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pSB1A3 EcoRI-RTX with EcoRI Methylase-AmilCP

Plasmid

#85165

Purpose

Provides the coding region to express the EcoRI restriction enzyme. The RTX tag allows the EcoRI enzyme to be isolated via calcium-precipitation.

Depositor

Robin Dowell

Article

Hendrix et al ACS Synth Biol. 2014 Dec 19;3(12):969-71

Insert

EcoRI-RTX and EcoRI Methylase-AmilCP coding sequences

Tags

AmilCP and RTX

Expression

Bacterial

Promoter

J23100 promoter

Available Since

Dec. 20, 2016

Availability

Academic Institutions and Nonprofits only

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pSB1A3 EcoRI-RTX with EcoRI Methylase-AmilCP

Plasmid

#85165

Purpose

Provides the coding region to express the EcoRI restriction enzyme. The RTX tag allows the EcoRI enzyme to be isolated via calcium-precipitation.

Depositor

Robin Dowell

Article

Hendrix et al ACS Synth Biol. 2014 Dec 19;3(12):969-71

Insert

EcoRI-RTX and EcoRI Methylase-AmilCP coding sequences

Tags

AmilCP and RTX

Expression

Bacterial

Promoter

J23100 promoter

Available Since

Dec. 20, 2016

Availability

Academic Institutions and Nonprofits only

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pOpen-EcoRIR

Plasmid

#165504

Purpose

Type II restriction enzyme that recognizes the double-stranded sequence GAATTC and cleaves after G-1.

Depositor

Drew Endy, Jennifer Molloy, FreeGenes Project

Insert

EcoRI

Use

Synthetic Biology

Available Since

July 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pOpen-EcoRIM

Plasmid

#165515

Purpose

Methylase recognizes double-stranded sequence GAATTC, methylates A-3 on both strands, and protects DNA from cleavage by EcoRI.

Depositor

Drew Endy, Jennifer Molloy, FreeGenes Project

Insert

M.EcoRI

Use

Synthetic Biology

Available Since

July 29, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pOpen-EcoRIR

Plasmid

#165504

Purpose

Type II restriction enzyme that recognizes the double-stranded sequence GAATTC and cleaves after G-1.

Depositor

Drew Endy, Jennifer Molloy, FreeGenes Project

Insert

EcoRI

Use

Synthetic Biology

Available Since

July 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pOpen-EcoRIM

Plasmid

#165515

Purpose

Methylase recognizes double-stranded sequence GAATTC, methylates A-3 on both strands, and protects DNA from cleavage by EcoRI.

Depositor

Drew Endy, Jennifer Molloy, FreeGenes Project

Insert

M.EcoRI

Use

Synthetic Biology

Available Since

July 29, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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Prham-M.EcoRI-p15A-aadA

Plasmid

#149341

Purpose

expresses M.EcoRI DNA methyltransferase in E. coli

Depositor

Ichiro Matsumura

Article

Matsumura et al PeerJ. 2020 Sep 11;8:e9841. doi: 10.7717

Insert

M.EcoRI

Expression

Bacterial

Promoter

rhamnose

Available Since

June 19, 2020

Availability

Academic Institutions and Nonprofits only

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pBlue-hFX-HA(NcoI/EcoRI)

Plasmid

#15405

Depositor

Michael Ristow

Insert

human frataxin cDNA (FXN Human)

Tags

AU1 and HA

Expression

Bacterial

Mutation

insert can be released by digestion with REs NcoI…

Available Since

Aug. 17, 2007

Availability

Academic Institutions and Nonprofits only

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pSB1A3 EcoRI Methylase-AmilCP

Plasmid

#85166

Purpose

For methylating EcoRI recognition sites in order to prevent cleavage by the EcoRI restriction enzyme. AmilCP is a blue reporter for Methylase expression.

Depositor

Robin Dowell

Article

Hendrix et al ACS Synth Biol. 2014 Dec 19;3(12):969-71

Insert

EcoRI Methylase-AmilCP

Tags

AmilCP

Expression

Bacterial

Promoter

J23100 promoter

Available Since

Dec. 20, 2016

Availability

Academic Institutions and Nonprofits only
Prham-M.EcoRI-p15A-aadA

Plasmid

#149341

Purpose

expresses M.EcoRI DNA methyltransferase in E. coli

Depositor

Ichiro Matsumura

Article

Matsumura et al PeerJ. 2020 Sep 11;8:e9841. doi: 10.7717

Insert

M.EcoRI

Expression

Bacterial

Promoter

rhamnose

Available Since

June 19, 2020

Availability

Academic Institutions and Nonprofits only

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pBlue-hFX-HA(NcoI/EcoRI)

Plasmid

#15405

Depositor

Michael Ristow

Insert

human frataxin cDNA (FXN Human)

Tags

AU1 and HA

Expression

Bacterial

Mutation

insert can be released by digestion with REs NcoI…

Available Since

Aug. 17, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge
pSB1A3 EcoRI Methylase-AmilCP

Plasmid

#85166

Purpose

For methylating EcoRI recognition sites in order to prevent cleavage by the EcoRI restriction enzyme. AmilCP is a blue reporter for Methylase expression.

Depositor

Robin Dowell

Article

Hendrix et al ACS Synth Biol. 2014 Dec 19;3(12):969-71

Insert

EcoRI Methylase-AmilCP

Tags

AmilCP

Expression

Bacterial

Promoter

J23100 promoter

Available Since

Dec. 20, 2016

Availability

Academic Institutions and Nonprofits only
pAAV-MPRA-MluI-SpeI-EcoRI

Plasmid

#190196

Purpose

Empty vector for inserting MPRA libraries into AAV

Depositor

Hyejung Won

Article

McAfee et al Cell Genom. 2023 Sep 15;3(10):100404. do

Type

Empty backbone

Use

AAV; Massively parallel reporter assay (mpra)

Expression

Mammalian

Available Since

Feb. 7, 2024

Availability

Academic Institutions and Nonprofits only

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pSUPER-retro-puro-shNup35-EcoRI

Plasmid

#87332

Purpose

To express shRNA against human Nup35

Depositor

Lei Lu

Article

Tie et al Biochem Biophys Res Commun. 2016 Sep 30;

Insert

shRNA against human Nup35

Use

RNAi

Expression

Mammalian

Promoter

H1

Available Since

May 1, 2017

Availability

Academic Institutions and Nonprofits only

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pBS-GMR-eya(shRNA)_EcoRImut

Plasmid

#240222

Purpose

pBS-GMR-eya(shRNA) vector with one of two EcoRI sites mutated (T->A)

Depositor

Justin Bosch, Norbert Perrimon

Article

Bosch et al bioRxiv 2025.07.09.659702; doi: https://

Type

Empty backbone

Expression

Insect

Available Since

July 11, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-MPRA-MluI-SpeI-EcoRI

Plasmid

#190196

Purpose

Empty vector for inserting MPRA libraries into AAV

Depositor

Hyejung Won

Article

McAfee et al Cell Genom. 2023 Sep 15;3(10):100404. do

Type

Empty backbone

Use

AAV; Massively parallel reporter assay (mpra)

Expression

Mammalian

Available Since

Feb. 7, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pSUPER-retro-puro-shNup35-EcoRI

Plasmid

#87332

Purpose

To express shRNA against human Nup35

Depositor

Lei Lu

Article

Tie et al Biochem Biophys Res Commun. 2016 Sep 30;

Insert

shRNA against human Nup35

Use

RNAi

Expression

Mammalian

Promoter

H1

Available Since

May 1, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 E2F1 HA(Δ2-126) (HindIII- BamHI- EcoRI)

Plasmid

#70668

Purpose

Human mutant of E2F1 lacking amino acids 2 to 126

Depositor

Maria Alvarado-Kristensson

Article

Hoog et al FASEB J. 2011 Nov;25(11):3815-27. doi: 1

Insert

E2F1 lacking residues 2 to 126 (E2F1 Human)

Tags

HA

Expression

Mammalian

Mutation

lacks amino acids 2-126

Promoter

cmv

Available Since

Nov. 11, 2015

Availability

Academic Institutions and Nonprofits only
pTol1-EcoRI-BmtI-BamHI-cmlc2-nmKate

Plasmid

#238372

Purpose

Empty backbone to generate guideRNA shuttles, that also drives expression of nuclear mKate in cardiomyocytes.

Depositor

Juan Manuel González-Rosa

Article

Keeley et al Cell Rep Methods. 2025 Mar 24;5(3):10100

Insert

nmKate

Promoter

cmlc2 (myl7)

Available Since

May 15, 2025

Availability

Academic Institutions and Nonprofits only

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Prham-M.XbaI-M.EcoRI-M.SalI-p15A-aadA

Plasmid

#170767

Purpose

Methylase expression vector for 5RM assembly: expresses M.XbaI, M.EcoRI and M.SalI DNA methyltransferases in E. coli

Depositor

Ichiro Matsumura

Article

Matsumura et al Biotechniques. 2022 May;72(5):185-193. d

Inserts

M.XbaI
M.EcoRI
M.SalI

Expression

Bacterial

Promoter

rhamnose

Available Since

Feb. 22, 2022

Availability

Academic Institutions and Nonprofits only

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