We narrowed to 3,959 results for: LYC

1. pET28 mOct4-POU

   Plasmid

   #206393

   Purpose

   Expresses the DNA binding domain of the Oct4 with a 6xHis tag for protein purification

   Depositor

   Ralf Jauch

   Article

   Tan et al Mol Biol Evol. 2021 Jun 25;38(7):2854-28

   Insert

   Oct4 (Pou5f1 Mouse)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   POU DNA binding domain only

   Promoter

   Available since

   Sept. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pKG-GW1-hCIT

   Plasmid

   #203166

   Purpose

   Yeast expression vector for human DHDDS

   Depositor

   William Sessa

   Article

   Park et al Cell Metab. 2014 Sep 2;20(3):448-57. doi

   Insert

   DHDDS (DHDDS Human)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   TDH3

   Available since

   Aug. 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEGFPC2-gephyrin S268E/270E

   Plasmid

   #199609

   Purpose

   Encodes N-terminal eGFP-tagged P1-gephyrin S268/270E for expression in mammalian cells.

   Depositor

   Shiva Tyagarajan

   Article

   Tyagarajan et al J Biol Chem. 2013 Apr 5;288(14):9634-47.

   Insert

   Gephyrin (Gphn Rat)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   S268E/S270E

   Promoter

   CMV

   Available since

   Aug. 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pQCXIH FRB Ubiqutin-BFP

   Plasmid

   #202426

   Purpose

   TagBFP FKBP-rapamycin binding (FRB) dimerization domain fused to ubiquitin

   Depositor

   David Bryant

   Article

   Roman-Fernandez et al Sci Adv. 2023 Feb 3;9(5):eabq1858. doi:

   Insert

   FKBP-rapamycin binding (FRB) dimerization domain
   

   Use

   Lentiviral

   Tags

   Ubiquitin, TagBFP

   Expression

   Mutation

   Promoter

   Available since

   July 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. LENTICRISPR-ENO1_sgRNA1

   Plasmid

   #201592

   Purpose

   CRISPR/Cas9-mediated gene knock-out

   Depositor

   Vamsi Mootha, Alexis Jourdain

   Article

   Jourdain et al Mol Cell. 2021 May 6;81(9):1905-1919.e12

   Insert

   ENO1 (ENO1 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. LENTICRISPR-ENO1_sgRNA2

   Plasmid

   #201593

   Purpose

   CRISPR/Cas9-mediated gene knock-out

   Depositor

   Vamsi Mootha, Alexis Jourdain

   Article

   Jourdain et al Mol Cell. 2021 May 6;81(9):1905-1919.e12

   Insert

   ENO1 (ENO1 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. LENTICRISPR-HNRNPD_sgRNA2

   Plasmid

   #201599

   Purpose

   CRISPR/Cas9-mediated gene knock-out

   Depositor

   Vamsi Mootha, Alexis Jourdain

   Article

   Jourdain et al Mol Cell. 2021 May 6;81(9):1905-1919.e12

   Insert

   HNRNPD (HNRNPD Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. LENTICRISPR-LUC7L_sgRNA1

   Plasmid

   #201600

   Purpose

   CRISPR/Cas9-mediated gene knock-out

   Depositor

   Vamsi Mootha, Alexis Jourdain

   Article

   Jourdain et al Mol Cell. 2021 May 6;81(9):1905-1919.e12

   Insert

   LUC7L (LUC7L Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. LENTICRISPR-LUC7L_sgRNA2

   Plasmid

   #201601

   Purpose

   CRISPR/Cas9-mediated gene knock-out

   Depositor

   Vamsi Mootha, Alexis Jourdain

   Article

   Jourdain et al Mol Cell. 2021 May 6;81(9):1905-1919.e12

   Insert

   LUC7L (LUC7L Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. LENTICRISPR-LUC7L3_sgRNA1

    Plasmid

    #201604

    Purpose

    CRISPR/Cas9-mediated gene knock-out

    Depositor

    Vamsi Mootha, Alexis Jourdain

    Article

    Jourdain et al Mol Cell. 2021 May 6;81(9):1905-1919.e12

    Insert

    LUC7L3 (LUC7L3 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. LENTICRISPR-LUC7L3_sgRNA2

    Plasmid

    #201605

    Purpose

    CRISPR/Cas9-mediated gene knock-out

    Depositor

    Vamsi Mootha, Alexis Jourdain

    Article

    Jourdain et al Mol Cell. 2021 May 6;81(9):1905-1919.e12

    Insert

    LUC7L3 (LUC7L3 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLJM1-Lamp1 FLAG

    Plasmid

    #199750

    Purpose

    Lentiviral construct to stably express Lamp1

    Depositor

    Roberto Zoncu

    Article

    Shin et al Science. 2022 Sep 16;377(6612):1290-1298

    Insert

    Lamp1 (Lamp1 Rat)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    May 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pRK5-myc Metap2

    Plasmid

    #199685

    Purpose

    Transient expression of Metap2

    Depositor

    Roberto Zoncu

    Article

    Shin et al Science. 2022 Sep 16;377(6612):1290-1298

    Insert

    METAP2 (METAP2 Human)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. GB1-PRD(703-868, P801G, G808C, C813S)-Strep

    Plasmid

    #169750

    Purpose

    Codon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-ALIX(703-868, P801G, G808C, C813S)-Strep.

    Depositor

    Lalit Deshmukh

    Article

    Elias et al J Biol Chem. 2021 Oct 21:101328. doi: 10

    Insert

    GB1-His6-TEV-ALIX(703-868, P801G, G808C, C813S)-Strep (PDCD6IP Human)
    

    Use

    Tags

    GB1-His6-TEV and Strep tag (WSHPQFEK)

    Expression

    Bacterial

    Mutation

    Proline 801 to Glycine, Glycine 808 to Cysteine, …

    Promoter

    Available since

    May 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSpCas9n(BB)-2A-Puro (PX462) V2.0 Stx5DL-B2

    Plasmid

    #165083

    Purpose

    gRNA 2 of pair B for Stx5L-specific knockout

    Depositor

    Geert van den Bogaart

    Article

    Linders et al Nat Commun. 2021 Oct 28;12(1):6227. doi:

    Insert

    hSpCas9n-2A-Puro
    

    Use

    CRISPR

    Tags

    2A-Puro and 3XFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSpCas9n(BB)-2A-Puro (PX462) V2.0 Stx5DL-C1

    Plasmid

    #165085

    Purpose

    gRNA 1 of pair C for Stx5L-specific knockout

    Depositor

    Geert van den Bogaart

    Article

    Linders et al Nat Commun. 2021 Oct 28;12(1):6227. doi:

    Insert

    hSpCas9n-2A-Puro
    

    Use

    CRISPR

    Tags

    2A-Puro and 3XFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSpCas9n(BB)-2A-Puro (PX462) V2.0 Stx5DL-C2

    Plasmid

    #165086

    Purpose

    gRNA 2 of pair C for Stx5L-specific knockout

    Depositor

    Geert van den Bogaart

    Article

    Linders et al Nat Commun. 2021 Oct 28;12(1):6227. doi:

    Insert

    hSpCas9n-2A-Puro
    

    Use

    CRISPR

    Tags

    2A-Puro and 3XFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. ST-Tstem(5A)-GFP

    Plasmid

    #162502

    Purpose

    Expresses a ST6GAL1 mutant with a GFP tag in its C-terminus in mammalian cells. The mutated stem region of Tac is inserted within ST.

    Depositor

    Lei Lu

    Article

    Sun et al J Biol Chem. 2020 Aug 21. pii: RA120.014

    Insert

    ST6Gal1 mutant (the mutated Tac stem region is inserted within ST) (ST6GAL1, IL2RA Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    The mutated Tac stem region is inserted within ST…

    Promoter

    Available since

    Feb. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. EcNGsC-DelFSH(307-309)-pKK223

    Plasmid

    #131377

    Purpose

    Low level expression of E.coli G.stearothermophillus MutY chimera protein with deletion of residues FSH 307-309 in Gs MutY.

    Depositor

    Martin Horvath

    Article

    Russelburg et al ACS Chem Biol. 2019 Dec 27. doi: 10.1021

    Insert

    EcNGsC MutY chimera DelFSH(307-309)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Fusion of two MutY genes. Residues 1-225 are from…

    Promoter

    tac

    Available since

    Jan. 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. EcNGsC-S308P-H309D-pKK223

    Plasmid

    #131378

    Purpose

    Low level expression of E.coli G.stearothermophillus MutY chimera protein with amino acid changes S308P and H309D in Gs MutY.

    Depositor

    Martin Horvath

    Article

    Russelburg et al ACS Chem Biol. 2019 Dec 27. doi: 10.1021

    Insert

    EcNGsC MutY chimera S308P H309D
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Fusion of two MutY genes. Residues 1-225 are from…

    Promoter

    tac

    Available since

    Jan. 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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