We narrowed to 3,906 results for: SPL

1. pET:ACAT:GFP

   Plasmid

   #60740

   Purpose

   A modified pET28 driving expression of GFP, biobrick part Bba_E0040. No LacO site downstream of PT7. T7 promoter has base pairs 'ACAT' between -11 and -8.

   Depositor

   Matthew Bennett

   Article

   Shis et al Proc Natl Acad Sci U S A. 2013 Mar 26;11

   Insert

   GFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 5, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. 3158 pSFFV-neo Bad cDNA

   Plasmid

   #8753

   Depositor

   Stanley Korsmeyer

   Article

   Yang et al Cell 1995 Jan 27;80(2):285-91.

   Insert

   BAD (Bad Mouse)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 6, 2005

   Availability

   Academic Institutions and Nonprofits only

3. pM215A

   Plasmid

   #173217

   Purpose

   Expresses ScFV-GCN4-sFGFP10M2-GB1

   Depositor

   Hajin Kim

   Article

   Chaudhary et al Genome Res. 2020 Sep;30(9):1306-1316. do

   Insert

   ScFV-GCN4-sfGFP10M2-GB1
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pM214

   Plasmid

   #173216

   Purpose

   Expresses MCP-mCherry-GFP11-GB1

   Depositor

   Hajin Kim

   Article

   Chaudhary et al Genome Res. 2020 Sep;30(9):1306-1316. do

   Insert

   MCP-mCherry-GFP11-GB1
   

   Use

   CRISPR and Lentiviral

   Tags

   HA and mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 31, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEBB-II-I-deltaNLS1-EGFP

   Plasmid

   #22150

   Depositor

   Ananda Roy

   Article

   Cheriyath et al J Biol Chem. 2000 Aug 25. 275(34):26300-

   Insert

   TFII-I (GTF2I Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Deletion of amino acids 297-304

   Promoter

   Available since

   Nov. 13, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEIIIA

   Plasmid

   #14059

   Depositor

   Richard Hynes

   Article

   Paul et al J Biol Chem. 1986 Sep 15. 261(26):12258-

   Insert

   Rat Fibronectin EIIIA (Fn1 Rat)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   EcoRI linkers added to TaqI to HinfI fragment

   Promoter

   Available since

   March 2, 2007

   Availability

   Academic Institutions and Nonprofits only

7. pET:CCCT:GFP

   Plasmid

   #60738

   Purpose

   A modified pET28 driving expression of GFP, biobrick part Bba_E0040. No LacO site downstream of PT7. T7 promoter has base pairs 'CCCT' between -11 and -8.

   Depositor

   Matthew Bennett

   Article

   Shis et al Proc Natl Acad Sci U S A. 2013 Mar 26;11

   Insert

   GFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 18, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSLQ8454 pHR-hU6-LbCpf1 crRNA BB-EF1a-Puro-WPRE

   Plasmid

   #140087

   Purpose

   Backbone for cloning U6 driven LbCpf1 (Cas12a) crRNA, with EF1a driven puromycin resistance

   Depositor

   Stanley Qi

   Article

   Kempton et al Mol Cell. 2020 Jan 30. pii: S1097-2765(2

   Insert

   LbCpf1 (LbCas12a) crRNA backbone
   

   Use

   Lentiviral

   Tags

   EF1a-Puro

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   May 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. p3x-Flag Gli3 30R3-1

   Plasmid

   #120304

   Purpose

   Expresses human protein Gli containing a 4HT-inducible intein evolved to operate at 30C

   Depositor

   David Liu

   Article

   Peck et al Chem Biol. 2011 May 27;18(5):619-30. doi

   Insert

   30R3-1, Gli3
   

   Use

   Synthetic Biology

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pTEI135

    Plasmid

    #126073

    Purpose

    Vector for expression of mitochondria targeted YFP1-10 (T203Y) in plant cell

    Depositor

    Ralf-Bernd Klösgen

    Article

    Sharma et al J Cell Sci. 2019 Jun 3;132(11). pii: jcs

    Insert

    mitochondria Rieske iron sulphur protein (1-100 aa)
    

    Use

    Binary

    Tags

    YFP1-10

    Expression

    Plant

    Mutation

    Promoter

    Available since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pTEI165

    Plasmid

    #126074

    Purpose

    Vector for expression of plastid targeted improved YFP1-10 (T65G,T205S, T203Y) in plant cell

    Depositor

    Ralf-Bernd Klösgen

    Article

    Sharma et al J Cell Sci. 2019 Jun 3;132(11). pii: jcs

    Insert

    Chloroplastic FNR transit peptide
    

    Use

    Binary

    Tags

    YFP1-10 (improved)

    Expression

    Plant

    Mutation

    Promoter

    Available since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTEI166

    Plasmid

    #126075

    Purpose

    Vector for expression of mitochondria targeted improved YFP1-10 (T65G, T205S, T203Y) in plant cell

    Depositor

    Ralf-Bernd Klösgen

    Article

    Sharma et al J Cell Sci. 2019 Jun 3;132(11). pii: jcs

    Insert

    mitochondria Rieske iron sulphur protein (1-100 aa)
    

    Use

    Binary

    Tags

    YFP1-10 (improved)

    Expression

    Plant

    Mutation

    Promoter

    Available since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pTEI128

    Plasmid

    #126209

    Purpose

    Module 1 vector of PlaMiNGo toolkit for cloning of candidate gene at N-terminus of GFP11x3 and expression in plant cell

    Depositor

    Ralf-Bernd Klösgen

    Article

    Sharma et al J Cell Sci. 2019 Jun 3;132(11). pii: jcs

    Inserts

    ccdB Cassette (BsaI)
    GFP11x3 (5 aa linker)
    

    Use

    Binary, module 1

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcFN-C (chick fibronectin)

    Plasmid

    #14063

    Depositor

    Richard Hynes

    Article

    Norton et al Mol Cell Biol. 1987 Dec . 7(12):4297-307

    Insert

    Chicken Fibronectin (FN1 Chicken)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    DraI - EcoRI fragment inserted in HincII-EcoRI si…

    Promoter

    Available since

    March 2, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. 3156 pBS Bad cDNA

    Plasmid

    #8752

    Depositor

    Stanley Korsmeyer

    Article

    Yang et al Cell 1995 Jan 27;80(2):285-91.

    Insert

    BAD (Bad Mouse)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 2, 2005

    Availability

    Academic Institutions and Nonprofits only

16. pNMHCII-C2-GFP

    Plasmid

    #46043

    Depositor

    Robert Adelstein

    Article

    Jana et al J Biol Chem. 2009 Apr 24;284(17):11563-7

    Insert

    nonmuscle myosin heavy chain II-C, isoform C2 (Myh14 Mouse)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV immediate early

    Available since

    July 31, 2013

    Availability

    Academic Institutions and Nonprofits only

17. pGADT7 Hsh155 Y292C

    Plasmid

    #87291

    Purpose

    Encodes Y2H Gal4 activation domain for Hsh155 Y292C

    Depositor

    Aaron Hoskins

    Article

    Carrocci et al Nucleic Acids Res. 2017 Jan 6. pii: gkw1

    Insert

    HSH155 (HSH155 Budding Yeast)
    

    Use

    Tags

    GAL4 AD and HA

    Expression

    Bacterial and Yeast

    Mutation

    Y292C

    Promoter

    Available since

    July 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. GST-PRMT9 G260E mutant

    Plasmid

    #67610

    Purpose

    bacterial expression of GST-PRMT9 G260E Double E loop mutant

    Depositor

    Steven Clarke

    Article

    Hadjikyriacou et al J Biol Chem. 2015 May 15. pii: jbc.M115.

    Insert

    PRMT9 (PRMT9 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    G260E double E loop mutant

    Promoter

    tac

    Available since

    June 27, 2016

    Availability

    Academic Institutions and Nonprofits only

19. GST-PRMT9 D258G mutant

    Plasmid

    #67609

    Purpose

    bacterial expression of GST-PRMT9 D258G Double E loop mutant

    Depositor

    Steven Clarke

    Article

    Hadjikyriacou et al J Biol Chem. 2015 May 15. pii: jbc.M115.

    Insert

    PRMT9 (PRMT9 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    D258G double E loop mutant

    Promoter

    tac

    Available since

    June 24, 2016

    Availability

    Academic Institutions and Nonprofits only

20. PLL7.0 tgRFP LOV

    Plasmid

    #114463

    Purpose

    Mammalian expression of tgRFP labelled LOV2

    Depositor

    Andrew Woolley

    Article

    Reis et al ACS Synth Biol. 2018 Oct 19;7(10):2355-2

    Insert

    LOV2
    

    Use

    Tags

    tgRFP

    Expression

    Mammalian

    Mutation

    G401A(G528A), N411E(N538E) and V289I (V416I)

    Promoter

    CMV

    Available since

    Dec. 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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