We narrowed to 25,701 results for: nes

Showing: 2001 - 2020 of 25701 results

Results per page:

Sort results by:

pMD19T-psba1-TetR-PL22-dCas9-SpR

Plasmid

#73223

Purpose

Contains dCas9 from S. pyogenes under aTc inducible promoter. Suicide vector inserts into psba1 site of Synechocystis. Carries spectinomycin resist. Recommend E. coli Copy cutter for propogation.

Depositor

Paul Hudson

Article

Yao et al ACS Synth Biol. 2015 Dec 28.

Insert

dCas9 from S. pyogenes

Tags

c-myc

Expression

Bacterial

Mutation

Silent mutation at bp 1341 A->C to remove an E…

Promoter

PL22

Available Since

March 15, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
FUGW-Golgi-TurboID

Plasmid

#200964

Purpose

3rd gen lentiviral plasmid for expressing golgi localized TurboID with EGFP

Depositor

Keriann Backus

Article

Yan et al Cell Chem Biol. 2023 Jul 20;30(7):811-82

Insert

Golgi-TurboID

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Available Since

Aug. 18, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1-TYMS

Plasmid

#100545

Purpose

mammalian expression of thymidylate synthetase

Depositor

Yuh-Shan Jou

Article

Yeh et al Oncotarget. 2017 Sep 8;8(44):77734-77751

Insert

thymidylate synthetase (TYMS Human)

Expression

Mammalian

Available Since

Sept. 12, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pK7WGF2::hCas9

Plasmid

#46965

Purpose

Expresses the human codon usage Cas9 nuclease (Mali et al. Science 339, 823-826, 2013) with an N-terminal GFP tag from the 35S promoter in the plant tissue

Depositor

Sophien Kamoun

Article

Nekrasov et al Nat Biotechnol. 2013 Aug 8;31(8):691-3.

Insert

hCas9

Use

CRISPR; Plant expression

Tags

eGFP

Promoter

35S

Available Since

Aug. 16, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
Stat3 Flag pRc/CMV

Plasmid

#8707

Depositor

Jim Darnell

Article

Horvath et al Genes Dev 1995 Apr 15;9(8):984-94.

Insert

Stat3 (Stat3 Mouse)

Tags

Flag

Expression

Mammalian

Mutation

*see comments

Available Since

Sept. 26, 2005

Availability

Academic Institutions and Nonprofits only

Enlarge
pEF1a-Ronin-FLAG-IRES-Neo

Plasmid

#28020

Depositor

Thomas Zwaka

Article

Dejosez et al Genes Dev. 2010 Jul 15. 24(14):1479-84.

Insert

Ronin (THAP11 Human)

Tags

FLAG

Expression

Mammalian

Available Since

May 17, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
lentiCRISPR-v2-HygR-mCherry

Plasmid

#167189

Purpose

Lentiviral expression of sgRNA with hygromycin resistance gene and mCherry

Depositor

Yin Shen

Article

Ren et al Sci Adv. 2021 Sep 17;7(38):eabi4360. doi

Insert

Hygromycin B phosphotransferase and mCherry

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Oct. 25, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
MSCV-PIG-Cre

Plasmid

#50935

Purpose

retroviral expression of Cre recombinase

Depositor

Pier Pandolfi

Article

Chen et al Nature. 2005 Aug 4;436(7051):725-30.

Insert

Cre recombinase

Use

Cre/Lox and Retroviral

Tags

NLS

Expression

Mammalian

Available Since

Jan. 16, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
PECAM1-3'gRNA

Plasmid

#235606

Purpose

gRNA targeting PECAM1 3' terminal for CRISPR-Cas9-mediated knock-in

Depositor

Kai Wang

Article

Zhao et al Angiogenesis. 2025 Jan 3;28(1):10. doi:

Insert

PECAM1 gRNA (PECAM1 Human)

Use

CRISPR

Promoter

U6

Available Since

June 17, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pDisplay-UDP1.0-IRES-mCherry-CAAX

Plasmid

#220850

Purpose

CMV promoter drives the expression of an extracellular UDP sensor (UDP1.0) and a membrane localized mCherry

Depositor

Yulong Li

Article

Umpierre et al Neuron. 2024 Apr 10:S0896-6273(24)00195-

Insert

UDP1.0

Expression

Mammalian

Promoter

CMV

Available Since

July 16, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pETM6-At4CL-PhCHS-CmCHI (C5 mutant, pFlavo-opt)

Plasmid

#73404

Purpose

Expresses three enzymes to convert phenylpropanoic acids to flavanones in vivo

Depositor

Mattheos Koffas

Article

Jones et al Metab Eng. 2016 Feb 6;35:55-63. doi: 10.

Inserts

At4CL
PhCHS
CmCHI

Expression

Bacterial

Promoter

T7

Available Since

March 1, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pGL3-24p3/LCN2-Luc

Plasmid

#25463

Depositor

Sarah Gaffen

Article

Shen et al J Leukoc Biol. 2005 Mar . 77(3):388-99.

Insert

24p3/lipocalin 2 proximal promoter (Lcn2 Mouse)

Use

Luciferase

Mutation

none

Available Since

June 25, 2010

Availability

Academic Institutions and Nonprofits only
pDestattB_ubi:EGFP (pCM272)

Plasmid

#68339

Purpose

Multisite Gateway destination vector for phiC31-mediated transgenesis in zebrafish, harbors ubiquitin promoter (ubi)-driven EGFP.

Depositor

Christian Mosimann, Leonard Zon

Article

Mosimann et al Dev Dyn. 2013 Aug;242(8):949-63. doi: 10

Insert

EGFP

Use

Zebrafish transgenesis

Promoter

ubi

Available Since

Aug. 13, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pEC3006 (pSpy1C-PZn_ffluc)

Plasmid

#218515

Purpose

replicative E. coli-S. pyogenes shuttle plasmid for zinc-inducible expression of the Firefly luciferase in S. pyogenes

Depositor

Emmanuelle Charpentier

Article

Lautenschlager et al Front Bioeng Biotechnol. 2024 Jun 7;12:1

Insert

ffluc

Use

Luciferase

Expression

Bacterial

Mutation

deletion of Plac_mrfp cassette

Promoter

PZn (PczcD)

Available Since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
PresentER-MSIIFFLPL (GFP)

Plasmid

#102946

Purpose

PresentER minigene encoding H2-Kb ligand MSIIFFLPL (GFP)

Depositor

David Scheinberg

Article

Gejman et al Elife. 2018 Nov 30;7. pii: 41090. doi: 1

Insert

MSIIFFLPL (Serpinf1 Mouse, Synthetic)

Use

Retroviral

Expression

Mammalian

Promoter

LTR

Available Since

April 17, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
MSCVhygro-F-Ezh2-F667I

Plasmid

#24927

Depositor

Kai Ge

Article

Wang et al Proc Natl Acad Sci U S A. 2010 Apr 20. 1

Insert

Ezh2 (EZH2 Mouse)

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Mutation

Phenylanaline(F)667 mutated to Isoleucine(I)

Available Since

July 15, 2010

Availability

Academic Institutions and Nonprofits only
pRN3P-HA-Suv4-20h2

Plasmid

#86691

Purpose

For transcription of of Suv4-20h2 mRNA preceded by an HA-tag in 5'

Depositor

Maria-Elena Torres-Padilla

Article

Eid et al Genes Dev. 2016 Nov 15;30(22):2513-2526.

Insert

histone-lysine N-methyltransferase KMT5C (Kmt5c Mouse)

Tags

HA

Promoter

T3

Available Since

March 28, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pU6-SAG1-DHFR

Plasmid

#80322

Purpose

Encodes a CRISPR sgRNA that allows for mutation of the surface antigen SAG1 in Toxoplasma gondii and confers resistance to pyrimethamine

Depositor

Sebastian Lourido

Article

Sidik et al Cell. 2016 Sep 8;166(6):1423-1435.e12. d

Insert

SAG1 sgRNA

Use

CRISPR

Promoter

U6

Available Since

Sept. 12, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pBa.GFP-KIF13B

Plasmid

#134626

Purpose

Mammalian expression of mouse KIF13B 442-1843

Depositor

Marvin Bentley

Article

Yang et al Traffic. 2019 Aug 28. doi: 10.1111/tra.1

Insert

KIF13B (Kif13b Mouse)

Tags

eGFP

Expression

Mammalian

Mutation

WT

Promoter

Chicken Beta actin

Available Since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.2/V5 hsa-mir-150

Plasmid

#26310

Depositor

David Bartel

Article

Chiang et al Genes Dev. 2010 May 15. 24(10):992-1009.

Insert

hsa-mir-150 (MIR150 Human)

Expression

Mammalian

Available Since

Sept. 29, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge