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We narrowed to 1,236 results for: poli

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Showing: 361 - 400 of 1236 results

  2. pLV-EF1A-WWE-HA-nLuc-FLAG-T2A-WWE-myc-cLuc-FLAG-IRES-Puro

    Plasmid
    #187611
    Purpose
    Split luciferase construct with nLuc fused to C-terminus of WWE domain, linked by T2A to cLuc fused to C-terminus of WWE domain, linked by IRES to puromycin resistance cassette
    Depositor
    Robert Sobol
    Article
    Koczor et al Cancers (Basel). 2022 Jul 28;14(15). pii
    Inserts
    WWE domain with C-terminal nLuc
    WWE domain with C-terminal cLuc
    Use
    Lentiviral
    Tags
    FLAG, HA, Split luciferase (cLuc), Split lucifera…
    Expression
    Mammalian
    Promoter
    EF1A
    Available Since
    Sept. 15, 2022
    Availability
    Academic Institutions and Nonprofits only
    187611-plasmid-map-sequence-id-372189

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  3. pLV-EF1A-WWE(R163A)-HA-nLuc-FLAG-T2A-WWE-myc-cLuc-FLAG-IRES-Puro

    Plasmid
    #187612
    Purpose
    Split luciferase construct with nLuc fused to C-terminus of WWE domain with Arg163Ala mutation, linked by T2A to cLuc fused to C-terminus of WWE domain, linked by IRES to puromycin resistance cassette
    Depositor
    Robert Sobol
    Article
    Koczor et al Cancers (Basel). 2022 Jul 28;14(15). pii
    Inserts
    WWE domain (R163A) with C-terminal nLuc
    WWE domain with C-terminal cLuc
    Use
    Lentiviral
    Tags
    FLAG, HA, Split luciferase (cLuc), Split lucifera…
    Expression
    Mammalian
    Mutation
    Arg163Ala
    Promoter
    EF1A
    Available Since
    Sept. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    187612-plasmid-map-sequence-id-372035

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  5. PHB1

    Plasmid
    #212153
    Purpose
    For PHB production in E. coli (PT7-pMB1).
    Depositor
    Kang Zhou
    Insert
    poly(3-hydroxyalkanoate) polymerase subunit PhaC, PHA-specific beta-ketothiolase, acetoacetyl-CoA reductase
    Expression
    Bacterial
    Promoter
    PT7
    Available Since
    Jan. 30, 2024
    Availability
    Academic Institutions and Nonprofits only
    212153-plasmid-map-sequence-id-420764

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  6. pcDNA3.1-MS2-HA-HshnRNPE2iso3_Z

    Plasmid
    #148118
    Purpose
    Mammalian Expression of HshnRNPE2iso3
    Depositor
    Elisa Izaurralde
    Insert
    HshnRNPE2iso3 (PCBP2 Human)
    Expression
    Mammalian
    Mutation
    one non silent mutation L239 to S compapred to th…
    Available Since
    March 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    148118-plasmid-map-sequence-id-473831

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  8. pCRII-TOPO CMV-cGFP-SV40 pA + mMALAT1_3'

    Plasmid
    #91803
    Purpose
    Expresses a cGFP mRNA that ends in the MALAT1 triple helix when the upstream SV40 poly(A) signal fails to be used
    Depositor
    Jeremy Wilusz
    Insert
    Coral Green Fluorescent Protein (cGFP)
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    March 31, 2020
    Availability
    Academic Institutions and Nonprofits only
    91803-plasmid-map-sequence-id-180321

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  9. PARP1 FL

    Plasmid
    #169815
    Purpose
    Expresses codon optimised full length His tagged PARP1 in bacterial cells
    Depositor
    David Neuhaus
    Article
    Ogden et al Nucleic Acids Res. 2021 Feb 26;49(4):226
    Insert
    Poly[ADP-ribose] polymerase 1 (PARP1 Synthetic, Codon optimised, Human)
    Tags
    MKHHHHHHMKQ
    Expression
    Bacterial
    Mutation
    Valine 762 mutated to an alanine
    Promoter
    T7 promoter
    Available Since
    Aug. 26, 2021
    Availability
    Academic Institutions and Nonprofits only
    169815-plasmid-map-sequence-id-334563

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  11. pGEX-4T-3-GST-Tev-Af1521(K35E/Y145R)-myc

    Plasmid
    #196241
    Purpose
    N-terminal GST fusion of Af1521(K35E/Y145R) with a TEV protease site located between the GST tag and Af1521 and a myc tag on the C terminus
    Depositor
    Robert Sobol
    Article
    Al-Rahahleh et al DNA Repair (Amst). 2025 Jun;150:103845.
    Insert
    N-terminal GST fusion of Af1521(K35E/Y145R) with a TEV protease site between GST and Af1521 encoding a C-terminal myc tag
    Tags
    GST tag and myc tag
    Expression
    Bacterial
    Mutation
    amino acid 35 lysine is replaced with glutamic ac…
    Available Since
    Aug. 8, 2025
    Availability
    Academic Institutions and Nonprofits only
    196241-plasmid-map-sequence-id-398155

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  13. ATX3 13Q- I77K Q78K W87K

    Plasmid
    #185908
    Purpose
    Expresses ataxin-3 full length with 3 UIMs and 13Q in the Poly-Q track-I77K, Q78K,W87K (N-terminal ) fused with His-Tag and TEV cleavage sequence (C-terminal).
    Depositor
    Sandra Macedo Ribeiro
    Article
    Figueiredo et al Cells. 2022 Jun 19;11(12). pii: cells111
    Insert
    ataxin-3 full length with 3 UIMs and 13Q in the Poly-Q track and point mutations I77K, Q78K and W87K (ATXN3 Synthetic, Human)
    Tags
    6x His-Tag and TEV cleavage site
    Expression
    Bacterial
    Mutation
    Point mutations in Atx3 gene I77K Q78K and W87K
    Available Since
    Aug. 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    185908-plasmid-map-sequence-id-370341

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  15. pT7-V5-SBP-C1-HshnRNPE1

    Plasmid
    #64921
    Purpose
    Expresses V5- and SBP-tagged hnRNPE1 in mammalian cells
    Depositor
    Elisa Izaurralde
    Insert
    hnRNPE1 (PCBP1 Human)
    Tags
    V5 and SBP
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    June 29, 2015
    Availability
    Academic Institutions and Nonprofits only
    64921-plasmid-map-sequence-id-109667

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  16. pT7-V5-SBP-C1-HshnRNPE2iso3_Z

    Plasmid
    #148140
    Purpose
    Mammalian Expression of HshnRNPE2iso3
    Depositor
    Elisa Izaurralde
    Insert
    HshnRNPE2iso3 (PCBP2 Human)
    Expression
    Mammalian
    Mutation
    one non silent mutation L239 to S compapred to th…
    Available Since
    Nov. 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    148140-plasmid-map-sequence-id-385303

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  18. PARP14 (PARP14A-c013)

    Plasmid
    #110254
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    PARP14 (PARP14 Human)
    Tags
    His-TEV
    Expression
    Bacterial
    Promoter
    T7 - lacO
    Available Since
    June 8, 2018
    Availability
    Academic Institutions and Nonprofits only
    110254-plasmid-map-sequence-id-212336

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  20. pAAV-SCP1-dSa VPR mini.-2X snRP-1 BsaI gRNA

    Plasmid
    #99698
    Purpose
    Expresses dSa Cas9 fused to optimized combination of truncated activation domains from SCP1 promoter with dual snRP1 poly adenylation signal, and Sa sgRNA with BsaI cloning sites for programming, vector allows for strong activation of programmed target gene, can be packaged and delivered as AAV
    Depositor
    George Church
    Type
    Empty backbone
    Use
    AAV
    Available Since
    Aug. 31, 2018
    Availability
    Academic Institutions and Nonprofits only
    99698-plasmid-map-sequence-id-194602

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  36. AAA

    Plasmid
    #78487
    Purpose
    2135nt RNA encoded by chimaeric gene cloned in a pSP65A vector that included about 65 dT residues for transcription of a poly(A) tail
    Depositor
    Allan Jacobson
    Article
    Amrani et al Nature. 2008 Jun 26;453(7199):1276-80. d
    Insert
    CAN1-LUC
    Expression
    Bacterial
    Mutation
    CAN1 contains no stop codon
    Available Since
    Aug. 31, 2016
    Availability
    Academic Institutions and Nonprofits only

  38. UAA

    Plasmid
    #78486
    Purpose
    stop codon-containing chimaeric gene cloned in a pSP65A vector that included about 65 dT residues for transcription of a poly(A) tail
    Depositor
    Allan Jacobson
    Article
    Amrani et al Nature. 2008 Jun 26;453(7199):1276-80. d
    Insert
    CAN1-LUC
    Expression
    Bacterial
    Available Since
    Aug. 31, 2016
    Availability
    Academic Institutions and Nonprofits only
Showing: 361 - 400 of 1236 results