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pDAS12489_PEAR-GFP_2in1_2.0

Plasmid

#177186

Purpose

PEAR-GFP3 plasmid with a pegRNA that targets its own plasmid

Depositor

Ervin Welker

Article

Simon et al Elife. 2022 Feb 23;11. pii: 69504. doi:

Inserts

EGFP split with an intron between amino acids 95-96
pegRNA targeting its own plasmid

Use

CRISPR

Expression

Mammalian

Mutation

disrupted 5' splice site

Promoter

CMV and U6

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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L4505 x6TRE2 (cJun2) Ben DsRed-Express2 in TUPV1

Plasmid

#244200

Purpose

Expression of a DsRed-Express2 reporter under a cJun-responsive promoter (cJun homodimer binding sites)

Depositor

Joshua Leonard

Article

Edelstein et al Nat Chem Biol. 2025 Aug 22:10.1038/s4158

Insert

cJun-responsive promoter (6 cJun homodimer binding sites + YB_TATA), dsRed-Express2 reporter

Use

Synthetic Biology

Expression

Mammalian

Available Since

Oct. 15, 2025

Availability

Academic Institutions and Nonprofits only

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pKanCMV-mClover3-mRuby3

Plasmid

#74252

Purpose

Mammalian expression of tandem fusion of mClover3-mRuby3

Depositor

Michael Lin

Article

Bajar et al Sci Rep. 2016 Feb 16;6:20889. doi: 10.10

Insert

mClover3-mRuby3

Expression

Mammalian

Promoter

CMV

Available Since

March 29, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 iTEV

Plasmid

#64277

Purpose

Expresses infrared fluorescent tobacco etch virus protease sensor (iTEV)

Depositor

Xiaokun Shu

Article

To et al Proc Natl Acad Sci U S A. 2015 Mar 17;11

Insert

Infrared fluorescent tobacco etch virus protease sensor (iTEV)

Expression

Mammalian

Promoter

CMV

Available Since

April 24, 2015

Availability

Academic Institutions and Nonprofits only

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pLenti-Cdc42-2G

Plasmid

#68813

Purpose

FRET-based biosensor reporting on endogenous Cdc42 activation (for Lentivirus production)

Depositor

Olivier Pertz

Article

Martin et al Sci Rep. 2016 Feb 25;6:21901. doi: 10.10

Insert

Cdc42 activation reporter

Use

Lentiviral

Tags

His and Myc

Expression

Mammalian

Promoter

CMV

Available Since

March 1, 2016

Availability

Academic Institutions and Nonprofits only

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pCAG-attBr-ZsGreen-attP

Plasmid

#51265

Purpose

Bxb1-responsive fluorescent reporter plasmid containing reverse complementary ZsGreen flanked by Bxb1 attB/P recognition sites in head-to-head orientation

Depositor

Pawel Pelczar

Article

Hermann et al Nucleic Acids Res. 2014 Jan 9.

Insert

attBr-ZsGreen-attP

Expression

Mammalian

Promoter

CAG

Available Since

March 4, 2014

Availability

Academic Institutions and Nonprofits only

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pRluc-LC3wt

Plasmid

#105002

Purpose

Mammalian expression of Rluc-LC3wt. Can be used for measuring autophagic flux.

Depositor

Marja Jããttelã

Article

Farkas et al Methods Enzymol. 2017;588:1-13. doi: 10.

Insert

LC3B (Map1lc3b Rat)

Use

Luciferase

Tags

Rluc with C124A substitution

Expression

Mammalian

Available Since

Jan. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pRRL-mU6-GFPGO2-IRES-mScarletI

Plasmid

#136896

Purpose

Lentivirus for base editing activatable GFP expression in mammalian cells. All-in-one vector with sgGO.

Depositor

Lukas Dow

Article

Katti et al Nucleic Acids Res. 2020 Feb 29. pii: 576

Insert

GFPGO2-IRES-mScarletI

Use

Lentiviral

Tags

2xNLS on GFP, 3xNLS on mScarlet

Mutation

ATG>ACG

Promoter

SFFV

Available Since

Feb. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pEF-BFP

Plasmid

#138272

Purpose

Expression of engineered BFP variant that undergoes conversion to GFP after targeted modification with a cytidine deaminase-based DNA base editor

Depositor

Xiao Wang

Article

Standage-Beier et al Nucleic Acids Res. 2019 Aug 20. pii: 555

Insert

BFP H66 PAM

Use

CRISPR

Expression

Mammalian

Mutation

H66, PAM motif

Promoter

Ef1a

Available Since

March 3, 2020

Availability

Academic Institutions and Nonprofits only

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pDAS12395_PEAR-GFP_2.0-preedited

Plasmid

#177185

Purpose

PEAR-GFP3 control plasmid with split EGFP and intact 5' splice site

Depositor

Ervin Welker

Article

Simon et al Elife. 2022 Feb 23;11. pii: 69504. doi:

Insert

EGFP split with an intron between amino acids 95-96

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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Salsa6f

Plasmid

#140188

Purpose

Salsa6f is a fluorescent ratiometric genetically encoded calcium indicator, (tdTomato linked to GCaMP6f by a V5 epitope tag)

Depositor

Michael Cahalan

Article

Dong et al eLife. 2017 Dec 14;6. pii: 32417. doi: 1

Insert

Salsa6f

Tags

tdTomato-v5-GCaMP6f

Expression

Mammalian

Promoter

CMV Immediate Early

Available Since

Nov. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pROSA26-PA

Plasmid

#21271

Depositor

Frank Costantini

Article

Srinivas et al BMC Dev Biol. 2001;1:4. doi: 10.1186/147

Insert

ROSA26 (Gt(ROSA)26Sor Mouse)

Use

Mouse Targeting

Available Since

Oct. 13, 2009

Availability

Academic Institutions and Nonprofits only

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PB-Tal1

Plasmid

#60670

Purpose

PiggyBac expression vector encoding murine Tal1 downstream of a doxycycline inducible promoter

Depositor

James Thomson

Article

Vereide et al Stem Cell Reports. 2014 Dec 9;3(6):1043-

Insert

Tal1 (Tal1 Mouse)

Use

Piggybac

Expression

Mammalian

Available Since

Sept. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-hRARβ.hRXRα

Plasmid

#135415

Purpose

Human Retinoic Acid Receptor-beta and Human Retinoid X Receptor-alpha in pcDNA3.1(+) plasmid expression vector

Depositor

Catharine Ross

Article

Zolfaghari et al Anal Biochem. 2019 Jul 15;577:98-109. do

Inserts

Human retinoic acid receptor-beta
Human retinoid X receptor-alpha

Expression

Mammalian

Promoter

CMV and SV40

Available Since

Jan. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pNC1124

Plasmid

#41560

Depositor

Beverly Errede

Article

Houser et al Yeast. 2012 Dec;29(12):519-30. doi: 10.1

Insert

Ura3-GAL1-UBIYdkGFP*-SpHis5-Tim9

Mutation

GFP mutations: Phe 64 to Leu, S65 to Thr, V163 t…

Available Since

March 1, 2013

Availability

Academic Institutions and Nonprofits only

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10XSTAT92E–luciferase

Plasmid

#37393

Depositor

Norbert Perrimon

Article

Baeg et al Genes Dev. 2005 Aug 15;19(16):1861-70. E

Insert

SOCS36E enhancer fragment (Socs36E Fly)

Use

Luciferase; Jak/stat reporter construct

Expression

Insect

Promoter

Hsp70

Available Since

Nov. 2, 2012

Availability

Academic Institutions and Nonprofits only

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pFast-FT-N1

Plasmid

#31910

Depositor

Vladislav Verkhusha

Article

Subach et al Nat Chem Biol. 2009 Feb;5(2):118-26. Epu

Insert

Fast-FT

Expression

Mammalian

Mutation

M18V/E34K/K69R/L84W/S149T/A224S compared to mCher…

Promoter

CMV

Available Since

Sept. 12, 2011

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-hRARα.hRXRα

Plasmid

#135411

Purpose

Human Retinoic Acid Receptor-alpha and Human Retinoid X Receptor-alpha in pcDNA3.1(+) plasmid expression vector

Depositor

Catharine Ross

Article

Zolfaghari et al Anal Biochem. 2019 Jul 15;577:98-109. do

Inserts

Human retinoic acid receptor-alpha
Human retinoid X receptor-alpha

Expression

Mammalian

Promoter

CMV and SV40

Available Since

Jan. 9, 2020

Availability

Academic Institutions and Nonprofits only

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XLone-ETV2

Plasmid

#135763

Purpose

expresses human ETV2 upon doxycycline treatment

Depositor

Xiaojun Lian

Article

Ream et al Stem Cell Reports. 2024 Mar 5:S2213-6711

Insert

ETV2 (ETV2 Human)

Expression

Mammalian

Mutation

none

Promoter

TRE3G

Available Since

March 25, 2024

Availability

Academic Institutions and Nonprofits only

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pSlow-FT-N1

Plasmid

#31912

Depositor

Vladislav Verkhusha

Article

Subach et al Nat Chem Biol. 2009 Feb;5(2):118-26. Epu

Insert

Slow-FT

Expression

Mammalian

Mutation

M18V/E30V/K69R/L84W/A179V compared to mCherry (bu…

Promoter

CMV

Available Since

Sept. 12, 2011

Availability

Academic Institutions and Nonprofits only

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