Addgene

We narrowed to 6,011 results for: gca

Showing: 181 - 200 of 6011 results

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pCS2-F2-GCA

Plasmid

#30720

Depositor

Nathan Lawson, Scot Wolfe

Article

Zhu et al Development. 2011 Oct;138(20):4555-64.

Insert

F2 GCA

Use

Mrna production

Tags

HA

Expression

Mutation

Promoter

Available since

Nov. 9, 2011

Availability

Academic Institutions and Nonprofits only
pCS2-F3-GCA

Plasmid

#30754

Depositor

Nathan Lawson, Scot Wolfe

Article

Zhu et al Development. 2011 Oct;138(20):4555-64.

Insert

F3 GCA

Use

Mrna production

Tags

FLAG

Expression

Mutation

Promoter

Available since

Nov. 9, 2011

Availability

Academic Institutions and Nonprofits only
pCS2-F1-GCA

Plasmid

#30697

Depositor

Nathan Lawson, Scot Wolfe

Article

Zhu et al Development. 2011 Oct;138(20):4555-64.

Insert

F1 GCA

Use

Mrna production

Tags

HA tag

Expression

Mutation

Promoter

Available since

Nov. 9, 2011

Availability

Academic Institutions and Nonprofits only
ER-GCaMP6-150

Plasmid

#86918

Purpose

Fluorescent reporter for ER calcium signaling

Depositor

Timothy Ryan

Article

de Juan-Sanz et al Neuron. 2017 Jan 30. pii: S0896-6273(17)

Insert

ER-GCaMP6-150

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

K78H, T302L, R303P, A317E, D324G, D360G, D380Y, T…

Promoter

CAMKII

Available since

March 15, 2017

Availability

Academic Institutions and Nonprofits only

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ER-GCaMP6-210

Plasmid

#86919

Purpose

Fluorescent reporter for ER calcium signaling

Depositor

Timothy Ryan

Article

de Juan-Sanz et al Neuron. 2017 Jan 30. pii: S0896-6273(17)

Insert

ER-GCaMP6-210

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

K78H, T302L, R303P, A317E, D324G, D360G, D380Y, T…

Promoter

CAMKII

Available since

March 15, 2017

Availability

Academic Institutions and Nonprofits only

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pGCaMP6m-N3-TPC2

Plasmid

#80147

Purpose

GCaMP6 is a genetically encoded fluorescent Ca2+ sensor . TPC2-GCaMP6 allows the visualization of Ca2+ release from intracellular Ca2+ stores in real time

Depositor

Santiago Di Pietro

Article

Ambrosio et al Mol Biol Cell. 2015 Sep 15;26(18):3263-7

Insert

TPCN2 (TPCN2 Human)

Use

Tags

GCaMP6m

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 18, 2016

Availability

Academic Institutions and Nonprofits only

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pAAVS1-PC-GCaMP6f

Plasmid

#73503

Purpose

GCaMP6f knock in construct into the AAVS1 locus

Depositor

Bruce Conklin

Article

Mandegar et al Cell Stem Cell. 2016 Apr 7;18(4):541-53.

Insert

GCaMP6f

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Dec. 7, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3-ER-GCaMP3

Plasmid

#64854

Purpose

Endoplasmic reticulum (ER)-targeted version of the Ca2+ indicator GCaMP3; targeted to cytosolic face of ER membrane.

Depositor

Jin Zhang

Article

Mehta et al eLife. 2014 Jul 23;3:e03765. doi: 10.755

Insert

CYP450-GCaMP3

Use

Tags

N-terminal 27 amino acids from CYP450

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 8, 2015

Availability

Academic Institutions and Nonprofits only

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Orai1-GCaMP6f

Plasmid

#73564

Purpose

Green fluorescent reporter of Orai1-associated calcium influx

Depositor

Michael Cahalan

Article

Dynes et al Proc Natl Acad Sci U S A. 2016 Jan 12;11

Insert

Orai1-GCaMP6f (ORAI1 Human, Synthetic)

Use

Tags

GCaMP6f

Expression

Mammalian

Mutation

Promoter

CMV Immediate Early

Available since

March 11, 2016

Availability

Academic Institutions and Nonprofits only

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pCMV-GCaMP5G

Plasmid

#31788

Purpose

a single-wavelength GCaMP3-based calcium indicator with improved response. Please also see the GCaMP6s/m/f indicators.

Depositor

Douglas Kim, Loren Looger, GENIE Project

Article

Akerboom et al J Neurosci. 2012 Oct 3;32(40):13819-40.

Insert

GCaMP5G

Use

Tags

6xHis, T7 epitope, and Xpress tag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 22, 2011

Availability

Academic Institutions and Nonprofits only

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pRSET-GCaMP6f

Plasmid

#67564

Purpose

bacterial expression of a fluorescent calcium reporter

Depositor

Ehud Isacoff

Article

Berlin et al Nat Methods. 2015 Jul 13. doi: 10.1038/n

Insert

GCaMP6f

Use

Tags

HIS-tag

Expression

Bacterial

Mutation

Promoter

Available since

July 29, 2015

Availability

Academic Institutions and Nonprofits only
MAP2-GCaMP3

Plasmid

#43917

Depositor

John Gearhart

Article

Addis et al PLoS One. 2011;6(12):e28719. doi: 10.137

Insert

GCaMP3

Use

Lentiviral

Tags

6xHis, T7 epitope, and Xpress tag

Expression

Mammalian

Mutation

E247V in GCaMP3

Promoter

Human MAP2 promoter

Available since

May 10, 2013

Availability

Academic Institutions and Nonprofits only

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nGCaMP2-NpuDnaEn

Plasmid

#34850

Depositor

Kevin Truong

Article

Wong et al Cell Calcium. 2011 Nov 29.

Insert

nGCaMP2-NpuDnaEn

Use

Tags

Expression

Bacterial and Mammalian

Mutation

Promoter

CMV, T7 lac

Available since

March 27, 2012

Availability

Academic Institutions and Nonprofits only
NpuDnaEc-cGCaMP2

Plasmid

#34851

Depositor

Kevin Truong

Article

Wong et al Cell Calcium. 2011 Nov 29.

Insert

NpuDnaEc-cGCaMP2

Use

Tags

Expression

Bacterial and Mammalian

Mutation

Promoter

CMV, T7 lac

Available since

June 21, 2012

Availability

Academic Institutions and Nonprofits only
pCMV-GCaMP3bright

Plasmid

#74423

Purpose

Express fluorescent calcium indicator GCaMP3bright in mammalian cells

Depositor

Katalin Torok

Article

Helassa et al Sci Rep. 2015 Nov 3;5:15978. doi: 10.103

Insert

GCaMP3bright

Use

Tags

Expression

Mammalian

Mutation

D392A

Promoter

CMV

Available since

May 12, 2016

Availability

Academic Institutions and Nonprofits only

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pCMV-GCaMP3fast

Plasmid

#74422

Purpose

Express fluorescent calcium indicator GCaMP3fast in mammalian cells

Depositor

Katalin Torok

Article

Helassa et al Sci Rep. 2015 Nov 3;5:15978. doi: 10.103

Insert

GCaMP3fast

Use

Tags

Expression

Mammalian

Mutation

W4Y D356A

Promoter

CMV

Available since

May 12, 2016

Availability

Academic Institutions and Nonprofits only

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5HT6-GCaMP5G

Plasmid

#48340

Purpose

Fluorescent reporter for Ca2+ inside primary cilia

Depositor

Takanari Inoue

Article

Su et al Nat Methods. 2013 Sep 22. doi: 10.1038/n

Insert

GCaMP5G

Use

Tags

5HT6

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 26, 2013

Availability

Academic Institutions and Nonprofits only
CMV::ratSyGCaMP2

Plasmid

#26124

Depositor

Leon Lagnado

Article

Dreosti et al Nat Methods. 2009 Dec . 6(12):883-9.

Insert

Synaptophysin GCaMP2 (Syp Rat)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 11, 2010

Availability

Academic Institutions and Nonprofits only

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pCMV-GCaMP6fu

Plasmid

#86235

Purpose

Express fluorescent calcium indicator GCaMP6fu in mammalian cells

Depositor

Katalin Torok

Article

Helassa et al Sci Rep. 2016 Dec 6;6:38276. doi: 10.103

Insert

GCaMP6fu

Use

Tags

Expression

Mammalian

Mutation

W43Y D395A

Promoter

CMV

Available since

Jan. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pActP-GCaMP6m

Plasmid

#64714

Purpose

visualizing calcium in cultured Drosophila S2 cells

Depositor

Liqun Luo

Article

Gao et al Nat Neurosci. 2015 May 11. doi: 10.1038/

Insert

GCaMP6m

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

May 22, 2015

Availability

Academic Institutions and Nonprofits only

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