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PX458_GABPA_2

Plasmid

#64255

Purpose

Expresses gRNA against human GABPA along with Cas9 with 2A GFP

Depositor

Eric Mendenhall, Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

gRNA

Use

CRISPR

Available Since

July 8, 2015

Availability

Academic Institutions and Nonprofits only

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pHES820

Plasmid

#89190

Purpose

Expresses YFP under control of GAL1 promoter.

Depositor

Hana El-Samad

Article

Aranda-Diaz et al ACS Synth Biol. 2016 Dec 27. doi: 10.102

Insert

pGAL1-YFP

Expression

Yeast

Promoter

GAL1

Available Since

Oct. 20, 2017

Availability

Academic Institutions and Nonprofits only

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PX458_GABPA_1

Plasmid

#64254

Purpose

Expresses gRNA against human GABPA along with Cas9 with 2A GFP

Depositor

Eric Mendenhall, Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

gRNA

Use

CRISPR

Available Since

July 8, 2015

Availability

Academic Institutions and Nonprofits only

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pCS6-mPSD95a-TS2-HA

Plasmid

#42240

Depositor

Roger Tsien

Article

Butko et al Nat Neurosci. 2012 Nov;15(12):1742-51. d

Insert

PSD95

Tags

TS2-HA

Expression

Mammalian

Available Since

March 13, 2013

Availability

Academic Institutions and Nonprofits only

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pHES854

Plasmid

#89197

Purpose

Expresses mKate2 under control of TDH3 constitutive promoter.

Depositor

Hana El-Samad

Article

Aranda-Diaz et al ACS Synth Biol. 2016 Dec 27. doi: 10.102

Insert

pTDH3-mKate2

Expression

Yeast

Promoter

TDH3

Available Since

Dec. 7, 2017

Availability

Academic Institutions and Nonprofits only

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PX458_ATF1_1

Plasmid

#64690

Purpose

Expresses gRNA against human ATF1 along with Cas9 with 2A GFP

Depositor

Eric Mendenhall, Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

gRNA

Use

CRISPR

Available Since

July 8, 2015

Availability

Academic Institutions and Nonprofits only

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EcAvs2

Plasmid

#188789

Purpose

Avs2 from Escherichia coli NCTC9087

Depositor

Feng Zhang

Article

Gao et al Science. 2022 Aug 12;377(6607):eabm4096.

Insert

EcAvs2

Expression

Bacterial

Available Since

Aug. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pLV VEET-GFP

Plasmid

#188706

Purpose

Lentivirus transfer plasmid encoding VEET-GFP

Depositor

David Tobin

Article

Brewer et al Cell Rep. 2022 Dec 13;41(11):111817. doi

Insert

VEET-GFP

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 13, 2022

Availability

Academic Institutions and Nonprofits only

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AlACR1_EYFP_pcDNA3.1

Plasmid

#161022

Purpose

Expresses strongly red-shifted AlACR1 in mammalian cells

Depositor

John Spudich

Article

Govorunova et al Proc Natl Acad Sci U S A. 2020 Sep 15;11

Insert

AlACR1

Tags

EYFP

Expression

Mammalian

Available Since

Nov. 17, 2020

Availability

Academic Institutions and Nonprofits only

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KpAvs4

Plasmid

#188796

Purpose

Avs4 from Klebsiella pneumoniae UNMC_8078

Depositor

Feng Zhang

Article

Gao et al Science. 2022 Aug 12;377(6607):eabm4096.

Insert

KpAvs4

Expression

Bacterial

Available Since

Aug. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pFETCh_SIX4_iso1

Plasmid

#128238

Purpose

Donor vector for 3' FLAG tag of human SIX4_iso1

Depositor

Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

SIX4_iso1 Homology arms

Use

CRISPR

Tags

3XFLAG-P2A-NeoR

Available Since

Nov. 18, 2019

Availability

Academic Institutions and Nonprofits only

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6xHis-MBP-huPcCas12a

Plasmid

#174681

Purpose

Expresses 6xHis-MBP tagged PcCas12a

Depositor

Piyush Jain

Article

Nguyen et al medRxiv 2021.07.21

Insert

huPcCas12a

Use

CRISPR and Synthetic Biology

Tags

6xHis, MBP, TEV and NLS, 6xHis, 3xHA

Expression

Bacterial

Promoter

T7

Available Since

Oct. 25, 2021

Availability

Academic Institutions and Nonprofits only

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6xHis-MBP-huPdCas12a

Plasmid

#174682

Purpose

Expresses 6xHis-MBP tagged PdCas12a

Depositor

Piyush Jain

Article

Nguyen et al medRxiv 2021.07.21

Insert

huPdCas12a

Use

CRISPR and Synthetic Biology

Tags

6xHis, MBP, TEV and NLS, 6xHis, 3xHA

Expression

Bacterial

Promoter

T7

Available Since

Oct. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pXcX-G4BS-3.6TPH-EGFP

Plasmid

#32572

Depositor

Sergey Kasparov

Article

Benzekhroufa et al BMC Biotechnol. 2009 Mar 19;9:23.

Insert

EGFP

Use

Adenoviral

Expression

Mammalian

Promoter

3.6TPH

Available Since

Feb. 2, 2012

Availability

Academic Institutions and Nonprofits only

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pNR186

Plasmid

#79552

Purpose

Output plasmid: contains 5-state register that regulates expression of GFP. Doesn't behave as expected.

Depositor

Timothy Lu

Article

Roquet et al Science. 2016 Jul 22;353(6297):aad8559.

Insert

GFPmut3b

Expression

Bacterial

Available Since

July 22, 2016

Availability

Academic Institutions and Nonprofits only

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BASIC_2_Kan-MB1

Plasmid

#68136

Purpose

storage vector for BASIC part: Kan-MB1

Depositor

Geoffrey Baldwin

Article

Storch et al ACS Synth Biol. 2015 Apr 16.

Insert

Kan-MB1

Expression

Bacterial

Available Since

Aug. 26, 2015

Availability

Academic Institutions and Nonprofits only

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pMPO 54

Plasmid

#69865

Purpose

Expression vectors for the salycilate cascade expression system (Pnah/NahR-Psal/XylS2).ApR, expression vector with rrnBT1T2-Pm-MCSII, M13 replication origin

Depositor

Eduardo Santero

Article

Medina et al PLoS One. 2011;6(8):e23055. doi: 10.1371

Type

Empty backbone

Expression

Bacterial

Available Since

Dec. 9, 2015

Availability

Academic Institutions and Nonprofits only

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pLenti-EBFP-VAV1-W495L

Plasmid

#242839

Purpose

Encodes human VAV1 and N-terminal EBFP2 tag

Depositor

Cheng-han Yu

Article

Qi et al Cell Mol Life Sci. 2025 Mar 24;82(1):127

Insert

VAV1 (VAV1 Human)

Use

Lentiviral

Tags

EBFP2

Expression

Mammalian

Mutation

W495L

Available Since

Oct. 17, 2025

Availability

Academic Institutions and Nonprofits only

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DE_Thr6XHisFLAG

Plasmid

#139949

Purpose

Protein tags

Depositor

Raffaela Abbriano

Article

Jaramillo-Madrid et al Pharmaceuticals (Basel). 2020 Dec 21;13(

Insert

Thrombin6XHisFLAGStop

Use

Synthetic Biology

Available Since

Oct. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pBR322-sgRNA-pvc18-20

Plasmid

#243747

Purpose

PVC cargo/regulatory region - deleted toxin cargos (Pdp1/Pnf) - vegfa sgRNA

Depositor

Feng Zhang

Article

Kreitz et al Nat Biotechnol. 2025 Aug 12. doi: 10.103

Insert

vegfa sgRNA-pvc18-20

Expression

Bacterial

Available Since

Sept. 24, 2025

Availability

Academic Institutions and Nonprofits only

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