Addgene

We narrowed to 5,144 results for: PEP

Showing: 2021 - 2040 of 5144 results

Results per page:

Sort results by:

pET23PI-Sce ESARC D218A

Plasmid

#195608

Purpose

Expresses PI-SceI (VDE) homing endonuclease/intein from the T7 promoter that contains the D218A active site mutation

Depositor

Frederick Gimble

Article

Gimble et al J Biol Chem. 1995 Mar 17;270(11):5849-56

Insert

PI-SceI

Use

Tags

Expression

Bacterial

Mutation

T928C, T929C, A942C

Promoter

T7

Available since

Feb. 1, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pET23PI-Sce ESARC

Plasmid

#195607

Purpose

Expresses PI-SceI (VDE) homing endonuclease/intein from the T7 promoter

Depositor

Frederick Gimble

Article

Gimble et al J Biol Chem. 1995 Mar 17;270(11):5849-56

Insert

PI-SceI

Use

Tags

Expression

Bacterial

Mutation

C552T,C554A, A557T,G584T, C588A, G878T, C881T, C9…

Promoter

T7

Available since

Jan. 31, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
VAMP8

Plasmid

#186689

Purpose

Expresses VAMP8 in E. Coli

Depositor

Axel Brunger

Article

Lai et al Nature. 2022 Mar;603(7903):949-956. doi:

Insert

VAMP8

Use

Tags

3xFLAG, GST, TEV, and Thrombin

Expression

Bacterial

Mutation

Promoter

T7

Available since

Oct. 4, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
p-preSufI(IAC)

Plasmid

#168516

Purpose

Modified form of pre-SufI in pET25b. With C-terminal HHHHHHC (6xHisC) extension and mutations C17I and C295A.

Depositor

Siegfried Musser

Article

Bageshwar et al PLoS One. 2021 Sep 9;16(9):e0256715. doi

Insert

pre-SufI(IAC)

Use

Tags

6xHisC tag and HSV tag

Expression

Bacterial

Mutation

C17I and C295A

Promoter

T7

Available since

Sept. 16, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
Stx3

Plasmid

#186685

Purpose

Expresses Syntaxin 3 in E. Coli

Depositor

Axel Brunger

Article

Lai et al Nature. 2022 Mar;603(7903):949-956. doi:

Insert

Syntaxin 3

Use

Tags

6 his tag

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 8, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
1056D

Plasmid

#183132

Purpose

Plasmid supports expression of 2 gRNAs targeting D.suzukii bTub, Opie-mVenus tagged, and can be integrated with pBac.

Depositor

Omar Akbari

Article

Kandul et al GEN Biotechnology (2022) 1:4, 372-385

Insert

U6.3-gRNAs[bTub]

Use

CRISPR

Tags

Expression

Insect

Mutation

Promoter

Available since

June 10, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
1056E

Plasmid

#183133

Purpose

Plasmid supports expression of 2 gRNAs targeting D.suzukii dsx, Opie-mVenus tagged, and can be integrated with pBac.

Depositor

Omar Akbari

Article

Kandul et al GEN Biotechnology (2022) 1:4, 372-385

Insert

U6.3-gRNAs[dsx]

Use

CRISPR

Tags

Expression

Insect

Mutation

Promoter

Available since

June 10, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
1056F

Plasmid

#183134

Purpose

Plasmid supports expression of 2 gRNAs targeting D.suzukii tra, Opie-mVenus tagged, and can be integrated with pBac.

Depositor

Omar Akbari

Article

Kandul et al GEN Biotechnology (2022) 1:4, 372-385

Insert

U6.3-gRNAs[tra]

Use

CRISPR

Tags

Expression

Insect

Mutation

Promoter

Available since

June 10, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
TDRD3:ABC006-C02:C220502

Plasmid

#183982

Purpose

Baculovirus expression for structure determination. May not contain entire coding region of gene.

Depositor

Cheryl Arrowsmith

Insert

TDRD3-anti-TDRD3 FAB (TDRD3 Human)

Use

Tags

anti-TDRD3 FAB HC: N-terminal STII leader peptid…

Expression

Bacterial

Mutation

Promoter

Available since

June 6, 2022

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
Mito-CoilE-mEm

Plasmid

#181994

Purpose

CoilE genetically encoded tag on Mito-mEmerald, to be used with CoilR labeled with a reporter

Depositor

Kimberly Beatty

Article

Doh et al Proc Natl Acad Sci U S A. 2018 Dec 18;11

Insert

Mito-CoilE-mEm

Use

Tags

mEmerald

Expression

Mammalian

Mutation

Promoter

Available since

March 24, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
TOMM20-CoilE-mCherry

Plasmid

#181995

Purpose

CoilE genetically encoded tag on TOMM2--mCherry, to be used with CoilR labeled with a reporter

Depositor

Kimberly Beatty

Article

Doh et al Proc Natl Acad Sci U S A. 2018 Dec 18;11

Insert

TOMM20-CoilE-mCherry

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

Available since

March 22, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pHH0103_PDCD6IP_BRO1

Plasmid

#109994

Purpose

Protein expression and purification of PDCD6IP_BRO1

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

PDCD6IP_BRO1 (PDCD6IP Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 17, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pHH0103_LRP5_Ldl-recept-b_2

Plasmid

#109807

Purpose

Protein expression and purification of LRP5_Ldl-recept-b_2

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

LRP5_Ldl-recept-b_2

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 17, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pHH0103_UBR5_PABP

Plasmid

#109877

Purpose

Protein expression and purification of UBR5_PABP

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

UBR5_PABP (UBR5 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 17, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pHH0103_NR4A2_Hormone-recep

Plasmid

#109953

Purpose

Protein expression and purification of NR4A2_Hormone-recep

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

NR4A2_Hormone-recep

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 4, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pHH0103_PPARA_Hormone-recep

Plasmid

#109954

Purpose

Protein expression and purification of PPARA_Hormone-recep

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

PPARA_Hormone-recep

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pHH0103_RORA_Hormone-recep

Plasmid

#109957

Purpose

Protein expression and purification of RORA_Hormone-recep

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

RORA_Hormone-recep

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pHH0103_RORC_Hormone-recep

Plasmid

#109959

Purpose

Protein expression and purification of RORC_Hormone-recep

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

RORC_Hormone-recep

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pHH0103_NR1D2_Hormone-recep

Plasmid

#109961

Purpose

Protein expression and purification of NR1D2_Hormone-recep

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

NR1D2_Hormone-recep

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pHH0103_GGA3_Alpha-adaptinC2

Plasmid

#109926

Purpose

Protein expression and purification of GGA3_Alpha-adaptinC2

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

GGA3_Alpha-adaptinC2

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge