We narrowed to 5,627 results for: mcs

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pET21-pelB-LaG-16-4GS-LaG-2

Plasmid

#172764

Purpose

Bacterial expression of dimerized anti-GFP nanobodies LaG-16 and LaG-2 (4GS linker)

Depositor

Michael Rout

Article

Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

Insert

LaG-16-4GS-LaG-2

Tags

6xHIS and pelB leader for periplasmic secretion

Expression

Bacterial

Promoter

T7

Available Since

Aug. 18, 2021

Availability

Academic Institutions and Nonprofits only

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FASTHDR-Cterm-mClover3-Puromycin

Plasmid

#167205

Purpose

Plasmid to clone recombination arms to create homologous recombination donor vector for C-terminal gene tagging with mClover3 and selection with Puromycin

Depositor

Oscar Perez

Article

Perez-Leal et al CRISPR J. 2021 Nov 30. doi: 10.1089/cris

Type

Empty backbone

Use

CRISPR

Tags

mClover3

Expression

Mammalian

Available Since

Dec. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pET21-pelB-LaG-16

Plasmid

#172746

Purpose

Bacterial expression of HIS-tagged anti-GFP nanobody LaG-16; HIS tag preceded by HRV 3C/PreScission cleavage site.

Depositor

Michael Rout

Article

Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

Insert

LaG-16

Tags

6xHIS and pelB leader for periplasmic secretion

Expression

Bacterial

Promoter

T7

Available Since

Aug. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3 HA E2F3B

Plasmid

#236438

Purpose

transient overexpression of E2F3B in mammalian cells

Depositor

Michele Pagano

Article

Clijsters et al Mol Cell. 2019 Jun 20;74(6):1264-1277.e7

Insert

E2F3B (E2F3 Human)

Tags

HA

Expression

Mammalian

Available Since

May 28, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-dCas9-dMQ1-EGFP

Plasmid

#89637

Purpose

Targeted CpG methylation

Depositor

Margaret Goodell

Article

Lei et al Nat Commun. 2017 Jul 11;8:16026. doi: 10

Insert

site-specific DNA-methyltransferase SssI

Use

CRISPR

Tags

3*FLAG, 6*His, and T2A-EGFP

Expression

Mammalian

Mutation

C141S, TGC-->TCC; S317A, AGC-->GCC

Promoter

CMV

Available Since

July 12, 2017

Availability

Academic Institutions and Nonprofits only

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pGFP-Kif5c

Plasmid

#71853

Purpose

mammalian expression of GFP-Kif5c

Depositor

Michelle Peckham

Article

Dunn et al J Cell Sci. 2008 Apr 1;121(Pt 7):1085-95

Insert

Kif5c (Kif5c Rat)

Tags

EGFP

Expression

Mammalian

Mutation

Kif5c starts at AA3

Promoter

CMV

Available Since

Jan. 25, 2016

Availability

Academic Institutions and Nonprofits only

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pFL-SV40-STAT1 5'-UTR

Plasmid

#115353

Purpose

firefly luciferase (Fluc) reporter containing the 5’-UTR of STAT1

Depositor

Ming-Chih Lai

Article

Ku et al Mol Cell Biol. 2018 Dec 11;39(1). pii: M

Insert

STAT1 5'-UTR (STAT1 Human)

Available Since

Sept. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3 HA E2F3A

Plasmid

#236437

Purpose

transient overexpression of E2F3A in mammalian cells

Depositor

Michele Pagano

Article

Clijsters et al Mol Cell. 2019 Jun 20;74(6):1264-1277.e7

Insert

E2F3A (E2F3 Human)

Tags

HA

Expression

Mammalian

Available Since

May 28, 2025

Availability

Academic Institutions and Nonprofits only

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pX552-hsyn DIO GCaMP8f(with gRNA scaffold)

Plasmid

#199580

Purpose

Expresses GCaMP8f in a Cre-dependent manner

Depositor

Bryan Copits

Article

Moffa et al eNeuro. 2024 Jul 5;11(7):ENEURO.0438-23.

Insert

His-DIO GCaMP8f

Use

AAV, CRISPR, and Cre/Lox

Tags

6xHis

Expression

Mammalian

Promoter

hsyn

Available Since

Oct. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti-hU6-enhanced_gRNA-BFP-P2A-PuroR

Plasmid

#230935

Purpose

gRNA cloning vector encoding BFP and puromycin resistance

Depositor

Charles Gersbach

Article

Liu et al Cell Genom. 2025 Feb 4:100766. doi: 10.1

Type

Empty backbone

Use

CRISPR and Lentiviral

Tags

TagBFP

Promoter

hU6

Available Since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

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pET21-pelB-LaG-16-3xFLAG-LaG-2

Plasmid

#172765

Purpose

Bacterial expression of dimerized anti-GFP nanobodies LaG-16 and LaG-2 (3xFLAG linker)

Depositor

Michael Rout

Article

Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

Insert

LaG-16-3xFLAG-LaG-2

Tags

6xHIS and pelB leader for periplasmic secretion

Expression

Bacterial

Promoter

T7

Available Since

Aug. 18, 2021

Availability

Academic Institutions and Nonprofits only

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MIC60-mCherry

Plasmid

#232789

Purpose

Encodes an mCherry-tagged MIC60/IMMT

Depositor

Giulia Bertolin

Article

Bertolin et al Life Sci Alliance. 2021 Apr 5;4(6):e2020

Insert

MICOS complex subunit MIC60 (IMMT Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

March 13, 2025

Availability

Academic Institutions and Nonprofits only

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HSC1-HS4-D4Z4c-GiP

Plasmid

#58541

Purpose

Derived from HSC1-GiP where 2 copies of the HS4 insulator core inside the 3'LTR and the D4Z4-c insulator, are combined upstream of the EF1 alpha promoter

Depositor

James Ellis

Article

Rival-Gervier et al Mol Ther. 2013 Aug;21(8):1536-50. doi: 1

Inserts

Enhanced Green Fluorescence Protein, Puromycin resistance gene
D4Z4 repeat, partial

Use

Retroviral

Expression

Mammalian

Promoter

Human EF1-alpha

Available Since

Sept. 3, 2014

Availability

Academic Institutions and Nonprofits only

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pLV hU6-gRNA hUbC-dSaCas9-KRAB-T2A-Thy1.1

Plasmid

#194278

Purpose

Expresses gRNA and dSaCas9-KRAB from lentiviral vector

Depositor

Charles Gersbach

Article

McCutcheon et al bioRxiv. 2023 May 1:2023.05.01.538906. d

Inserts

humanized dSaCas9 KRAB T2A Thy1.1
gRNA

Use

CRISPR and Lentiviral

Tags

HA

Expression

Mammalian

Promoter

hU6 and hUbC

Available Since

Jan. 13, 2023

Availability

Academic Institutions and Nonprofits only

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dSpyCas9-p300-P2A-CASANOVA

Plasmid

#113129

Purpose

Co-expresses dSpyCas9 fused to the p300 histone acetyltransferase core domain and CASANOVA

Depositor

Dominik Niopek

Article

Bubeck et al Nat Methods. 2018 Oct 30. pii: 10.1038/s

Insert

dCas9-p300-P2A-CASANOVA

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Available Since

Oct. 30, 2018

Availability

Academic Institutions and Nonprofits only

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3543 pMIG Bcl-XL mut8

Plasmid

#8792

Depositor

Stanley Korsmeyer

Article

Cheng et al Mol Cell 2001 Sep;8(3):705-11.

Insert

Bcl-XL (BCL2L1 Human)

Use

Retroviral

Expression

Mammalian

Mutation

G138A, R139L, I140N

Available Since

July 6, 2005

Availability

Academic Institutions and Nonprofits only
peGFP-HDAC1 (pc2447)

Plasmid

#240148

Purpose

Expression vector containing a fusion protein of eGFP and human HDAC1.

Depositor

Cristina Cardoso

Article

Rausch et al Nucleic Acids Res. 2020 Dec 16;48(22):12

Insert

HDAC1 (HDAC1 Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pTrc99S-ssDsbA-CRM197-4xDQNAT

Plasmid

#128403

Purpose

Periplasmic expression of CRM197 with C terminal 4xDQNAT glycosylation sites in E. coli

Depositor

Michael Jewett

Article

Stark et al Sci Adv. 2021 Feb 3;7(6). pii: 7/6/eabe9

Insert

Diphtheria toxin

Tags

4xDQNAT glycosylation tag, 6xHis tag, and DsbA si…

Expression

Bacterial

Mutation

Inactivating G52E mutation

Promoter

trc

Available Since

April 13, 2021

Availability

Academic Institutions and Nonprofits only

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pINDUCER21-puro_TAOK3

Plasmid

#209778

Purpose

doxycycline-inducible expression of TAOK3

Depositor

Michael Wehr

Article

Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

Insert

TAOK3 (TAOK3 Human)

Use

Lentiviral

Tags

3xFLAG

Expression

Mammalian

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pET21-pelB-LaM-6

Plasmid

#172771

Purpose

Bacterial expression of anti-mCherry nanobody LaM-6, with pelB leader and C-term free cysteine and 6xHIS tag.

Depositor

Michael Rout

Article

Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

Insert

LaM-6

Tags

6xHIS and pelB leader for periplasmic secretion

Expression

Bacterial

Promoter

T7

Available Since

Aug. 18, 2021

Availability

Academic Institutions and Nonprofits only

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