We narrowed to 3,216 results for: pam

1. p11-3’_8xN_PAM-site2 (RTW555)

   Pooled Library

   #160133

   Purpose

   Plasmid library harboring a randomized 8nt PAM 3’ of target site 2. Can be used to profile CRISPR-Cas PAM requirements of enzymes that recognize 3’ PAMs (e.g. Cas9)

   Depositor

   Benjamin Kleinstiver, Keith Joung

   Article

   Walton et al Nat Protoc. 2021 Feb 5. pii: 10.1038/s41

   Species

   Synthetic

   Use

   CRISPR

   Available since

   Feb. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

2. pAM-CAGGS-FLEX-H2B-3XHA-P2A-N2c(G)

   Plasmid

   #73477

   Purpose

   Encodes the G protein to enable transsynaptic RAV infection

   Depositor

   Thomas Jessell

   Article

   Reardon et al Neuron. 2016 Jan 19. pii: S0896-6273(16)

   Insert

   H2B-3XHA-P2A-N2c(G)
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAM-CAGGS-FLEX-H2B-GFP-P2A-N2c(G)

   Plasmid

   #73475

   Purpose

   Encodes the G protein to enable transsynaptic RAV infection

   Depositor

   Thomas Jessell

   Article

   Reardon et al Neuron. 2016 Jan 19. pii: S0896-6273(16)

   Insert

   H2B-GFP-P2A-N2c(G)
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 21, 2016

   Availability

   Academic Institutions and Nonprofits only

4. pCIneo-PAM-sig-His6-gamma-PALcc-deltaGlyc

   Plasmid

   #25458

   Depositor

   Betty Eipper

   Article

   Chufan et al Structure. 2009 Jul 15. 17(7):965-73.

   Insert

   PAM (Pam Rat)
   

   Use

   Tags

   His6 and PAM signal

   Expression

   Mammalian

   Mutation

   Contains aa 498-820 (PALcc= PAL catalytic core) …

   Promoter

   Available since

   July 13, 2010

   Availability

   Academic Institutions and Nonprofits only

5. pAM5406

   Plasmid

   #132661

   Purpose

   RSF1010-based broad host-range plasmid with additional RK2 bom site and pUC origin

   Depositor

   Susan Golden

   Article

   Bishe et al iScience. 2019 Oct 25;20:216-228. doi: 1

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAM5404

   Plasmid

   #132660

   Purpose

   RSF1010-based broad host-range plasmid with additional RK2 bom site

   Depositor

   Susan Golden

   Article

   Bishe et al iScience. 2019 Oct 25;20:216-228. doi: 1

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pAM5409

   Plasmid

   #132662

   Purpose

   RSF1010-based broad host-range plasmid with RK2 bom site expressing YFP and Sp/Sm resistance

   Depositor

   Susan Golden

   Article

   Bishe et al iScience. 2019 Oct 25;20:216-228. doi: 1

   Inserts

   aadA
   YFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PaadA and PconII

   Available since

   Nov. 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAM5505

   Plasmid

   #132664

   Purpose

   pRL623-based helper plasmid for conjugations, with RSF1010 mobA gene

   Depositor

   Susan Golden

   Article

   Bishe et al iScience. 2019 Oct 25;20:216-228. doi: 1

   Insert

   MobA
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   please see depositor comments below

   Promoter

   p1,p3

   Available since

   May 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pJethlpAmScarletCm

   Plasmid

   #206818

   Purpose

   Template for hlpA-mScarlet-I amplification associated with chloramphenicol resistance gene

   Depositor

   Virginie Libante

   Article

   Mignolet et al bioRxiv 2025.05.10.653270

   Insert

   hlpA
   

   Use

   Synthetic Biology

   Tags

   mScarlet

   Expression

   Mutation

   Promoter

   Available since

   May 12, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pJethlpAmNeonGreenCm

    Plasmid

    #206814

    Purpose

    Template for hlpA-mneongreen amplification associated with chloramphenicol resistance gene

    Depositor

    Virginie Libante

    Article

    Mignolet et al bioRxiv 2025.05.10.653270

    Insert

    hlpA
    

    Use

    Synthetic Biology

    Tags

    mNeonGreen

    Expression

    Mutation

    3insG

    Promoter

    Available since

    May 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pJethlpAmNeonGreenKm

    Plasmid

    #206816

    Purpose

    Template for hlpA-mneongreen amplification associated with kanamycin resistance gene

    Depositor

    Virginie Libante

    Article

    Mignolet et al bioRxiv 2025.05.10.653270

    Insert

    hlpA
    

    Use

    Synthetic Biology

    Tags

    mNeonGreen

    Expression

    Mutation

    Promoter

    Available since

    May 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pAM5087

    Plasmid

    #85123

    Purpose

    Ptrc-YFP subcloned from pAM5080 into NSII-Km vector.

    Depositor

    Susan Golden

    Article

    Cohen et al Curr Biol. 2014 Aug 18;24(16):1836-44. d

    Insert

    YFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. M3-PAmcherry1

    Plasmid

    #114189

    Purpose

    studying clustering of M3 receptors by superresolution microscopy (PALM)

    Depositor

    Aleksandra Radenovic

    Insert

    Muscarin acetylcholine receptor 3 (CHRM3 Human)
    

    Use

    Tags

    3xHA and PAmCherry

    Expression

    Mammalian

    Mutation

    Promoter

    SV40

    Available since

    Oct. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAM1

    Plasmid

    #51401

    Purpose

    Expresses truncated version of NLL-MetRS from endogenous MetG promoter

    Depositor

    David Tirrell

    Article

    Mahdavi et al Proc Natl Acad Sci U S A. 2014 Jan 7;111

    Insert

    MetRS (metG E coli)
    

    Use

    Tags

    Expression

    Mutation

    L13N, Y260L, H301L; Please note that a truncated …

    Promoter

    MetG

    Available since

    March 13, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAM2991

    Plasmid

    #40248

    Depositor

    Susan Golden

    Article

    Ivleva et al EMBO J. 2005 Mar 23;24(6):1202-10. Epub

    Insert

    One Step Cloneing Vector for Overexpression
    

    Use

    Cyanobacteria cloning vector

    Tags

    Expression

    Mutation

    Promoter

    n/a

    Available since

    March 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. pPAmCherry3-C1

    Plasmid

    #31933

    Depositor

    Vladislav Verkhusha

    Article

    Subach et al Nat Methods. 2009 Feb;6(2):153-9. Epub 2

    Insert

    PAmCherry3
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    M18L/A58T/K69N/L84F/M137I/E146D/S148L/I165L/Q167A…

    Promoter

    CMV

    Available since

    Sept. 12, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pPAmCherry2-C1

    Plasmid

    #31932

    Depositor

    Vladislav Verkhusha

    Article

    Subach et al Nat Methods. 2009 Feb;6(2):153-9. Epub 2

    Insert

    PAmCherry2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    M18L/K69N/L84F/A147T/S148L/I165L/Q167A/L169T/A179…

    Promoter

    CMV

    Available since

    Sept. 12, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. PAM1

    Plasmid

    #198740

    Purpose

    pTFIID-GFP, Geneticin resistance

    Depositor

    Chantal Abergel

    Article

    Philippe et al Nat Protoc. 2023 Nov 14. doi: 10.1038/s4

    Insert

    GFP
    

    Use

    Acanthamoeba expression

    Tags

    Expression

    Mutation

    Promoter

    pTFIID

    Available since

    July 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAM5411

    Plasmid

    #132663

    Purpose

    RSF1010-based broad host-range plasmid with RK2 bom site and pUC origin expressing YFP and Sp/Sm resistance

    Depositor

    Susan Golden

    Article

    Bishe et al iScience. 2019 Oct 25;20:216-228. doi: 1

    Inserts

    aadA
    YFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PaadA and PconII

    Available since

    Nov. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pPAmCherry1-N1

    Plasmid

    #31928

    Depositor

    Vladislav Verkhusha

    Article

    Subach et al Nat Methods. 2009 Feb;6(2):153-9. Epub 2

    Insert

    PAmCherry1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    E26V/A58T/K69N/L84F/N99K/S148L/I165V/Q167P/L169V/…

    Promoter

    cmv

    Available since

    Sept. 12, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge