We narrowed to 13,516 results for: Arch

1. shMEK1/2 v2 puro

   Plasmid

   #72568

   Purpose

   Hairpin targeting MEK1 and MEK2 (murine).

   Depositor

   Kevin Janes

   Article

   Jensen et al Cell Syst. 2016 Feb 24;2(2):112-121.

   Insert

   MEK1/2 (Map2k1, Map2k2 Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 12, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pB-RTP1S-Integration

   Plasmid

   #129458

   Purpose

   piggyBac transposon vector that Inducibly (Tet-On) expresses RTP1S

   Depositor

   Sriram Kosuri

   Article

   Jones et al Cell Syst. 2019 Mar 27;8(3):254-260.e6.

   Insert

   RTP1 (Rtp1 Mouse)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Removal of the first 36 amino acids of the protein

   Promoter

   TRE (Tet-On)

   Available since

   Aug. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCEP4-HA-Flag-natT1R2 a22-end_I132S-S212N

   Plasmid

   #113952

   Purpose

   mammalian expression plasmid for FLAG-tagged human T1R2 with signal peptide of influenza hemagglutinin; expression-enhanced variant

   Depositor

   Erik Procko

   Article

   Park et al J Biol Chem. 2019 Feb 5. pii: RA118.0061

   Insert

   T1R2 (TAS1R2 Human)
   

   Use

   Tags

   FLAG and Signal/leader sequence from influenza he…

   Expression

   Mammalian

   Mutation

   delete N-terminal 21 residues, I132S and S212N su…

   Promoter

   cmv

   Available since

   Feb. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. [pSK483] pLJC5 Flag-Depdc5(P)

   Plasmid

   #109342

   Purpose

   Lenti-viral expression of Flag-tagged Depdc5 mutant P

   Depositor

   Kuang Shen, David Sabatini

   Article

   Shen et al Nature. 2018 Apr 5;556(7699):64-69. doi:

   Insert

   Depdc5 (DEPDC5 Human)
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mutation

   YDLLP(775-779)GSGSG

   Promoter

   Available since

   July 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.1(+) Laccase2 MCS-HIPK3 Exon

   Plasmid

   #69899

   Purpose

   Expresses the human HIPK3 exon 2 circular RNA in mammalian cells

   Depositor

   Jeremy Wilusz

   Article

   Kramer et al Genes Dev. 2015 Oct 8.

   Insert

   HIPK3 (stw Fly, Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Expresses aa1-194 of HIPK3

   Promoter

   CMV

   Available since

   Nov. 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pENTR CMVie-Lifeact-iRFP670 L1-R5

   Plasmid

   #84390

   Purpose

   Multisite Gateway entry vector for expression of iRFP670-tagged Lifeact in cells including neurons

   Depositor

   Ghassan Mouneimne

   Article

   Padilla-Rodriguez et al Nat Commun. 2018 Jul 30;9(1):2980. doi:

   Insert

   Lifeact
   

   Use

   Gateway

   Tags

   iRFP670

   Expression

   Mammalian

   Mutation

   Promoter

   CMV immediate early

   Available since

   Jan. 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. [pSK496] pLJC5 HA-Depdc5(AB)

   Plasmid

   #109346

   Purpose

   Lenti-viral expression of HA-tagged Depdc5 mutant AB

   Depositor

   Kuang Shen, David Sabatini

   Article

   Shen et al Nature. 2018 Apr 5;556(7699):64-69. doi:

   Insert

   Depdc5 (DEPDC5 Human)
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mutation

   DIYGD(185-189)GSGSG;EQPLH(371-375)GSGSG

   Promoter

   Available since

   July 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. NET97 pmRFP-N2 (641)

   Plasmid

   #61994

   Purpose

   mammalian expression of nuclear envelope transmembrane protein

   Depositor

   Eric Schirmer

   Article

   Korfali et al Mol Cell Proteomics. 2010 Dec;9(12):2571

   Insert

   AADACL1 (NCEH1 Human)
   

   Use

   Tags

   mRFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 28, 2015

   Availability

   Academic Institutions and Nonprofits only

9. pCEP4-HA-Flag-natT1R2 a22-end_S212N

   Plasmid

   #113951

   Purpose

   mammalian expression plasmid for FLAG-tagged human T1R2 with signal peptide of influenza hemagglutinin; expression-enhanced variant

   Depositor

   Erik Procko

   Article

   Park et al J Biol Chem. 2019 Feb 5. pii: RA118.0061

   Insert

   T1R2 (TAS1R2 Human)
   

   Use

   Tags

   FLAG and Signal/leader sequence from influenza he…

   Expression

   Mammalian

   Mutation

   delete N-terminal 21 residues, S212N substitution

   Promoter

   CMV

   Available since

   Feb. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pJT131_GalL_cCDA1-NGBE3

    Plasmid

    #145095

    Purpose

    Expressing base editor cCDA1-NGBE3 in yeast cells

    Depositor

    Ralph Bock

    Article

    Tan et al Nat Commun. 2020 Jan 31;11(1):629. doi:

    Insert

    cCDA1-NGBE3
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    spCas9(D10A/L1111R/D1135V/G1218R/E1219F/A1322R/R1…

    Promoter

    GalL

    Available since

    July 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. [pSK497] pLJC5 Flag-Depdc5(AB)

    Plasmid

    #109341

    Purpose

    Lenti-viral expression of Flag-tagged Depdc5 mutant AB

    Depositor

    Kuang Shen, David Sabatini

    Article

    Shen et al Nature. 2018 Apr 5;556(7699):64-69. doi:

    Insert

    Depdc5 (DEPDC5 Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mutation

    DIYGD(185-189)GSGSG;EQPLH(371-375)GSGSG

    Promoter

    Available since

    July 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. tdEos-Farnesyl-5

    Plasmid

    #57613

    Purpose

    Localization: Plasma Membrane, Excitation: 505 / 569, Emission: 516 / 581

    Depositor

    Michael Davidson

    Article

    Kanchanawong et al Nature. 2010 Nov 25;468(7323):580-4. doi

    Insert

    Farnesyl (HRAS Human)
    

    Use

    Tags

    tdEos

    Expression

    Mammalian

    Mutation

    encodes final 20 aa of NM_001130442.1

    Promoter

    CMV

    Available since

    Jan. 16, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

13. STT3A pmRFP (663)

    Plasmid

    #61997

    Purpose

    mammalian expression of nuclear envelope transmembrane protein

    Depositor

    Eric Schirmer

    Article

    Korfali et al Mol Cell Proteomics. 2010 Dec;9(12):2571

    Insert

    STT3A (STT3A Human)
    

    Use

    Tags

    mRFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

14. pcDNA3.1(+) Laccase2 MCS-ZKSCAN1 548-847

    Plasmid

    #69894

    Purpose

    Expresses a miniature version of the ZKSCAN1 circular RNA in mammalian cells

    Depositor

    Jeremy Wilusz

    Article

    Kramer et al Genes Dev. 2015 Oct 8.

    Insert

    ZKSCAN1 (stw, ZKSCAN1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. His-MBP-NRBF2 (1-159)

    Plasmid

    #99331

    Purpose

    Mammalian expression of the monomeric form of NRBF2 that binds with the PI3K C1 complex

    Depositor

    James Hurley

    Article

    Young et al Proc Natl Acad Sci U S A. 2016 Jul 19;11

    Insert

    Nuclear receptor-binding factor 2 (NRBF2 Human)
    

    Use

    Tags

    6xHis-MBP-TEV

    Expression

    Bacterial

    Mutation

    AA1-159 are present

    Promoter

    T7

    Available since

    Aug. 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. Rescue Septin 11 mRNA

    Plasmid

    #122025

    Purpose

    Rescue mRNA for Septin 11 Knock Down with sh2 shRNA. Splice variant II (EU711415) with 5 silent mutations at SEPT11 sh2 shRNA target site.

    Depositor

    Angel de Blas

    Article

    Li et al J Biol Chem. 2009 Jun 19;284(25):17253-6

    Insert

    SEPT11 (Sept11 Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Silent mutations are in 5 consecutive codons (CUG…

    Promoter

    Available since

    Feb. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. Hy_pMT Laccase2 MCS-ZKSCAN1 548-1417 (No intron)

    Plasmid

    #69887

    Purpose

    Expresses a miniature version of the ZKSCAN1 circular RNA in Drosophila

    Depositor

    Jeremy Wilusz

    Article

    Kramer et al Genes Dev. 2015 Oct 8.

    Insert

    ZKSCAN1 (stw, ZKSCAN1 Human)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    F169L in ZKSCAN1

    Promoter

    Metallothionein Promoter (pMT)

    Available since

    Nov. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3.1(+) Laccase2 MCS-ZKSCAN1 548-1047

    Plasmid

    #69896

    Purpose

    Expresses a miniature version of the ZKSCAN1 circular RNA in mammalian cells

    Depositor

    Jeremy Wilusz

    Article

    Kramer et al Genes Dev. 2015 Oct 8.

    Insert

    ZKSCAN1 (stw, ZKSCAN1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. shMEK1/2 neo

    Plasmid

    #72569

    Purpose

    Hairpin targeting MEK1 and MEK2 (murine).

    Depositor

    Kevin Janes

    Article

    Jensen et al Cell Syst. 2016 Feb 24;2(2):112-121.

    Insert

    MEK1/2 (Map2k1, Map2k2 Mouse)
    

    Use

    Lentiviral

    Tags

    None

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Hy_pMT Laccase2 MCS-ZKSCAN1 548-1047

    Plasmid

    #69886

    Purpose

    Expresses a miniature version of the ZKSCAN1 circular RNA in Drosophila

    Depositor

    Jeremy Wilusz

    Article

    Kramer et al Genes Dev. 2015 Oct 8.

    Insert

    ZKSCAN1 (stw, ZKSCAN1 Human)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Metallothionein Promoter (pMT)

    Available since

    Nov. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge