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We narrowed to 20,314 results for: puromycin

Showing: 2021 - 2040 of 20314 results

  1. pPyCAG-Nanog(L122A)-IP

    Plasmid
    #62992
    Purpose
    Expresses Mouse Nanog L122A Mutants. It is modified from pPyCAG-Nanog-IP (Addgene Plasmid #13838).
    Depositor
    Shinya Yamanaka
    Insert
    Mouse Nanog L122A Mutant (Nanog Mouse)
    Expression
    Mammalian
    Mutation
    Changed Leucine 122 (123 AA in Mouse Protein) to …
    Available Since
    July 30, 2015
    Availability
    Academic Institutions and Nonprofits only
    62992-plasmid-map-sequence-id-104441

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  2. pSBbi-Puro_LPAR_AKAR-mut

    Plasmid
    #125207
    Purpose
    SB-transposon plasmid for stable expression of unphosphorylatable "dead" version of lipid raft-targeted FRET-based PKA activity reporter
    Depositor
    Eric Davis
    Insert
    LPAR_AKAR-mut
    Use
    Transposon
    Tags
    EV linker, cpVenus[E172] and Lyn tag (GCIKSKRKD),…
    Expression
    Mammalian
    Mutation
    FoxO1 Thr-24 changed to Val rendering this inacti…
    Promoter
    EF1a/RPBSA
    Available Since
    May 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    125207-plasmid-map-sequence-id-243606

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  3. pResQ shFEN3 3XF-FEN1 D181A

    Plasmid
    #17753
    Depositor
    Sheila Stewart
    Insert
    Flap Endonuclease I (FEN1 Human)
    Use
    Lentiviral and RNAi
    Tags
    Triple Flag
    Expression
    Mammalian
    Mutation
    D181A
    Available Since
    April 29, 2008
    Availability
    Academic Institutions and Nonprofits only

  4. pKG212

    Plasmid
    #69758
    Purpose
    Retroviral expression of CENP-T aa 1-242
    Depositor
    Iain Cheeseman
    Insert
    CENP-T aa 1-242 (CENPT Human)
    Use
    Retroviral
    Tags
    LacI-NLS and mCherry
    Expression
    Mammalian
    Mutation
    e.coli optimized sequence
    Available Since
    Nov. 18, 2015
    Availability
    Academic Institutions and Nonprofits only

  5. pIRESpuro3+mRFP-Utr_CH

    Plasmid
    #64358
    Purpose
    bicistronic expression of Utrophin-mRFP and Puro resistance
    Depositor
    Matthieu Piel
    Insert
    Utrophin-mRFP (UTRN Human)
    Tags
    IRES-Puro and mRFP
    Expression
    Mammalian
    Mutation
    Q236R; aa1-261 Utrophin present
    Promoter
    CMV
    Available Since
    May 18, 2015
    Availability
    Academic Institutions and Nonprofits only
    64358-plasmid-map-sequence-id-196884

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  6. p663-UBC-V5-dsRed_IDG-K

    Plasmid
    #135262
    Purpose
    Gateway destination clone of dsRed (as control) tagged with N-terminal V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics
    Depositor
    Ben Major
    Insert
    V5-dsRed
    Use
    Lentiviral; Gateway destination
    Tags
    V5
    Expression
    Mammalian
    Promoter
    Ubiquitin
    Available Since
    Jan. 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    135262-plasmid-map-sequence-id-268083

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  8. pPyCAG-Nanog(Y136A)-IP

    Plasmid
    #62995
    Purpose
    Expresses Mouse Nanog Y136A Mutants. It is modified from pPyCAG-Nanog-IP (Addgene Plasmid #13838).
    Depositor
    Shinya Yamanaka
    Insert
    Mouse Nanog Y136A Mutant (Nanog Mouse)
    Expression
    Mammalian
    Mutation
    Changed Tyrosine 136 (137 AA in Mouse Protein) to…
    Available Since
    July 30, 2015
    Availability
    Academic Institutions and Nonprofits only
    62995-plasmid-map-sequence-id-104597

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  9. HA-mRIPK3 T231A/S232A-Flag/cHA-pTRIPZ vector

    Plasmid
    #73707
    Purpose
    lentiviral expression of mRIPK3 mutant
    Depositor
    Francis Chan
    Insert
    RIPK3 (Ripk3 Mouse)
    Use
    Lentiviral
    Tags
    Flag and HA
    Expression
    Mammalian
    Mutation
    T231A/S232A
    Promoter
    CMV/tet
    Available Since
    March 28, 2016
    Availability
    Academic Institutions and Nonprofits only

  10. HA-mRIPK3 AAAA-Flag/cHA-pTRIPZ vector

    Plasmid
    #73702
    Purpose
    lentiviral expression of mRIPK3 mutant
    Depositor
    Francis Chan
    Insert
    RIPK3 (Ripk3 Mouse)
    Use
    Lentiviral
    Tags
    Flag and HA
    Expression
    Mammalian
    Mutation
    448-AAAA-451
    Promoter
    CMV/tet
    Available Since
    March 29, 2016
    Availability
    Academic Institutions and Nonprofits only

  11. pPyCAG-Nanog(K137A)-IP

    Plasmid
    #62996
    Purpose
    Expresses Mouse Nanog K137A Mutants. It is modified from pPyCAG-Nanog-IP (Addgene Plasmid #13838).
    Depositor
    Shinya Yamanaka
    Insert
    Mouse Nanog K137A Mutant (Nanog Mouse)
    Expression
    Mammalian
    Mutation
    Changed Lysine 137 (138 AA in Mouse Protein) to A…
    Available Since
    July 30, 2015
    Availability
    Academic Institutions and Nonprofits only
    62996-plasmid-map-sequence-id-104598

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  12. pPyCAG-Nanog(Y119A)-IP

    Plasmid
    #62991
    Purpose
    Expresses Mouse Nanog Y119A Mutants. It is modified from pPyCAG-Nanog-IP (Addgene Plasmid #13838).
    Depositor
    Shinya Yamanaka
    Insert
    Mouse Nanog Y119A Mutant (Nanog Mouse)
    Expression
    Mammalian
    Mutation
    Changed Tyrosine 119 (120 AA in Mouse Protein) to…
    Available Since
    July 30, 2015
    Availability
    Academic Institutions and Nonprofits only
    62991-plasmid-map-sequence-id-104440

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  13. pYJ41-AAVS1-XLone-Mafa

    Plasmid
    #131084
    Purpose
    Donor plasmid of targeted inducible-Mafa knockin
    Depositor
    Xiaojun Lian
    Insert
    MAFA (MAFA Human)
    Use
    CRISPR
    Expression
    Mammalian
    Available Since
    Oct. 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    131084-plasmid-map-sequence-id-256890

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  14. pMbd3-GGSG-AID-3xF_PP

    Plasmid
    #235507
    Purpose
    Targeting construct to make C-term fusion with GGSG-AID-3xFLAG, roxed Pgk-Puro
    Depositor
    Brian Hendrich
    Insert
    Mbd3 (Mbd3 Mouse)
    Use
    Mouse Targeting
    Tags
    AID, 3xFLAG
    Mutation
    None
    Promoter
    PGK
    Available Since
    May 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    235507-plasmid-map-sequence-id-474013

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  16. pLVX ALIX-GFP S718/721A

    Plasmid
    #138583
    Purpose
    Expresses fluorescent ALIX S718/721A in mammalian cells
    Depositor
    Wesley Sundquist
    Insert
    ALIX (PDCD6IP Human)
    Use
    Lentiviral
    Tags
    GFP and h30
    Expression
    Mammalian
    Mutation
    changed serines 718 and 721 to alanines
    Promoter
    TREtight
    Available Since
    Dec. 10, 2024
    Availability
    Academic Institutions and Nonprofits only
    138583-plasmid-map-sequence-id-322303

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  17. pCW57.1-GFP_mmu-miR-292b-3p_sponge

    Plasmid
    #132622
    Purpose
    Expresses a TurboGFP transcript with test microRNA sponge for mmu-miR-292b-3p as 3'UTR; serves as template for other microRNA sponges
    Depositor
    Constance Ciaudo
    Insert
    stop codon, mmu-miR-292b-3p sponge sequence and polyA signal
    Use
    Lentiviral; Doxycycline inducible
    Expression
    Mammalian
    Promoter
    TRE
    Available Since
    Aug. 15, 2022
    Availability
    Academic Institutions and Nonprofits only
    132622-plasmid-map-sequence-id-259572

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  18. pX459-puro-hCASP8

    Plasmid
    #185378
    Purpose
    For mammalian expression of guide RNA: AAGCAATCTGTCCTTCCTGA that targets human CASP8 (Caspase 8)
    Depositor
    Ayaz Najafov
    Insert
    CASP8 (CASP8 Human)
    Expression
    Mammalian
    Mutation
    WT
    Available Since
    June 8, 2022
    Availability
    Academic Institutions and Nonprofits only
    185378-plasmid-map-sequence-id-363829

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  19. pX459-puro-hARAF-1

    Plasmid
    #185367
    Purpose
    For mammalian expression of guide RNA: caccgTGGTCTACCGACTCATCAAG that targets human ARAF
    Depositor
    Ayaz Najafov
    Insert
    ARAF (ARAF Human)
    Expression
    Mammalian
    Mutation
    WT
    Available Since
    June 7, 2022
    Availability
    Academic Institutions and Nonprofits only
    185367-plasmid-map-sequence-id-363617

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  20. pLKO-puro-mAK1-shRNA-1

    Plasmid
    #185364
    Purpose
    For mammalian expression of shRNA: ATCTTGACTCCCTGAAGTAAC that targets mouse AK1 (adenylate kinase)
    Depositor
    Ayaz Najafov
    Insert
    AK1 (Ak1 Mouse)
    Expression
    Mammalian
    Mutation
    WT
    Available Since
    June 7, 2022
    Availability
    Academic Institutions and Nonprofits only
    185364-plasmid-map-sequence-id-363607

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Showing: 2021 - 2040 of 20314 results