We narrowed to 4,736 results for: donor

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R26-H2B-EGFP HR donor vector

Plasmid

#137925

Purpose

R0SA26 knock-in vector with homologous recombination-based method

Depositor

Hiroshi Kiyonari

Article

Abe et al Cell Rep. 2020 May 19;31(7):107653. doi:

Insert

SA-H2B-EGFP-bpA with homology arms

Use

Mouse Targeting

Available Since

May 19, 2020

Availability

Academic Institutions and Nonprofits only

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AAV_Actb NHEJ donor_U6_sgRNA_EF1a_GFP_polyA

Plasmid

#97310

Purpose

NHEJ donor for fusing a p2A-mCherry reporter to mouse Actb. EGFP driven by EF1a promoter and U6-driven sgRNAs targeting Actb. AAV backbone.

Depositor

Hui Yang

Article

Yao et al Cell Res. 2017 May 19. doi: 10.1038/cr.2

Insert

Actb NHEJ donor

Use

AAV and Mouse Targeting

Expression

Mammalian

Promoter

U6, EF1a

Available Since

Sept. 6, 2017

Availability

Academic Institutions and Nonprofits only

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pJOP-HTT-HR65Q

Plasmid

#92249

Purpose

HTT donor DNA with 65Q for homologous recombination-mediated gene modification

Depositor

Mahmoud Pouladi

Article

Ooi et al Cell Rep. 2019 Feb 26;26(9):2494-2508.e7

Insert

HTT donor DNA (HTT Human)

Use

Precisionx hr targeting vector

Mutation

Barcode of wobble bases to prevent TALEN cleaving…

Available Since

Feb. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pGFPmAID_htz-1

Plasmid

#174214

Purpose

Donor plasmid for tagging htz-1 with GFP::mAID

Depositor

Masato Kanemaki

Article

Negishi et al Genetics. 2021 Dec 2. pii: 6448232. doi:

Insert

GFP::mAID::htz-1 tagging donor

Use

CRISPR

Expression

Worm

Available Since

Dec. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pORANGE GFP-Pclo KI

Plasmid

#139657

Purpose

Endogenous tagging of Piccolo: N-terminal (amino acid position: G2)

Depositor

Harold MacGillavry

Article

Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

Insert

gRNA and GFP donor

Expression

Mammalian

Promoter

U6 and Cbh

Available Since

April 17, 2020

Availability

Academic Institutions and Nonprofits only

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AAV_Dbh HMEJ donor

Plasmid

#97320

Purpose

HMEJ donor for fusing a p2A-mCherry-WPRE reporter to mouse Dbh. AAV backbone.

Depositor

Hui Yang

Article

Yao et al Cell Res. 2017 May 19. doi: 10.1038/cr.2

Insert

Dbh HMEJ donor

Use

AAV and Mouse Targeting

Expression

Mammalian

Available Since

July 13, 2017

Availability

Academic Institutions and Nonprofits only

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pORANGE GFP-Grin1 KI #2

Plasmid

#139658

Purpose

Endogenous tagging of GluN1: N-terminal (amino acid position: D23)

Depositor

Harold MacGillavry

Article

Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

Insert

gRNA and GFP donor

Expression

Mammalian

Promoter

U6 and Cbh

Available Since

April 17, 2020

Availability

Academic Institutions and Nonprofits only

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pJOP-HTT-HR81Q

Plasmid

#92250

Purpose

HTT donor DNA with 81Q for homologous recombination-mediated gene modification

Depositor

Mahmoud Pouladi

Article

Ooi et al Cell Rep. 2019 Feb 26;26(9):2494-2508.e7

Insert

HTT donor DNA (HTT Human)

Use

Precisionx hr targeting vector

Mutation

Barcode of wobble bases to prevent TALEN cleaving…

Available Since

Feb. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pJOP-HTT-HR45Q

Plasmid

#92248

Purpose

HTT donor DNA with 45Q for homologous recombination-mediated gene modification

Depositor

Mahmoud Pouladi

Article

Ooi et al Cell Rep. 2019 Feb 26;26(9):2494-2508.e7

Insert

HTT donor DNA (HTT Human)

Use

Precisionx hr targeting vector

Mutation

Barcode of wobble bases to prevent TALEN cleaving…

Available Since

Feb. 26, 2019

Availability

Academic Institutions and Nonprofits only

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U6_ISCro4_(WT)_DBL_bridge_RNA_polIII_term

Plasmid

#251497

Purpose

Expression of the Donor Binding Loop (DBL) component of the native ISCro4 bridge RNA

Depositor

Martin Jinek

Article

Pelea et al Science. 2026 Feb 5:eadz1884. doi: 10.11

Insert

ISCro4 bridge RNA Donor Binding Loop (DBL)

Expression

Mammalian

Mutation

NA

Available Since

Feb. 5, 2026

Availability

Academic Institutions and Nonprofits only

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pMOD_C2813

Plasmid

#91092

Purpose

Module C, Promoter: PvUbi2, Gene: Csy4 (+ BaeI site for GT donor cloning), Terminator: pea rbcsE9

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

Csy4 (+ BaeI site for GT donor cloning)

Use

CRISPR

Promoter

PvUbi2

Available Since

July 14, 2017

Availability

Academic Institutions and Nonprofits only

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pJOP-HTT-HR30Q

Plasmid

#92247

Purpose

HTT donor DNA with 30Q for homologous recombination-mediated gene modification

Depositor

Mahmoud Pouladi

Article

Ooi et al Cell Rep. 2019 Feb 26;26(9):2494-2508.e7

Insert

HTT donor DNA (HTT Human)

Use

Precisionx hr targeting vector

Mutation

Barcode of wobble bases to prevent TALEN cleaving…

Available Since

Feb. 26, 2019

Availability

Academic Institutions and Nonprofits only

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BFP Homology Donor

Plasmid

#149372

Purpose

Promoter-less BFP cDNA with two homology arms to the lenti-CDDR reporter for HDR detection

Depositor

Tarek Abbas

Article

Eki et al Nucleic Acids Res. 2020 Oct 17. pii: 592

Insert

CDDR BFP Homology Donor

Expression

Mammalian

Available Since

Oct. 7, 2020

Availability

Academic Institutions and Nonprofits only

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Degron-KI donor backbone

Plasmid

#65483

Purpose

Donor backbone vector to clone left and right HR arms in for Degron-KI application

Depositor

Frank Stegmeier

Article

Zhou et al Cancer Res. 2015 Mar 18. pii: canres.293

Type

Empty backbone

Available Since

June 9, 2015

Availability

Academic Institutions and Nonprofits only

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Factor IX donor

Plasmid

#182141

Purpose

Factor IX donor for twinPE and Bxb1 mediated insertion at ALB

Depositor

David Liu

Article

Anzalone et al Nat Biotechnol. 2022 May;40(5):731-740.

Insert

attP-Factor IX intron 1 and exon 2-8 CDS and exon 8 3'UTR

Expression

Mammalian

Available Since

April 21, 2022

Availability

Academic Institutions and Nonprofits only

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pFUGW ORANGE Tubb3-GFP KI _ mCherry-KASH

Plasmid

#131508

Purpose

Endogenous tagging of β3 Tubulin: C-terminal (amino acid position: STOP codon)

Depositor

Harold MacGillavry

Article

Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

Insert

gRNA and GFP donor (Tubb3, Tubb3 Mouse, Rat)

Use

Lentiviral

Expression

Mammalian

Promoter

hUBC

Available Since

Sept. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pSC101-Donor

Plasmid

#140630

Purpose

Kanamycin resistance donor for ShCAST_harboring the temperature sensitive pSC101 origin of replication.

Depositor

Sheng Yang

Article

Zhang et al ACS Synth Biol. 2020 Jun 17. doi: 10.102

Insert

LE, RE, KanR, temperature sensitive pSC101 origin of replication

Use

CRISPR; Transposon

Expression

Bacterial

Available Since

July 17, 2020

Availability

Academic Institutions and Nonprofits only

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pMOD_C2806

Plasmid

#91091

Purpose

Module C, Promoter: FMV 34S, Gene: Csy4 (+ BaeI site for GT donor cloning), Terminator: pea rbcsE9

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

Csy4 (+ BaeI site for GT donor cloning)

Use

CRISPR

Promoter

FMV 34S

Available Since

July 14, 2017

Availability

Academic Institutions and Nonprofits only

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pDonor14-N7HELIX-KanR

Plasmid

#190663

Purpose

Kanamycin resistance donor for N7HELIX containing 14bp of spacing between the I-AniI site and LE/RE using ShCAST flanking sequence

Depositor

Benjamin Kleinstiver

Article

Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

Insert

KanR, R6K origin of replication

Expression

Bacterial

Promoter

NA

Available Since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pAGD289

Plasmid

#228113

Purpose

pET-21(+) deriviative with retron Kva1 containing a donor to edit E. coli rpoB gene cloned downstream of a T7 promoter

Depositor

Seth Shipman

Article

Khan et al Nat Biotechnol. 2024 Sep 17. doi: 10.103

Insert

Kva1 retron, ncRNA with 90 bp rpoB donor

Expression

Bacterial

Promoter

T7

Available Since

Dec. 5, 2024

Availability

Academic Institutions and Nonprofits only

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