161,697 results

1. pTXB1-nbOcIgG-Tn5

   Plasmid

   #184285

   Purpose

   Anti-rabbit IgG Fc specific nanobody-Tn5 for NTT-seq (multiplexed CUT&Tag)

   Depositor

   Ivan Raimondi, New York Genome Center

   Article

   Stuart et al Nat Biotechnol. 2022 Dec 19. doi: 10.103

   Insert

   nbOcIgG-Tn5
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. TFORF1844

   Plasmid

   #144313

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   RB1 (RB1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Aug. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBOB-CAG-SARS-CoV2-Spike-HA

   Plasmid

   #141347

   Purpose

   3. Generation lentiviral vector expressing the codon optimized SARS-CoV2 Spike Glycoprotein ORF with a C-terminal HA tag generated by Junko Ogawa & Gerald M Pao

   Depositor

   Gerald Pao

   Article

   Ogawa et al bioRxiv. 2020 Jul 22. doi: 10.1101/2020.

   Insert

   SARS-CoV2 Spike Glycoprotein (S SARS-CoV2 hCoV19_USA EPI_ISL_414366 (GISAID))
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mutation

   resynthesized with human codon optimization nucle…

   Promoter

   CAG

   Available since

   May 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGEX-2T PTP-1B

   Plasmid

   #8602

   Depositor

   Ben Neel

   Insert

   PTP-1B (PTPN1 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 4, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCMV enAsCas12f-YHAM-sgRNA_v7

   Plasmid

   #208356

   Purpose

   Expression of enAsCas12f-YHAM and sgRNA_v7 in mammalian cells

   Depositor

   Atsushi Hoshino

   Article

   Hino et al Cell. 2023 Sep 22:S0092-8674(23)00963-7.

   Insert

   enAsCas12f-YHAM
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   F48Y/S188H/V232A/E316M

   Promoter

   CMV

   Available since

   Oct. 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. Flag-SKP1-T131A

   Plasmid

   #213482

   Purpose

   Expresses human SKP1 T131A mutant in mammalian cells

   Depositor

   Michele Pagano

   Article

   Li et al Sci Adv. 2023 Oct 13;9(41):eadh1134. doi

   Insert

   human Flag tagged SKP1-T131A (SKP1 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. PKD1 gRNA (BRDN0001149544)

   Plasmid

   #76841

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human PKD1

   Depositor

   David Root, John Doench

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   PKD1 (PKD1 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pFlagCMV2-EFP

   Plasmid

   #12449

   Depositor

   Dong-Er Zhang

   Article

   Zou et al J Biol Chem. 2006 Feb 17. 281(7):3989-94

   Insert

   EFP (TRIM25 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 4, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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9. p236

   Plasmid

   #186182

   Purpose

   Shotgun Metagenomic Spike-in

   Depositor

   Karsten Zengler

   Article

   Zaramela et al mSystems. 2022 Dec 20;7(6):e0044722. doi

   Insert

   synDNA 236
   

   Use

   Synthetic Biology; Cloning

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. TurboID-V5_pRS415

    Plasmid

    #107167

    Purpose

    expresses V5-tagged TurboID in the yeast cytosol

    Depositor

    Alice Ting

    Article

    Branon et al Nat Biotechnol. 2018 Aug 20. pii: nbt.42

    Insert

    TurboID (BirA mutant)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Q65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…

    Promoter

    Available since

    May 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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