We narrowed to 2,878 results for: 623

1. pET29-hMIGA2(306-593)-6xhis

   Plasmid

   #192837

   Purpose

   Express codon-optimized human MIGA2 fragment 306-593 with a C-terminal 6xhis tag (lipid transfer construct) in bacteria

   Depositor

   Karin Reinisch

   Article

   Hong et al J Cell Biol. 2022 Dec 5;221(12):e2022070

   Insert

   Codon-optimized human Mitoguardin 2 (MIGA2 Human)
   

   Use

   Tags

   6xhis

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Dec. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. p3xFLAG CMV14 / BRAF-V600K

   Plasmid

   #131720

   Purpose

   Mammalian Expression of Flag tagged BRAF

   Depositor

   Jacques De Grève

   Article

   Noeparast et al Oncotarget. 2016 Aug 26;8(36):60094-6010

   Insert

   BRAF (BRAF Human)
   

   Use

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   V600K

   Promoter

   CMV

   Available since

   Dec. 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCIBN-LacI

   Plasmid

   #103814

   Purpose

   BLInCR 'Localizer' construct that marks lacO arrays and is targeted by a PHR-tagged effector upon illumination with blue light

   Depositor

   Karsten Rippe

   Article

   Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

   Insert

   CIBN-LacI (CIB1, lacI Mustard Weed, E. coli)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   LacI: C19T (silent, L7L), T264C (silent, A88A), C…

   Promoter

   CMV

   Available since

   Jan. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. p3xFLAG CMV14 / BRAF-D594E

   Plasmid

   #131714

   Purpose

   Mammalian Expression of Flag tagged BRAF

   Depositor

   Jacques De Grève

   Article

   Noeparast et al Oncotarget. 2016 Aug 26;8(36):60094-6010

   Insert

   BRAF (BRAF Human)
   

   Use

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   D594E

   Promoter

   CMV

   Available since

   July 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET28A-p53 (1-73) (P12A P13A)

   Plasmid

   #62066

   Purpose

   Expression of human p53 (residues 1-73) (P12A, P13A) in e. coli

   Depositor

   Gary Daughdrill

   Article

   Borcherds et al Nat Chem Biol. 2014 Dec;10(12):1000-2. d

   Insert

   p53 (TP53 Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Contains TAD residues 1-73; P12A, P13A

   Promoter

   T7

   Available since

   Feb. 23, 2015

   Availability

   Academic Institutions and Nonprofits only

6. pGEX Socs2-SH2

   Plasmid

   #46505

   Depositor

   Bruce Mayer

   Article

   Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

   Insert

   Socs2 (SOCS2 Human)
   

   Use

   Tags

   GST and PreScission

   Expression

   Bacterial

   Mutation

   contains amino acids 28-162

   Promoter

   Tac

   Available since

   July 12, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. p3xFLAG CMV14 / BRAF-G466V

   Plasmid

   #131722

   Purpose

   Mammalian Expression of Flag tagged BRAF

   Depositor

   Jacques De Grève

   Article

   Noeparast et al Oncotarget. 2016 Aug 26;8(36):60094-6010

   Insert

   BRAF (BRAF Human)
   

   Use

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   G466V

   Promoter

   CMV

   Available since

   June 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. AL05_pDonor.DMD

   Plasmid

   #100284

   Purpose

   DMD intron 43-targeting plasmid

   Depositor

   Manuel Goncalves

   Article

   Chen et al Nat Commun. 2017 Sep 22;8(1):657. doi: 1

   Insert

   EGFP expression unit (DMD Human, Synthetic)
   

   Use

   CRISPR and Synthetic Biology; Human targeting

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 22, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. AC62_pDonor.DMD.TS.DR

   Plasmid

   #100288

   Purpose

   DMD intron 43-targeting plasmid

   Depositor

   Manuel Goncalves

   Article

   Chen et al Nat Commun. 2017 Sep 22;8(1):657. doi: 1

   Insert

   EGFP expression unit (DMD Human, Synthetic)
   

   Use

   CRISPR and Synthetic Biology; Human targeting

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 22, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. TFORF3146

    Plasmid

    #144622

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    HSF1 (HSF1 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    May 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCS2+ E4F1 degron(220-242)-GFP-IRES-mCherry

    Plasmid

    #231007

    Purpose

    Protein stability reporter construct for E4F1 consisting of aa 220-242 for transient overexpression in mammalian cells.

    Depositor

    Michael Rape

    Article

    Manford et al Cell. 2021 Oct 14;184(21):5375-5390.e16.

    Insert

    E4F1 aa220-242 (E4F1 Human)
    

    Use

    Tags

    AcGFP1

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. (attB) tdMCP-tagged BRaf

    Plasmid

    #226165

    Purpose

    Used to integrate 3xFlag-tdMCP-BRaf without MS2-circRNA barcdes into landing pad cell lines containing a single promoter.

    Depositor

    Dustin Maly

    Article

    Simon et al Nat Methods. 2024 Nov;21(11):2094-2106.

    Insert

    3xFlag-tdMCP-BRaf (BRAF Human)
    

    Use

    Bxb1 attb, no promoter

    Tags

    3xFlag-tdMCP and IRES-mCherry-P2A-PuroR

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pHAGE-EF1aL-E690Kmut_ABCA3-GFP

    Plasmid

    #188549

    Purpose

    Lentiviral vector allowing for EF1aL-driven constitutive expression of E690K mutant human ABCA3:GFP fusion protein

    Depositor

    Darrell Kotton

    Article

    Sun et al J Clin Invest. 2024 Jan 16;134(2):e16427

    Insert

    ABCA3:GFP (ABCA3 Human)
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mutation

    amino acid E690->K690 (ABCA3 gene)

    Promoter

    EF1aL

    Available since

    Oct. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pHAGE-EF1aL-W308Rmut_ABCA3-GFP

    Plasmid

    #188550

    Purpose

    Lentiviral vector allowing for EF1aL-driven constitutive expression of W308R mutant human ABCA3:GFP fusion protein

    Depositor

    Darrell Kotton

    Article

    Sun et al J Clin Invest. 2024 Jan 16;134(2):e16427

    Insert

    ABCA3 (ABCA3 Human)
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mutation

    amino acid W308->R308 (ABCA3 gene)

    Promoter

    EF1aL

    Available since

    Oct. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pJExpress401-KDAC7 CD

    Plasmid

    #224341

    Purpose

    Expresses human KDAC7 (HDAC7) catalytic domain in E. coli

    Depositor

    Terry Watt

    Article

    Toro et al Protein Expr Purif. 2018 Jan;141:1-6. do

    Insert

    KDAC7 (HDAC7 Human)
    

    Use

    Tags

    TEV-cleavable His6

    Expression

    Bacterial

    Mutation

    Only includes residues 521-942 (catalytic domain).

    Promoter

    T5

    Available since

    Oct. 22, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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16. pFastBacI-KDAC7 CD

    Plasmid

    #224345

    Purpose

    Expresses human KDAC7 (HDAC7) catalytic domain in insect cells

    Depositor

    Terry Watt

    Article

    Toro et al Protein Expr Purif. 2018 Jan;141:1-6. do

    Insert

    KDAC7 (HDAC7 Human)
    

    Use

    Tags

    TEV-cleavable His6

    Expression

    Insect

    Mutation

    Only includes residues 521-942 (catalytic domain).

    Promoter

    Polyhedrin

    Available since

    Oct. 22, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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17. PGK_ACE2-ECD_Notch-Gal4VP64 (pZYW037)

    Plasmid

    #194226

    Purpose

    Lentiviral expression vector expressing ACE2-ECD SynNotch and mCherry transduction marker

    Depositor

    Hana El-Samad

    Article

    Weinberg et al bioRxiv 2021.04.20.440678

    Insert

    PGK-ACE2-SynNotch-T2A-mCherry (ACE2 Synthetic)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    PGK

    Available since

    Sept. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pTFG001-ACE2(WT)-HA

    Plasmid

    #191569

    Purpose

    ACE2 insert in a 2nd generation lentiviral transfer plasmid (pGIPZ) to generate ACE2(WT)-expressing cells. Codon-optimized for human expression. Insert contains C-terminal HA tag.

    Depositor

    Joshua Leonard

    Article

    Gunnels et al Small. 2022 May;18(19):e2200125. doi: 10

    Insert

    Angiotensin Converting Enzyme II (ACE2 Human)
    

    Use

    Lentiviral and RNAi

    Tags

    HA tag

    Expression

    Mammalian

    Mutation

    Codon optimized for expression in human cells

    Promoter

    CMV

    Available since

    Aug. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDONR201-Eps8L2_494-546

    Plasmid

    #196531

    Purpose

    Entry vector for Gateway with Eps8L2_494-546

    Depositor

    Edgar Gomes

    Article

    Calero-Cuenca et al Curr Biol. 2021 Apr 12;31(7):1521-1530.e

    Insert

    Eps8L2_494-546 (EPS8L2 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    CMV promoter; T7 promoter

    Available since

    July 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pDONR201-Eps8L2_367-496

    Plasmid

    #196530

    Purpose

    Entry vector for Gateway with Eps8L2_367-496

    Depositor

    Edgar Gomes

    Article

    Calero-Cuenca et al Curr Biol. 2021 Apr 12;31(7):1521-1530.e

    Insert

    Eps8L2_367-496 (EPS8L2 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    CMV promoter; T7 promoter

    Available since

    June 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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