CRISPR gRNA knockout pooled library for targeting the human genome

Depositor

Stephane Angers, Jason Moffat

Available Since

Jan. 28, 2016

Availability

Academic Institutions and Nonprofits only
Toronto KnockOut version 3 library (TKOv3)

Pooled Library

#90294

Purpose

Optimized library offering improved accuracy, efficiency, and scalability for CRISPR screens compared to TKOv1

Depositor

Jason Moffat

Article

Hart et al G3 (Bethesda). 2017 Aug 7;7(8):2719-2727

Available Since

May 5, 2017

Availability

Academic Institutions and Nonprofits only
SARS-CoV-2 RBD Omicron BA.1 SSM Library

Pooled Library

#1000000187

Depositor

Jesse Bloom

Available Since

June 14, 2022

Availability

Academic Institutions and Nonprofits only
SARS-CoV-2 RBD Omicron BA.2 SSM Library

Pooled Library

#1000000188

Depositor

Jesse Bloom

Available Since

June 14, 2022

Availability

Academic Institutions and Nonprofits only
Mouse Toronto KnockOut (mTKO) CRISPR Library - pLCKO2

Pooled Library

#159393

Purpose

Mouse lentiviral genome-wide CRISPR library

Depositor

Jason Moffat

Article

Lawson et al Nature. 2020 Oct;586(7827):120-126. doi:

Expression

Mammalian

Species

Mus musculus

Use

CRISPR and Lentiviral

Available Since

Nov. 9, 2020

Availability

Academic Institutions and Nonprofits only
Mouse Toronto KnockOut (mTKO) CRISPR Library - lentiCRISPRv2

Pooled Library

#159392

Purpose

Mouse lentiviral genome-wide CRISPR library

Depositor

Jason Moffat

Article

Lawson et al Nature. 2020 Oct;586(7827):120-126. doi:

Expression

Mammalian

Species

Mus musculus

Use

CRISPR and Lentiviral

Available Since

Nov. 9, 2020

Availability

Academic Institutions and Nonprofits only
Human Genome-wide Intron Tagging Library, Frame 1

Pooled Library

#216134

Purpose

Library of intron-targeting sgRNAs for pooled tagging of proteins with GFP or other protein tags at their endogenous genomic locus using an intron tagging approach.

Depositor

Stefan Kubicek

Article

Reicher et al Nat Cell Biol. 2024 May;26(5):745-756. d

Expression

Mammalian

Use

CRISPR and Lentiviral

Available Since

April 19, 2024

Availability

Academic Institutions and Nonprofits only
Human Genome-wide Intron Tagging Library, Frame 0

Pooled Library

#216133

Purpose

Library of intron-targeting sgRNAs for pooled tagging of proteins with GFP or other protein tags at their endogenous genomic locus using an intron tagging approach.

Depositor

Stefan Kubicek

Article

Reicher et al Nat Cell Biol. 2024 May;26(5):745-756. d

Expression

Mammalian

Use

CRISPR and Lentiviral

Available Since

April 19, 2024

Availability

Academic Institutions and Nonprofits only
pRL-SV40P

Plasmid

#27163

Purpose

Renilla SV40 promoter (no enhancer) reporter gene for normalization of luciferase experiments

Depositor

Ron Prywes

Article

Chen et al Mol Cell Biol. 1999 Jul . 19(7):4695-702

Insert

SV40 promoter

Use

Luciferase

Tags

Renilla Luciferase

Expression

Mammalian

Available Since

Feb. 25, 2011

Availability

Academic Institutions and Nonprofits only

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pLV_hEF1a_rtTA3

Plasmid

#61472

Purpose

3rd generation lentiviral vector; constitutive expression of rtTA3 (TetON system)

Depositor

Ron Weiss

Article

Busskamp et al Mol Syst Biol. 2014 Nov 17;10(11):760. d

Insert

rtTA3

Use

Lentiviral

Promoter

hEF1a

Available Since

Feb. 2, 2015

Availability

Academic Institutions and Nonprofits only

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GST-PS-Grb2 (XSB363)

Plasmid

#78505

Purpose

Construct for expressing Grb2 in E. Coli

Depositor

Ron Vale

Article

Su et al Science. 2016 Apr 29;352(6285):595-9. do

Insert

Grb2 (GRB2 Human)

Tags

GST followed by a prescission protease claevage s…

Expression

Bacterial

Available Since

June 15, 2016

Availability

Academic Institutions and Nonprofits only

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pTK299

Plasmid

#59568

Purpose

SIN SP6 P1234 (nsP2 P726S) SGP(14) Kozak L7Ae-P2A-EYFP 4xFF4

Depositor

Ron Weiss

Article

Wroblewska et al Nat Biotechnol. 2015 Aug 3. doi: 10.1038

Insert

SGP(14) Kozak L7Ae-P2A-EYFP 4xFF4

Available Since

Aug. 3, 2015

Availability

Academic Institutions and Nonprofits only

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p5xATF6-GL3

Plasmid

#11976

Purpose

Reporter gene with five ATF6 sites that is highly sensitive to ER stress

Depositor

Ron Prywes

Article

Wang et al J Biol Chem. 2000 Sep 1. 275(35):27013-2

Insert

5xATF6 binding site

Tags

Luc and c-fos promoter

Expression

Mammalian

Available Since

Aug. 10, 2007

Availability

Academic Institutions and Nonprofits only

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p3xFLAG-ATF6

Plasmid

#11975

Purpose

For mammalian expression of human ATF6 with a 3x FLAG tag

Depositor

Ron Prywes

Article

Chen et al J Biol Chem. 2002 Apr 12. 277(15):13045-

Insert

ATF6 (ATF6 Human)

Tags

3xFLAG

Expression

Mammalian

Available Since

Aug. 10, 2007

Availability

Academic Institutions and Nonprofits only

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pHRdSV40-dCas9-10xGCN4_v4-P2A-BFP

Plasmid

#60903

Purpose

Expressed a nuclease dead Cas9 tagged with 10 copies of the GCN4 peptide v4 and BFP. This plasmid is part of the SunTag system for gene activation

Depositor

Ron Vale

Article

Tanenbaum et al Cell. 2014 Oct 8. pii: S0092-8674(14)012

Insert

Cas9 dead

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Nov. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pHRdSV40-scFv-GCN4-sfGFP-VP64-GB1-NLS

Plasmid

#60904

Purpose

The plasmid encodes a antibody that binds to the GCN4 peptide from the SunTag system, and is fused to a transcriptional activation domain VP64

Depositor

Ron Vale

Article

Tanenbaum et al Cell. 2014 Oct 8. pii: S0092-8674(14)012

Insert

scFv-GCN4

Use

Lentiviral

Expression

Mammalian

Available Since

Nov. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pLV_TRET_hNgn2_UBC_Puro

Plasmid

#61474

Purpose

3rd generation lentiviral vector; TetON promoter driving human Ngn2; constitutive expression of Puromycin selection marker

Depositor

Ron Weiss

Article

Busskamp et al Mol Syst Biol. 2014 Nov 17;10(11):760. d

Inserts

Ngn2 (NEUROG2 Human)
Puro

Use

Lentiviral

Available Since

Feb. 2, 2015

Availability

Academic Institutions and Nonprofits only

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3XMEF2-luc

Plasmid

#32967

Purpose

Reporter gene for MEF2 activity with 3 MEF2 sites upstream of a c-fos minimal promoter

Depositor

Ron Prywes

Insert

MEF2 binding sites (MEF2A Human)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Promoter

MEF2

Available Since

Feb. 2, 2012

Availability

Academic Institutions and Nonprofits only

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Flag_HsIRE1a_pBabePuro

Plasmid

#54337

Purpose

N-terminally FLAG tagged human IRE1a in retroviral pBabe puro vector

Depositor

David Ron

Article

Volmer et al Proc Natl Acad Sci U S A. 2013 Mar 19;11

Insert

IRE1a (ERN1 Human)

Use

Retroviral

Tags

FLAG and preprotrypsin signal sequence

Expression

Mammalian

Mutation

The endogenous IRE1a signal sequence (aa1-18) was…

Available Since

Sept. 10, 2015

Availability

Academic Institutions and Nonprofits only

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