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Plasmid#194935PurposeLV construct encoding R103A mutant sNRR-based SynNotch receptorDepositorInsertantiFITC-LaG16-sNRR(R103A)-SynNotch-Gal4-VP64-T2A-BFP
UseLentiviral and Synthetic BiologyTagsExpressionMutationPromoterAvailable sinceMarch 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO_H2B-HaloTag10-T2A-EGFP
Plasmid#175521PurposeNuclear expression of HaloTag10 in mammalian cellsDepositorInsertH2B-HaloTag10
UseTagsExpressionMammalianMutationHaloTag10 = HaloTag7-Q165WPromoterAvailable sinceDec. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pD649-HAsp-JAG1-COMP5AP-AviTag-9xHis
Plasmid#157588PurposeMammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.DepositorInsertJAG1 (JAG1 Human)
UseTagsCOMP5AP-AviTag-9xHisExpressionMammalianMutationPromoterCMV/SP6Available sinceMarch 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
LV anti-FITC-LaG16-Y192A-T2A-BFP
Plasmid#194933PurposeLV construct encoding Y192A mutant sNRR-based SynNotch receptorDepositorInsertantiFITC-LaG16-sNRR(Y192A)-SynNotch-Gal4-VP64-T2A-BFP
UseLentiviral and Synthetic BiologyTagsExpressionMutationPromoterAvailable sinceMarch 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO_H2B-HaloTag11-T2A-EGFP
Plasmid#175522PurposeNuclear expression of HaloTag11 in mammalian cellsDepositorInsertH2B-HaloTag11
UseTagsExpressionMammalianMutationHaloTag11 = HaloTag7-M175WPromoterAvailable sinceDec. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHR-flag-Lag16-fibcon-iCAM1-tether
Plasmid#205213PurposeThis vector encodes of the synCAM tether (fibcon - iCAM1) and GFP nanobody LAG16DepositorInsertsynCAM fibcon - iCAM1 Tether and Lag16 anti-GFP nanobody
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)/BAG1 5′ UTR AUG-nLuc-3xFLAG
Plasmid#127329PurposeExpress 3xFLAG tagged nanoLuciferase (nLuc) from AUG start codon from mRNA with BAG1 5' UTRDepositorInsertnanoLuciferase
UseTags3xFLAGExpressionMammalianMutationPromoterCMVAvailable sinceJune 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)/BAG1 5′ UTR CUG-nLuc-3xFLAG
Plasmid#127328PurposeExpress 3xFLAG tagged nanoLuciferase (nLuc) from CUG start codon from mRNA with BAG1 5' UTRDepositorInsertnanoLuciferase
UseTags3xFLAGExpressionMammalianMutationPromoterCMVAvailable sinceJune 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO_Lifeact-HaloTag11-T2A-Tomm20-HaloTag7
Plasmid#175526PurposeCo-expression of HaloTag11 (actin) and 7 (mitochondrial surface) in mammalian cellsDepositorInsertLifeact-HaloTag11 and Tomm20-HaloTag7
UseTagsExpressionMammalianMutationHaloTag11 = HaloTag7-M175WPromoterAvailable sinceDec. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO_LAMP1-HaloTag11-T2A-Tomn20-HaloTag9
Plasmid#175523PurposeCo-expression of HaloTag11 (lyzosome) and 9 (mitochondrial surface) in mammalian cellsDepositorInsertLAMP1-HaloTag11 and Tomm20-HaloTag9
UseTagsExpressionMammalianMutationHaloTag11 = HaloTag7-M175W and HaloTag9 = HaloTag…PromoterAvailable sinceDec. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO_H2B-HaloTag10-T2A-Tomm20-HaloTag9
Plasmid#175525PurposeCo-expression of HaloTag10 (nuclear) and 9 (mitochondrial surface) in mammalian cellsDepositorInsertH2B-HaloTag10 and Tomm20-HaloTag9
UseTagsExpressionMammalianMutationHaloTag10 = HaloTag7-Q165W and HaloTag9 = HaloTag…PromoterAvailable sinceDec. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPyPGK-Myc-LaG17-SynNotch-tTA-IRES-Ble
Plasmid#183607PurposeMammalian expression vector: mouse Pgk1 promoter driving expression of a SynNotch receptor comprising anti-GFP nanobody (LaG17), mouse Notch1 core, tTA. Confers bleomycin/zeocin resistance.DepositorInsertLaG17 GFP nanobody SynNotch tTA
UseTagsMyc and human CD8a signal sequenceExpressionMammalianMutationPromoterMouse Pgk1Available sinceJune 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
LV anti-FITC-LaG16-Y186F/Y229A-2Xmutant-T2A-BFP
Plasmid#194934PurposeLV construct encoding Y186F/Y229A double mutant sNRR-based SynNotch receptorDepositorInsertantiFITC-LaG16-sNRR(Y186F/Y229A)-SynNotch-Gal4-VP64-T2A-BFP
UseLentiviral and Synthetic BiologyTagsExpressionMutationPromoterAvailable sinceApril 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAS_4xhyb PylT A41AA C55A LaG17-SynNotch 204TAG 442TAA
Plasmid#154779Purposeplasmid with 4xhybrid PylT cassette (mutant A41AA C55A) and LaG17-SynNotch 204TAG 277TAA, for transient transfection or stable, piggybac-mediated, integrationDepositorInsertLaG17-SynNotch
UseTagsLaG17ExpressionMammalianMutation204TAG 442TAA in LaG17-SynNotch, hybrid PylT with…PromoterEF1Available sinceOct. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAS_4xhyb PylT A41AA C55A LaG17-SynNotch 204TAA 442TAG
Plasmid#154778Purposeplasmid with 4xhybrid PylT cassette (mutant A41AA C55A) and LaG17-SynNotch 204TAA 277TAG, for transient transfection or stable, piggybac-mediated, integrationDepositorInsertLaG17-SynNotch
UseTagsLaG17ExpressionMammalianMutation204TAA 442TAG in LaG17-SynNotch, hybrid PylT with…PromoterEF1Available sinceOct. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDSG358
Plasmid#115599Purposesurface-displayed Ag1; medium copyDepositorInsertregulated adhesin construct
UseTagsExpressionBacterialMutationSee depositor comments belowPromoterAvailable sinceOct. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
DMP1-GFPtopaz
Plasmid#114270PurposeGFP labeling of mature osteoblasts and osteocytes.DepositorInsertDmp1 + GFPtopaz (Dmp1 Mouse, Aequorea victoria)
UseTagsExpressionMammalianMutationPromoterDmp1Available sinceMarch 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
coreR1 (384-1008)
Plasmid#115646Purposeexpression of truncated Rag1DepositorInsertRag1 core (384-1008) (Rag1 Mouse)
UseTags8XHis-MBP-PreScissionExpressionMammalianMutationtruncated Rag1 (aa 384-1008)PromoterAvailable sinceSept. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
UR1Ct (265-1040)
Plasmid#115647Purposeexpression of truncated Rag1 including the RING, E3 ubiquitin ligase (265-1008) and C-terminal region (384-1040)DepositorInsertRag1 (265-1040) (Rag1 Mouse)
UseTags8XHis-MBP-PreScissionExpressionMammalianMutationtruncated Rag1 (aa 265-1040)PromoterAvailable sinceOct. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRRL.SIN.EF1A.CD19-FMC63.218.CAR-3G
Plasmid#194458PurposeCAR featuring: GMCSF (sig. peptide); CD8A & CD8A (hinge & TM); CD28, 4-1BB & CD3ζ (signaling domains); anti-CD19 from FMC63 in VL-VH order and 218 linker (scFv). GFP-Zeo for selection and monitoring.DepositorInsertAnti-CD19 CAR
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMarch 20, 2023AvailabilityAcademic Institutions and Nonprofits only