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zCas9i cloning kit: MoClo-compatible CRISPR/Cas9 cloning kit with intronized Cas9

Plasmid Kit

#1000000171

Purpose

MoClo-compatible vectors for assembly of CRISPR/Cas9 constructs using a high efficiency intron-optimized SpCas9-coding gene in plants.

Depositor

Sylvestre Marillonnet, Johannes Stuttmann

Application

Cloning and Synthetic Biology, Genome Editing

Vector Type

Plant Expression

Editing Type

CRISPR

Cloning Type

Golden Gate (MoClo)

Available Since

March 25, 2021

Availability

Academic Institutions and Nonprofits only
pET17_K560_GFP_His

Plasmid

#15219

Depositor

Ron Vale

Article

Woehlke et al Cell. 1997 Jul 25. 90(2):207-16.

Insert

Human neuronal kinesin heavy chain (KIF5B Human)

Tags

GFP and His

Expression

Bacterial

Available Since

Oct. 12, 2007

Availability

Academic Institutions and Nonprofits only

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pCGN-SRF

Plasmid

#11977

Purpose

For mammalian expression of human SRF with an HA tag

Depositor

Ron Prywes

Article

Johansen et al Mol Cell Biol. 1993 Aug . 13(8):4640-7.

Insert

SRF (SRF Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 10, 2007

Availability

Academic Institutions and Nonprofits only

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p5xATF6-GL3

Plasmid

#11976

Purpose

Reporter gene with five ATF6 sites that is highly sensitive to ER stress

Depositor

Ron Prywes

Article

Wang et al J Biol Chem. 2000 Sep 1. 275(35):27013-2

Insert

5xATF6 binding site

Tags

Luc and c-fos promoter

Expression

Mammalian

Available Since

Aug. 10, 2007

Availability

Academic Institutions and Nonprofits only

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p3xFLAG-ATF6

Plasmid

#11975

Purpose

For mammalian expression of human ATF6 with a 3x FLAG tag

Depositor

Ron Prywes

Article

Chen et al J Biol Chem. 2002 Apr 12. 277(15):13045-

Insert

ATF6 (ATF6 Human)

Tags

3xFLAG

Expression

Mammalian

Available Since

Aug. 10, 2007

Availability

Academic Institutions and Nonprofits only

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pFOS WT-GL3

Plasmid

#11983

Purpose

Reporter gene with mouse c-fos promoter. Induced by serum and many growth factors in quiescent cells.

Depositor

Ron Prywes

Article

Cen et al Mol Cell Biol. 2003 Sep . 23(18):6597-60

Insert

c-fos promoter (Fos Mouse)

Tags

Luc

Expression

Mammalian

Available Since

Aug. 10, 2007

Availability

Academic Institutions and Nonprofits only

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LAMP1-GFP

Plasmid

#16290

Depositor

Ron Vale

Article

Minin et al J Cell Sci. 2006 Feb 15. 119(Pt 4):659-7

Insert

LAMP1/RE72002

Tags

EGFP

Expression

Insect

Promoter

Ac5

Available Since

March 12, 2008

Availability

Academic Institutions and Nonprofits only

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p3xFLAG-MKL1

Plasmid

#11978

Purpose

For mammalian expression of human MKL1 with a 3x FLAG tag

Depositor

Ron Prywes

Article

Cen et al Mol Cell Biol. 2003 Sep . 23(18):6597-60

Insert

MKL1 (MRTFA Human)

Tags

3xFLAG

Expression

Mammalian

Mutation

N579S T716A

Promoter

CMV

Available Since

Aug. 10, 2007

Availability

Academic Institutions and Nonprofits only

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pCGN-ATF6

Plasmid

#11974

Purpose

For mammalian expression of human ATF6 with an HA tag

Depositor

Ron Prywes

Article

Zhu et al Mol Cell Biol. 1997 Sep . 17(9):4957-66.

Insert

ATF6 (ATF6 Human)

Tags

HA

Expression

Mammalian

Available Since

Aug. 10, 2007

Availability

Academic Institutions and Nonprofits only
pJC6-GL3

Plasmid

#11979

Purpose

Reporter gene with mouse c-jun promoter.

Depositor

Ron Prywes

Article

Clarke et al Mol Cell Biol. 1998 Feb . 18(2):1065-73.

Insert

c-jun promoter (Jun Mouse)

Use

Luciferase

Tags

Luc

Expression

Mammalian

Available Since

Aug. 10, 2007

Availability

Academic Institutions and Nonprofits only
pMT-EGFP-Actin5c

Plasmid

#15312

Depositor

Ron Vale

Article

Rogers et al J Cell Biol. 2003 Sep 15. 162(6):1079-88

Insert

Actin5c (Act5C Fly)

Tags

EGFP and His

Expression

Insect

Available Since

Oct. 12, 2007

Availability

Academic Institutions and Nonprofits only
pWBC

Plasmid

#15239

Depositor

Ron Vale

Article

Niclas et al Neuron. 1994 May . 12(5):1059-72.

Insert

Human neuronal kinesin heavy chain (KIF5A Human)

Expression

Bacterial

Available Since

Oct. 12, 2007

Availability

Academic Institutions and Nonprofits only
pXPE

Plasmid

#15284

Depositor

Ron Vale

Article

Navone et al J Cell Biol. 1992 Jun . 117(6):1263-75.

Insert

ubiquitous kinesin heavy chain (KIF5B Human)

Expression

Bacterial

Mutation

The insert is missing the 1st 180 bp of 5' u…

Available Since

Oct. 12, 2007

Availability

Academic Institutions and Nonprofits only
pJTX-GL3

Plasmid

#11981

Purpose

Reporter gene with mouse c-jun promoter and point mutation of AP1 binding site

Depositor

Ron Prywes

Article

Clarke et al Mol Cell Biol. 1998 Feb . 18(2):1065-73.

Insert

c-jun promoter (Jun Mouse)

Use

Luciferase

Tags

Luc

Expression

Mammalian

Available Since

Aug. 10, 2007

Availability

Academic Institutions and Nonprofits only
pJSX-GL3

Plasmid

#11980

Purpose

Reporter gene with mouse c-jun promoter and point mutation of MEF2 binding site

Depositor

Ron Prywes

Article

Clarke et al Mol Cell Biol. 1998 Feb . 18(2):1065-73.

Insert

c-jun promoter (Jun Mouse)

Use

Luciferase

Tags

Luc

Expression

Mammalian

Available Since

Aug. 10, 2007

Availability

Academic Institutions and Nonprofits only
pJSTX-GL3

Plasmid

#11982

Purpose

Reporter gene with mouse c-jun promoter and point mutation of MEF2 and AP1 binding sites

Depositor

Ron Prywes

Article

Clarke et al Mol Cell Biol. 1998 Feb . 18(2):1065-73.

Insert

c-jun promoter (Jun Mouse)

Use

Luciferase

Tags

Luc

Expression

Mammalian

Available Since

Aug. 10, 2007

Availability

Academic Institutions and Nonprofits only
pET_Unc653_GFP_His

Plasmid

#14107

Depositor

Ron Vale

Article

Tomishige et al Science. 2002 Sep 27. 297(5590):2263-7.

Insert

Unc104 (1-653) (unc-104 Nematode)

Tags

GFP and His

Expression

Bacterial

Available Since

Oct. 12, 2007

Availability

Academic Institutions and Nonprofits only
Toronto KnockOut Library (TKOv1)

Pooled Library

#1000000069

Purpose

CRISPR gRNA knockout pooled library for targeting the human genome

Depositor

Stephane Angers, Jason Moffat

Available Since

Jan. 28, 2016

Availability

Academic Institutions and Nonprofits only
Toronto KnockOut version 3 library (TKOv3)

Pooled Library

#90294

Purpose

Optimized library offering improved accuracy, efficiency, and scalability for CRISPR screens compared to TKOv1

Depositor

Jason Moffat

Article

Hart et al G3 (Bethesda). 2017 Aug 7;7(8):2719-2727

Available Since

May 5, 2017

Availability

Academic Institutions and Nonprofits only
SARS-CoV-2 RBD Omicron BA.1 SSM Library

Pooled Library

#1000000187

Depositor

Jesse Bloom

Available Since

June 14, 2022

Availability

Academic Institutions and Nonprofits only