We narrowed to 15,806 results for: nans
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Plasmid#172752PurposeBacterial expression of HIS-tagged anti-GFP nanobody LaG-27; HIS tag preceded by HRV 3C/PreScission cleavage site.DepositorInsertLaG-27
UseTags6xHIS and pelB leader for periplasmic secretionExpressionBacterialMutationPromoterT7Available sinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLenti-EF1a-MTS-FLAG-LOV*
Plasmid#202208PurposePlasmid for the generation of a lentiviral vector encoding the photoproximity labeling catalyst LOV* fused to the mitochondria targeting sequence (MTS) in mammalian cellsDepositorInsertCytochrome c oxidase subunit 4 isoform 1, mitochondrial, aa 1-24
UseLentiviralTagsFLAG and LOV*ExpressionMutationPromoterEf1aAvailable sinceAug. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-2Aneo
Plasmid#80032PurposeUsed to construct AAV-based targeting vectors that carry an IVS-2Aneo cassette for promoter-trappingDepositorTypeEmpty backboneUseAAV and Cre/Lox; A platform vector to construct t…TagsExpressionMutationPromoterAvailable sinceAug. 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
pUASTLOTattB_mCherry::Nrv1::vhhGFP4
Plasmid#163926PurposeExpression of GrabFP-B-intracellular under control of UAS or LOP enhancerDepositorInsertFusion of vhhGPF4 (intracellular) nanobody with Nrv1 protein and mCherry fluorophor
UseCre/LoxTagsmCherryExpressionInsectMutationPromoterAvailable sinceJan. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
Po_CyRPA-Cd4-bio-His
Plasmid#126829PurposeExpression of recombinant P. ovale protein in mammalian cellsDepositorInsertCyRPA
UseTagsratCd4(d3+4)-bio-6HisExpressionMammalianMutationPromoterCMVAvailable sinceMarch 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
Pm_CyRPA-Cd4-bio-His
Plasmid#126817PurposeExpression of recombinant P. malariae protein in mammalian cellsDepositorInsertCyRPA (PmUG01_05040800 Plasmodium malariae)
UseTagsratCd4(d3+4)-bio-6HisExpressionMammalianMutationPromoterCMVAvailable sinceMarch 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaG-24
Plasmid#172750PurposeBacterial expression of HIS-tagged anti-GFP nanobody LaG-24; HIS tag preceded by HRV 3C/PreScission cleavage site.DepositorInsertLaG-24
UseTags6xHIS and pelB leader for periplasmic secretionExpressionBacterialMutationPromoterT7Available sinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaG-9
Plasmid#172742PurposeBacterial expression of HIS-tagged anti-GFP nanobody LaG-9; HIS tag preceded by HRV 3C/PreScission cleavage site.DepositorInsertLaG-9
UseTags6xHIS and pelB leader for periplasmic secretionExpressionBacterialMutationPromoterT7Available sinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pNOC-CRISPR-35S
Plasmid#99861PurposeExpresses Cas9-Nlux and sgRNA. Contains CEN/ARS and 35 terminator. No selection marker.DepositorTypeEmpty backboneUseAlgae, nannochloropsis expressionTagsCas9-NluxExpressionMutationPromoterRibi promoterAvailable sinceFeb. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaG-8
Plasmid#172761PurposeBacterial expression of HIS-tagged anti-GFP nanobody LaG-8; HIS tag preceded by HRV 3C/PreScission cleavage site.DepositorInsertLaG-8
UseTags6xHIS and pelB leader for periplasmic secretionExpressionBacterialMutationPromoterT7Available sinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaG-5
Plasmid#172760PurposeBacterial expression of HIS-tagged anti-GFP nanobody LaG-5; HIS tag preceded by HRV 3C/PreScission cleavage site.DepositorInsertLaG-5
UseTags6xHIS and pelB leader for periplasmic secretionExpressionBacterialMutationPromoterT7Available sinceAug. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28a Gx-NL2
Plasmid#169107PurposeExpresses protein G fused to 2 copies of NanoLuc. The protein G domain contains an amber stop-codon at position 24 for incorporation of the unnatural amino acid para-benzoylphenylalanine.DepositorInsertprotein G - NanoLuc - NanoLuc
UseTagshis-tag and strep-tagExpressionBacterialMutationAla 24 stopPromoterT7Available sinceJune 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
Desmoglein2_tail-GST
Plasmid#36990DepositorInsertDsg2 (DSG2 Human)
UseTagsGSTExpressionBacterialMutationIntracellular domain of DSG2 (nucleotides 1911–35…PromoterAvailable sinceMay 23, 2012AvailabilityAcademic Institutions and Nonprofits only -
pUASTLOTattB_vhhGFP4::Vkg::mCherry
Plasmid#163929PurposeExpression of GrabFP-ECM under control of UAS or LOP enhancerDepositorInsertFusion of vhhGPF4 nanobody with Viking (Collagen IV) protein and mCherry fluorophor
UseCre/LoxTagsmCherryExpressionInsectMutationG1767DPromoterAvailable sinceFeb. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28a-T5-ASG_ClpXP
Plasmid#153299PurposeExpresses acoustic sensor genes or ClpXP-sensitive gas vesicles in E. coli Nissle 1917DepositorInsertASG
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterT5Available sinceSept. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLyGo-Ec-6
Plasmid#163132PurposepLyGo cloning vector for periplasmic expression in E. coli of a sequence of interest (LPMO). Vector encoding PelB signal peptide and the LyGo cassette (SapI-ccdB-SapI)DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterT7Available sinceJuly 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEF1a-T2-NLuc
Plasmid#135938PurposeExpresses NanoLuciferase targeted to the mitochondira in mammalian cells.DepositorInsertNanoLuciferase
UseTagsT2 mitochondrial localization sequenceExpressionMammalianMutationPromoterEF1aAvailable sinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLyGo-Kp-2
Plasmid#163144PurposepLyGo cloning vector for a sequence of interest (LPMO) in K. phaffii. Vector encoding the alfa-amylase signal peptide and the LyGo cassette (SapI-ccdB-SapI)DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionYeastMutationPromoterAOX1Available sinceMay 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaG-2
Plasmid#172739PurposeBacterial expression of HIS-tagged anti-GFP nanobody LaG-2; HIS tag preceded by HRV 3C/PreScission cleavage site.DepositorInsertLaG-2
UseTags6xHIS and pelB leader for periplasmic secretionExpressionBacterialMutationPromoterT7Available sinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTetR-BCD2-Ana GvpC
Plasmid#153298PurposeExpresses wild-type gas vesicle protein C (GvpC) from Anabaena flos-aquaeDepositorInsertGas vesicle protein C
UseTagsExpressionBacterialMutationPromoterpTetAvailable sinceSept. 21, 2020AvailabilityAcademic Institutions and Nonprofits only