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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 2,251 results for: EYFP

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Showing: 2001 - 2020 of 2251 results

  2. pcDNA3.1 Flag YFP hNKCC1 HA-ECL4 (NT935)

    Plasmid
    #49065
    Purpose
    Expresses human NKCC1 mutant with HA tag inserted into extracellular loop #4 and N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.
    Depositor
    Biff Forbush
    Publication
    Somasekharan et al PLoS One. 2013 Dec 5;8(12):e82060. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    human NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
    Tags
    3x Flag and mVenus
    Expression
    Mammalian
    Mutation
    2xHA epitope in ECL4 inserted at aa574 in hNKCC1 …
    Promoter
    CMV
    Available since
    Nov. 12, 2013
    Availability
    Academic Institutions and Nonprofits only
    49065-plasmid-map-sequence-id-72190

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  6. pZyxin-mMaple2

    Plasmid
    #101150
    Purpose
    Fluorescent protein for superresolution imaging and a focal adhesion protein for binding substrate
    Depositor
    Xiaowei Zhuang
    Publication
    Wang et al Proc Natl Acad Sci U S A. 2014 Jun 10;11
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    human codon optimized mMaple2 fused to zyxin
    Expression
    Mammalian
    Promoter
    cmv
    Available since
    March 19, 2018
    Availability
    Academic Institutions and Nonprofits only
    101150-plasmid-map-sequence-id-205974

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  9. C1-F-tractin(delta1-6)-mCitrine

    Plasmid
    #155219
    Purpose
    F-actin bidning mutant of F-tractin
    Depositor
    Tobias Meyer
    Publication
    Bisaria et al Science. 2020 Jun 12;368(6496):1205-1210
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    F-tractin (aa 9-52 of rat ITPKA) fused to mCitrine (Itpka Rat)
    Tags
    mCitrine
    Expression
    Mammalian
    Mutation
    Deletion of first 6 AA from F-tractin (9-14 from …
    Available since
    Sept. 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    155219-plasmid-map-sequence-id-310147

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  14. pLV_PP1-mCitrine

    Plasmid
    #172472
    Purpose
    Mammalian expression of a human codon-optimized version of the optogenetic GPCR parapinopsina (PP1) from zebrafish fused to mCitrine. Also contains an N-terminal prolactin signal sequence.
    Depositor
    Sean R Collins
    Publication
    Bell et al Nat Commun. 2021 Nov 16;12(1):6148. doi:
    Insert
    prolactinSS-PP1-mCitrine (parapinopsina Zebrafish)
    Use
    Lentiviral
    Tags
    mCitrine and prolactin signal sequence (cleaved p…
    Expression
    Mammalian
    Promoter
    EF1alpha
    Available since
    Sept. 15, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    172472-plasmid-map-sequence-id-342800

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  15. FKBP-Stx3-mCitrine

    Plasmid
    #92428
    Purpose
    Expression of syntaxin-3 N-terminally conjugated to FKBP and C-terminally conjugated to mCitrine.
    Depositor
    Geert van den Bogaart
    Publication
    Verboogen et al Elife. 2017 May 19;6. pii: e23525. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Stx3 (Stx3 Rat)
    Tags
    FKBP (FK506 binding protein 12); allows for heter…
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    July 12, 2017
    Availability
    Academic Institutions and Nonprofits only
    92428-plasmid-map-sequence-id-182510

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  16. Epac-S-H30

    Plasmid
    #170337
    Purpose
    CFP(UST)_EPACdDEPCD_Y(UST) biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells.
    Depositor
    Kees Jalink
    Publication
    Ponsioen et al EMBO Rep. 2004 Dec . 5(12):1176-80.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    CFP(UST)_EPACdDEPCD_Y(UST) (RAPGEF3 Human)
    Expression
    Mammalian
    Mutation
    Deletion of DEP domain, Catalytically dead T781A …
    Promoter
    CMV
    Available since
    Oct. 5, 2021
    Availability
    Academic Institutions and Nonprofits only
    170337-plasmid-map-sequence-id-344639

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  17. pB80-hsKIF5B(1-560)Rigor-L-mVenus-L-SspB(micro)

    Plasmid
    #193721
    Purpose
    Optogenetic coupling to dimeric human kinesin-1 (KIF5B aa1-807) via SSPB(micro) for anterograde transport
    Depositor
    Lukas Kapitein
    Publication
    Burute et al Sci Adv. 2022 Nov 4;8(44):eabo2343. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    hsKIF5B(1-560)Rigor-L-mVenus-L-SspB(micro) (KIF5B Human, Synthetic)
    Tags
    mVenus-SSPB(micro)
    Expression
    Mammalian
    Mutation
    hsKIF5B(aa1-560), RIGOR: G234A; mVenus: Met1Del, …
    Promoter
    Chicken beta-actin
    Available since
    March 2, 2023
    Availability
    Academic Institutions and Nonprofits only
    193721-plasmid-map-sequence-id-383728

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  18. pcDNA3.1 Flag YFP hNKCC1 HA-ECL3 (NT933)

    Plasmid
    #49064
    Purpose
    Expresses human NKCC1 mutant with HA tag inserted into extracellular loop #3 and N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.
    Depositor
    Biff Forbush
    Publication
    Somasekharan et al PLoS One. 2013 Dec 5;8(12):e82060. doi:
    Insert
    human NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
    Tags
    3x Flag and mVenus
    Expression
    Mammalian
    Mutation
    2xHA epitope in ECL3 inserted at aa460 in hNKCC1 …
    Promoter
    CMV
    Available since
    Nov. 12, 2013
    Availability
    Academic Institutions and Nonprofits only
    49064-plasmid-map-sequence-id-72188

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  20. pcDNA3.1 Flag YFP hNKCC1 N-terminus T,S mutations (NT51)

    Plasmid
    #49061
    Purpose
    Expresses human NKCC1 mutated Ser and Thr residues in the N-terminus and an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.
    Depositor
    Biff Forbush
    Publication
    Somasekharan et al PLoS One. 2013 Dec 5;8(12):e82060. doi:
    Insert
    human NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
    Tags
    3x Flag and mVenus
    Expression
    Mammalian
    Mutation
    S150N, S155Q, S170Q, T177A, S183R, S193E, T203A, …
    Promoter
    CMV
    Available since
    Nov. 19, 2013
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    49061-plasmid-map-sequence-id-72183

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Showing: 2001 - 2020 of 2251 results