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Plasmid#23907DepositorInsertSYK (SYK Human)
UseGateway donor vectorTagsExpressionMutationPromoterAvailable sinceJuly 30, 2010AvailabilityAcademic Institutions and Nonprofits only -
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SYKA-c022
Plasmid#175490PurposeProtein expression in bacterial cells. Tandem SH2 domains, M6-N269. Can be biotinylated.DepositorInsertSYK (SYK Human)
UseTagsAviTag and His6-TEVExpressionBacterialMutationPromoterAvailable sinceJan. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
SYKA-c020
Plasmid#175489PurposeProtein expression in bacterial cells. Tandem SH2 domains, M6-N269. Can be used for crystallography.DepositorInsertSYK (SYK Human)
UseTagsHis6-TEVExpressionBacterialMutationPromoterAvailable sinceJan. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
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pMSCV-mCherry-Syk
Plasmid#50045PurposeExpression of mCherry-Syk in mammalian cellsDepositorInsertSpleen tyrosine kinase (Syk) (Syk Mouse)
UseRetroviralTagsmCherryExpressionMammalianMutationPromoterpCMVAvailable sinceJan. 13, 2014AvailabilityAcademic Institutions and Nonprofits only -
pVL1393-MAL-SYK
Plasmid#157990PurposeExpresses SYK in insect cell (sf9)DepositorInsertSpleen tyrosine kinase (SYK Human)
UseTagsMet-Ala-Leu and add Leu,Glu residues +His tagExpressionInsectMutationPromoterAvailable sinceAug. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
Cytosolic Syk Biosensor
Plasmid#125729PurposeFRET biosensor for studying SYK activities in cellsDepositorInsertSYK biosensor (ZAP70 Synthetic)
UseTags6xHistine Tag at the N-terminal.ExpressionMammalianMutationPromoterAvailable sinceNov. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGEX Syk(NC)-SH2
Plasmid#46521DepositorInsertSyk(NC) (SYK Human)
UseTagsGST and PreScissionExpressionBacterialMutationcontains amino acids 7-263PromoterTacAvailable sinceJuly 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGEX Syk(N)-SH2
Plasmid#46520DepositorInsertSyk(N) (SYK Human)
UseTagsGST and PreScissionExpressionBacterialMutationcontains amino acids 7-114PromoterTacAvailable sinceJuly 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGEX Syk(C)-SH2
Plasmid#46519DepositorInsertSyk(C) (SYK Human)
UseTagsGST and PreScissionExpressionBacterialMutationcontains amino acids 149-263PromoterTacAvailable sinceJuly 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
SYK gRNA (BRDN0001146072)
Plasmid#76777Purpose3rd generation lentiviral gRNA plasmid targeting human SYKDepositorInsertSYK (SYK Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
SYK gRNA (BRDN0001148390)
Plasmid#76776Purpose3rd generation lentiviral gRNA plasmid targeting human SYKDepositorInsertSYK (SYK Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
SYK gRNA (BRDN0001146783)
Plasmid#76778Purpose3rd generation lentiviral gRNA plasmid targeting human SYKDepositorInsertSYK (SYK Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGEX6-2 SNAP-Syk tSH2
Plasmid#113028Purposefor purification of Syk tSh2. Purified protein will have a snap tag that can be conjugated to fluorescent dyes.DepositorInsertSNAP-Syk tSH2
UseTagsSNAPExpressionBacterialMutationPromoterAvailable sinceAug. 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET-30a(+)-Syk-tSH2-PM
Plasmid#111271Purposeexpress murine Syk tandem SH2 domains (Ser 8 to Gln 264), with Y130E mutation to mimic phosphorylation, in E.coli strain Rosetta 2 (DE3)DepositorInsertmurine Syk tandem SH2 domains (Ser 8 to Gln 264), with Y130E mutation to mimic phosphorylation (Syk Mouse)
UseTagsExpressionBacterialMutationY130E mutation to mimic phosphorylationPromoterT7 promoterAvailable sinceJuly 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET-30a(+)-Syk-tSH2
Plasmid#111269Purposeexpress murine Syk tandem SH2 domains (Ser 8 to Gln 264), the unphosphorylated form, in E.coli strain Rosetta 2 (DE3)DepositorInsertmurine Syk tandem SH2 domains (Ser 8 to Gln 264), the unphosphorylated form (Syk Mouse)
UseTagsExpressionBacterialMutationPromoterT7Available sinceJuly 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pscALPS-SIV-SYK vpr
Plasmid#115821PurposeEncodes codon optimized 3xFLAG-SIVSYK Vpr (L06042) and puromycin resistance proteinDepositorInsertSIVSYK Vpr (L06042)
UseLentiviralTags3x-FLAGExpressionMutationPromoterSFFVAvailable sinceSept. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET-30a(+)-Syk-tSH2-FX
Plasmid#111272Purposeexpress murine Syk tandem SH2 domains (Ser 8 to Gln 264), with interdomain A (Phe 119 to His 162) substituted by a flexible 20-amino-acid linker (GGS)3GS(GGS)3, in E.coli strain Rosetta 2 (DE3)DepositorInsertmurine Syk tandem SH2 domains (Ser 8 to Gln 264), with interdomain A (Phe 119 to His 162) substituted by a flexible 20aa linker (Syk Mouse)
UseTagsExpressionBacterialMutationinterdomain A (Phe 119 to His 162) substituted by…PromoterT7 promoterAvailable sinceJuly 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pWZL Neo Myr Flag SYK
Plasmid#20646DepositorInsertSYK (SYK Human)
UseRetroviralTagsFlag and MyrExpressionMammalianMutationPromoterAvailable sinceOct. 27, 2009AvailabilityAcademic Institutions and Nonprofits only