We narrowed to 12,494 results for: LEA
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pcDNA3.1-V5-hTEV
Plasmid#65800Purposeexpresses human codon-optimised TEV proteaseDepositorInsertV5-human TEV
TagsV5ExpressionMammalianMutationcodon-optimised to express in mammalian cellsPromoterCMVAvailable sinceJune 8, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pGBW-m4046785
Plasmid#145684PurposeBacterial Expression plasmid for SARS-CoV-2 N (nucleocapsid)DepositorInsertSARS-CoV-2 N (nucleocapsid) (N Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
TagsCleavable TEV;StrepIIExpressionBacterialMutationEscherichia coli recode 1PromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available sinceApril 29, 2020AvailabilityIndustry, Academic Institutions, and NonprofitsCited by -
pDEST14-DogCatcher
Plasmid#171772PurposeExpresses DogCatcher protein in bacterial cytoplasmDepositorInsertDogCatcher
TagsHis6 and TEV cleavage siteExpressionBacterialPromoterT7Available sinceJuly 29, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pET28a MISTIC
Plasmid#85999Purposebacterial expression of transmembrane proteins using N-terminal MISTIC and C-terminal HIS6 tagDepositorInsertN/A
TagsHIS6 and MISTICExpressionBacterialMutationcodon optimzed for bacterial expressionPromoterT7Available sinceApril 11, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pINDUCER20-α-Syn
Plasmid#92200PurposeRetroviral overexpression vector (doxycycline-inducible) for α-SynDepositorAvailable sinceJune 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pX330S-6
Plasmid#58782PurposeExpresses Cas9 nuclease and gRNADepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInserthumanized S. pyogenes Cas9 nuclease
UseCRISPRTags3xFLAGExpressionMammalianPromoterCBhAvailable sinceNov. 7, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA-RanWT-mRFP1-polyA
Plasmid#59750PurposeExpress wild-type form of Ran-mRFP1 in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertRan-mRFP1
ExpressionMammalianPromoterCMV and T7Available sinceSept. 16, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pBUN6I11
Plasmid#50580PurposeCRISPR/Cas based plant genome editing and gene regulation; expresses dCas9-KRAB, gRNA scaffold for insertion of target sequence (OsU3 promoter), Bar resistanceDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertsdCas9-KRAB
gRNA scaffold
UseCRISPR; Plant expressionTags3xFLAG, HA, and NLSMutationdCas9 that is defective in DNA cleavage; the maiz…PromoterOsU3p and Ubi1pAvailable sinceDec. 11, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pEGFP-PLAA
Plasmid#85669PurposeExpression of PLAA with N-terminal GFP tagDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertPLAA (PLAA Human)
TagsGFPExpressionMammalianPromoterCMVAvailable sinceJan. 31, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pET21-pelB-LaM-4
Plasmid#172770PurposeBacterial expression of anti-mCherry nanobody LaM-4, with pelB leader and C-term free cysteine and 6xHIS tag.DepositorInsertLaM-4
Tags6xHIS and pelB leader for periplasmic secretionExpressionBacterialPromoterT7Available sinceAug. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLL3.7-EF-EYFP-YAP1_WT-PolyA
Plasmid#112284PurposeFluorescently labels WT human YAP1-2gamma isoform (504 aa)DepositorAvailable sinceMay 13, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pRFP-NLS
Plasmid#105296PurposeTo visualize nuclear dynamics in living cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertRac1 (RAC1 Human)
TagsRFPExpressionMammalianMutationaa encoding 172-192Available sinceFeb. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
pICE-HA-MRE11-H63D
Plasmid#82036PurposePlasmid for constitutive or doxycycline-inducible expression of H63D exonuclease-dead mutant of human MRE11. Confers resistance to puromycin. Use T-REx cells for doxycycline-inducible expression.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertMRE11 (MRE11 Human)
TagsHAExpressionMammalianMutationExonuclease-dead mutant: H63D MRE11PromoterCMV-tetAvailable sinceSept. 20, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA3.1(+)-HLA-Flag-natT1R3
Plasmid#113950Purposemammalian expression plasmid for FLAG-tagged human T1R3 with signal peptide of HLA class I histocompatibility antigen A-2 alpha chainDepositorInsertT1R3 (TAS1R3 Human)
TagsFLAG and Signal/leader sequence from HLA class I …ExpressionMammalianMutationresidues 21-852Available sinceFeb. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCRISPR-S12
Plasmid#84031PurposeTo episomally express codon optimized Cas9 and chimeric guide RNADepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertshSpCas9
eGFP
Tags3x FLAGExpressionMammalianPromoterCMV and CMV (downstream of F2A self-cleaving pept…Available sinceAug. 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
mEmerald-Vinculin-N-21
Plasmid#54304PurposeLocalization: Focal Adhesions, Excitation: 487, Emission: 509DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertVinculin (VCL Human)
TagsmEmeraldExpressionMammalianPromoterCMVAvailable sinceJune 16, 2014AvailabilityIndustry, Academic Institutions, and NonprofitsCited by -
pAAV-EF1a-mCherry-IRES-Dre
Plasmid#55633PurposeExpresses Dre in Mammalian CellsDepositorInsertsmCherry
Dre
UseAAVExpressionMammalianPromoterEf1a and Ef1a/IRESAvailable sinceJuly 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
pECIA2
Plasmid#47032PurposeThis is a Gateway destination vector for secreted expression in Drosophila cell culture by induction via CuSO4. Inserts will be C-terminally tagged with Fc, V5 and hexahistidine tags.DepositorTypeEmpty backboneUseSecreted expression in drosophila cultureTagsFc(human IgG1), HRV 3C Protease Cleavage site, He…ExpressionInsectPromoterMetallothionein (Copper-Inducible)Available sinceAug. 30, 2013AvailabilityAcademic Institutions and Nonprofits only -
pcDNA1 Flag ATXN1[2Q]
Plasmid#33235DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertAtaxin-1 (ATXN1 Human)
TagsFlagExpressionMammalianMutationinsert contains 2Q (2 glutamines) and there isno …PromoterCMVAvailable sinceApril 17, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by
