We narrowed to 28,832 results for: NTS

1. pMDC32B-B/c

   Plasmid

   #227963

   Purpose

   Expression plasmid with a ccdB cassette flanked with two inverted BsaI restriction sites. Allows the high throughput cloning of cloning inserts flanked with compatible 4-nt overhangs.

   Depositor

   Alberto Carbonell

   Article

   Cisneros et al Nucleic Acids Res. 2025 Feb 27;53(5):gka

   Insert

   B/c
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   35S

   Available since

   April 22, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pMDC32B-B/c

   Plasmid

   #227963

   Purpose

   Expression plasmid with a ccdB cassette flanked with two inverted BsaI restriction sites. Allows the high throughput cloning of cloning inserts flanked with compatible 4-nt overhangs.

   Depositor

   Alberto Carbonell

   Article

   Cisneros et al Nucleic Acids Res. 2025 Feb 27;53(5):gka

   Insert

   B/c
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   35S

   Available since

   April 22, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCRISPEY-Z3-Nat-ADE2

   Plasmid

   #232106

   Purpose

   Expresses estradiol-inducible CRISPR guide RNA and repair template for creating a frameshift mutation in ADE2 gene of yeast.

   Depositor

   Jeffrey Lewis

   Article

   Stuecker et al G3 (Bethesda). 2025 Aug 4:jkaf175. doi:

   Insert

   ADE2 gRNA and repair template
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Repair template introduces C at nt 466 of ADE2 to…

   Promoter

   Z3

   Available since

   Feb. 21, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCRISPEY-Z3-Nat-ADE2

   Plasmid

   #232106

   Purpose

   Expresses estradiol-inducible CRISPR guide RNA and repair template for creating a frameshift mutation in ADE2 gene of yeast.

   Depositor

   Jeffrey Lewis

   Article

   Stuecker et al G3 (Bethesda). 2025 Aug 4:jkaf175. doi:

   Insert

   ADE2 gRNA and repair template
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Repair template introduces C at nt 466 of ADE2 to…

   Promoter

   Z3

   Available since

   Feb. 21, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCRISPEY-GAL-Hyg-ADE2

   Plasmid

   #232104

   Purpose

   Expresses galactose-inducible CRISPR guide RNA and repair template for creating a frameshift mutation in ADE2 gene of yeast.

   Depositor

   Jeffrey Lewis

   Article

   Stuecker et al G3 (Bethesda). 2025 Aug 4:jkaf175. doi:

   Insert

   ADE2 gRNA and repair template
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Repair template introduces C at nt 466 of ADE2 to…

   Promoter

   GAL7

   Available since

   Feb. 21, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCRISPEY-GAL-Hyg-ADE2

   Plasmid

   #232104

   Purpose

   Expresses galactose-inducible CRISPR guide RNA and repair template for creating a frameshift mutation in ADE2 gene of yeast.

   Depositor

   Jeffrey Lewis

   Article

   Stuecker et al G3 (Bethesda). 2025 Aug 4:jkaf175. doi:

   Insert

   ADE2 gRNA and repair template
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Repair template introduces C at nt 466 of ADE2 to…

   Promoter

   GAL7

   Available since

   Feb. 21, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCRISPEY-Z3-Hyg-ADE2

   Plasmid

   #232107

   Purpose

   Expresses estradiol-inducible CRISPR guide RNA and repair template for creating a frameshift mutation in ADE2 gene of yeast.

   Depositor

   Jeffrey Lewis

   Article

   Stuecker et al G3 (Bethesda). 2025 Aug 4:jkaf175. doi:

   Insert

   ADE2 gRNA and repair template
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Repair template introduces C at nt 466 of ADE2 to…

   Promoter

   Z3

   Available since

   Feb. 21, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCRISPEY-Z3-Hyg-ADE2

   Plasmid

   #232107

   Purpose

   Expresses estradiol-inducible CRISPR guide RNA and repair template for creating a frameshift mutation in ADE2 gene of yeast.

   Depositor

   Jeffrey Lewis

   Article

   Stuecker et al G3 (Bethesda). 2025 Aug 4:jkaf175. doi:

   Insert

   ADE2 gRNA and repair template
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Repair template introduces C at nt 466 of ADE2 to…

   Promoter

   Z3

   Available since

   Feb. 21, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. TL1 alphaDdCBE A1 (DddA6-C) - TL1 alphaL_DdCBE 2 (left, DddA11-C)

   Plasmid

   #221994

   Purpose

   Expresses mitochondrial cytosine base editor arm in mammalian cells

   Depositor

   Stephen Ekker

   Article

   Castillo et al Hum Gene Ther. 2024 Sep 24. doi: 10.1089

   Insert

   COX8A MTS-3xFLAG-TALE NT-alphaN-TL1 TALE A1 repeats-TALE CTD-DddA6/11 1397C-UGI-ATP5B 3'UTR
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   TALE: Q231R, W232G, S233A | DddA: T1413I

   Promoter

   CMV

   Available since

   Sept. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. TL1 alphaDdCBE A1 (DddA6-C) - TL1 alphaL_DdCBE 2 (left, DddA11-C)

    Plasmid

    #221994

    Purpose

    Expresses mitochondrial cytosine base editor arm in mammalian cells

    Depositor

    Stephen Ekker

    Article

    Castillo et al Hum Gene Ther. 2024 Sep 24. doi: 10.1089

    Insert

    COX8A MTS-3xFLAG-TALE NT-alphaN-TL1 TALE A1 repeats-TALE CTD-DddA6/11 1397C-UGI-ATP5B 3'UTR
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    TALE: Q231R, W232G, S233A | DddA: T1413I

    Promoter

    CMV

    Available since

    Sept. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pGEM-601[insert(+1) TA-rich]

    Plasmid

    #221196

    Purpose

    601 nucleosome positioning sequence with an additional base pair inserted 22 nt from the dyad on the TA-rich side of the 601

    Depositor

    Gregory Bowman

    Article

    Nodelman et al Nat Struct Mol Biol. 2022 Feb;29(2):121-

    Insert

    601[insert(+1)TA-rich]
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    601 has an additional mutation added 22 bp from d…

    Promoter

    Available since

    Aug. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pGEM-601[insert(+1) TA-rich]

    Plasmid

    #221196

    Purpose

    601 nucleosome positioning sequence with an additional base pair inserted 22 nt from the dyad on the TA-rich side of the 601

    Depositor

    Gregory Bowman

    Article

    Nodelman et al Nat Struct Mol Biol. 2022 Feb;29(2):121-

    Insert

    601[insert(+1)TA-rich]
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    601 has an additional mutation added 22 bp from d…

    Promoter

    Available since

    Aug. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pUPD2 linker-RDF (GB2892)

    Plasmid

    #160604

    Purpose

    RDF sequence for C-terminal fusion with PhiC31 recombinase, together with a 18-nt linker sequence

    Depositor

    Diego Orzaez

    Article

    Bernabe-Orts et al Nucleic Acids Res. 2020 Apr 6;48(6):3379

    Insert

    RDF
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Available since

    Jan. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pUPD2 linker-RDF (GB2892)

    Plasmid

    #160604

    Purpose

    RDF sequence for C-terminal fusion with PhiC31 recombinase, together with a 18-nt linker sequence

    Depositor

    Diego Orzaez

    Article

    Bernabe-Orts et al Nucleic Acids Res. 2020 Apr 6;48(6):3379

    Insert

    RDF
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Available since

    Jan. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pC0.178

    Plasmid

    #119645

    Purpose

    Level 0 Part. Insertion site

    Depositor

    Alistair McCormick

    Article

    Vasudevan et al Plant Physiol. 2019 May;180(1):39-55. do

    Insert

    7942 NS3 Down Flank
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    4 nucleotide insertion between nt 65 and 66 in NS…

    Promoter

    Available since

    Oct. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pC0.178

    Plasmid

    #119645

    Purpose

    Level 0 Part. Insertion site

    Depositor

    Alistair McCormick

    Article

    Vasudevan et al Plant Physiol. 2019 May;180(1):39-55. do

    Insert

    7942 NS3 Down Flank
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    4 nucleotide insertion between nt 65 and 66 in NS…

    Promoter

    Available since

    Oct. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pC0.165

    Plasmid

    #119634

    Purpose

    Level 0 Part. Insertion site

    Depositor

    Alistair McCormick

    Article

    Vasudevan et al Plant Physiol. 2019 May;180(1):39-55. do

    Insert

    6803 NS1 Up Flank
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    1 nucleotide insertion between nt 21 and 22 in NS…

    Promoter

    Available since

    Oct. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pC0.165

    Plasmid

    #119634

    Purpose

    Level 0 Part. Insertion site

    Depositor

    Alistair McCormick

    Article

    Vasudevan et al Plant Physiol. 2019 May;180(1):39-55. do

    Insert

    6803 NS1 Up Flank
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    1 nucleotide insertion between nt 21 and 22 in NS…

    Promoter

    Available since

    Oct. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pC0.175

    Plasmid

    #119643

    Purpose

    Level 0 Part. Insertion site

    Depositor

    Alistair McCormick

    Article

    Vasudevan et al Plant Physiol. 2019 May;180(1):39-55. do

    Insert

    7942 NS2 Up Flank
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    bp 422 A to T, bp 423 A to T, bp 663 inserted G. …

    Promoter

    Available since

    Aug. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pC0.175

    Plasmid

    #119643

    Purpose

    Level 0 Part. Insertion site

    Depositor

    Alistair McCormick

    Article

    Vasudevan et al Plant Physiol. 2019 May;180(1):39-55. do

    Insert

    7942 NS2 Up Flank
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    bp 422 A to T, bp 423 A to T, bp 663 inserted G. …

    Promoter

    Available since

    Aug. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge