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pBLADE(FP6**)-mCherry

Plasmid

#168049

Purpose

Expresses the synthetic gene BLADE FP6 under the constitutive J23101** promoter. mCherry expression is controlled by the PBAD promoter.

Depositor

Barbara Di Ventura

Publication

Romano et al Nat Chem Biol. 2021 Apr 26. pii: 10.1038

Inserts

Blue light-inducible AraC dimers in Escherichia coli
mCherry

Expression

Bacterial

Promoter

J23101** and PBAD

Available since

May 11, 2021

Availability

Academic Institutions and Nonprofits only

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hu6 HGPS sgRNA expression and ABE7.10max VRQR lentiviral plasmid

Plasmid

#154429

Purpose

hu6 HGPS sgRNA expression and ABE7.10max VRQR lentiviral plasmid

Depositor

David Liu

Publication

Koblan et al Nature. 2021 Jan;589(7843):608-614. doi:

Insert

hu6 HGPS sgRNA expression and ABE7.10max VRQR lentiviral plasmid

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

VRQR point mutations in SpCas9

Available since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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Nuclear cpGFP

Plasmid

#215707

Purpose

Expresses cpGFP in the nucleus

Depositor

Katharine Diehl

Publication

Smith et al Cell Chem Biol. 2025 Jan 21:S2451-9456(2

Insert

cpGFP

Use

Lentiviral

Tags

FLAG, HA, and NLS

Expression

Mammalian

Mutation

circularly permuted GFP

Promoter

CMV

Available since

March 12, 2024

Availability

Academic Institutions and Nonprofits only

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pAtetO_6xHis-Klentaq

Plasmid

#157862

Purpose

Plasmid for the aTc-inducible expression of the Klentaq

Depositor

Andrew Ellington

Publication

Milligan et al Biochemistry. 2018 Aug 7;57(31):4607-461

Insert

Klentaq

Tags

6xHis

Expression

Bacterial

Promoter

tet PA

Available since

Aug. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pT7-7 asyn E46K

Plasmid

#105728

Purpose

Bacterial expression of mutant human alpha synuclein

Depositor

Hilal Lashuel

Publication

Mbefo et al J Biol Chem. 2015 Apr 10;290(15):9412-27

Insert

alpha-synuclein E46K (SNCA Human)

Expression

Bacterial

Mutation

E46K

Promoter

T7

Available since

April 2, 2018

Availability

Academic Institutions and Nonprofits only

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pBS-ermGFP

Plasmid

#27172

Depositor

Michela Lizier

Publication

Lizier et al FEMS Microbiol Lett. 2010 Jul 1. 308(1):

Insert

erythromycin ribosomal methylase promoter region fused with modified green fluorescent protein 5

Expression

Bacterial

Mutation

N/A

Available since

Jan. 24, 2011

Availability

Academic Institutions and Nonprofits only

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PhiC31-Neo-ins-5xTetO-pEF-H2B-Citrine-ins

Plasmid

#78099

Purpose

Nuclear fluorescent reporter in mammalian cells, phiC31 attB for integration, can be silenced with TetR-CR or rTetR-CR (e.g. CR can be KRAB or DNMT3B).

Depositor

Michael Elowitz

Publication

Bintu et al Science. 2016 Feb 12;351(6274):720-4. do

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Histone H2B - Citrine (H2BC21 Synthetic, Human)

Use

Synthetic Biology

Expression

Mammalian

Promoter

5xTetO pEF alpha

Available since

June 15, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles
pTOX3

Plasmid

#127448

Purpose

Encodes Tse2 toxin. CAM-R

Depositor

Matthew Waldor

Publication

Lazarus et al Appl Environ Microbiol. 2019 Jun 14. pii

Insert

tse2 toxin

Expression

Bacterial

Promoter

pRha

Available since

July 12, 2019

Availability

Academic Institutions and Nonprofits only

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Mitochondrial cpGFP

Plasmid

#215711

Purpose

Expresses cpGFP in the mitochondria

Depositor

Katharine Diehl

Publication

Smith et al Cell Chem Biol. 2025 Jan 21:S2451-9456(2

Insert

cpGFP

Use

Lentiviral

Tags

FLAG, HA, and mitochondrial localization sequence…

Expression

Mammalian

Mutation

circularly permuted GFP

Promoter

CMV

Available since

March 12, 2024

Availability

Academic Institutions and Nonprofits only

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ATG9A-mCherry

Plasmid

#197393

Purpose

Expresses ATG9A in mammalian cells

Depositor

Juan Bonifacino

Publication

De Pace et al PLoS Genet. 2018 Apr 26;14(4):e1007363.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

ATG9A (ATG9A Human)

Tags

mCh

Expression

Mammalian

Available since

March 28, 2023

Availability

Academic Institutions and Nonprofits only

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pCAGGs-BFP

Plasmid

#127348

Purpose

Expresses BFP constitutively via the CAGGS promoter in mammalian cells

Depositor

Phil Sharp

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Blue Fluorescent Protein

Expression

Mammalian

Promoter

CAGGS Promoter

Available since

March 4, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pEB1-SCFP3A

Plasmid

#103970

Purpose

Plasmid encoding SCFP3A

Depositor

Philippe Cluzel

Publication

Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

Insert

SCFP3A

Use

Low copy

Expression

Bacterial

Mutation

Mutations relative to wild-type GFP (F64L, S65T, …

Promoter

proC

Available since

Dec. 14, 2017

Availability

Academic Institutions and Nonprofits only

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pQE80L-SpyCatcher-ELP-GFP

Plasmid

#69835

Purpose

Bacterial expression plasmid containing SpyCatcher-ELP-GFP fusion. SpyCatcher forms a covalent bond with SpyTag and can be used to label plasma membrane localized SpyTag-C1C2 in live cells.

Depositor

Viviana Gradinaru

Publication

Bedbrook et al Chem Biol. 2015 Aug 20;22(8):1108-21. do

Insert

SpyCatcher-ELP-GFP

Tags

6x His Tag, GFP, and TEV Tag

Expression

Bacterial

Promoter

T5 promoter/lac operator element

Available since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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mEmerald-Beta-Catenin-20

Plasmid

#54017

Purpose

Localization: Tight Junctions, Excitation: 487, Emission: 509

Depositor

Michael Davidson

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Beta-Catenin (Ctnnb1 Mouse)

Tags

mEmerald

Expression

Mammalian

Promoter

CMV

Available since

June 16, 2014

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

3 Articles
pET11a_His-TEV-PLpro (nsp3) (SARS-CoV-2)

Plasmid

#169192

Purpose

To express SARS-CoV-2 Papain-like protease in E. coli

Depositor

John Diffley

Publication

Lim et al Biochem J. 2021 Jul 16;478(13):2517-2531

Insert

6His-TEV-nsp3_E746-K1060 (pp1ab_E1564-K1878) (ORF1ab Synthetic, SARS-CoV-2)

Use

Unspecified

Tags

6His-TEV

Mutation

Codon optimised for E. coli

Promoter

T7

Available since

July 6, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV-PARP1-3xFlag-7xKQ

Plasmid

#111576

Purpose

Expresses full length PARP1 with K to Q mutations in automodification domain in mammalian cells

Depositor

Thomas Muir

Publication

Liszczak et al Nat Chem Biol. 2018 Jul 16. pii: 10.1038

Insert

PARP1 (PARP1 Human)

Tags

3xFlag

Expression

Mammalian

Mutation

lysine to glutamine mutation at the following ami…

Promoter

CMV

Available since

July 18, 2018

Availability

Academic Institutions and Nonprofits only

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TRE:GFP

Plasmid

#163036

Purpose

FLiCRE reporter gene for neuronal expression

Depositor

Alice Ting

Publication

Kim et al Cell. 2020 Dec 23;183(7):2003-2019.e16.

Insert

GFP

Use

AAV

Expression

Mammalian

Promoter

TRE

Available since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pLBH532_MBP-Cas14b1 expression

Plasmid

#112505

Purpose

Expresses 10xHis-MBP fused to Cas14b1 under t7 promoter

Depositor

Jennifer Doudna

Publication

Harrington et al Science. 2018 Oct 18. pii: science.aav42

Insert

Cas14b1

Use

CRISPR

Tags

MBP

Expression

Bacterial

Promoter

T7

Available since

Oct. 26, 2018

Availability

Academic Institutions and Nonprofits only

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pRB1080

Plasmid

#71309

Purpose

Expresses VQR Cas9 variant with eef-1A.1 (eft-3) promoter

Depositor

Andrew Fire

Publication

Bell et al Genetics. 2015 Dec 17. pii: genetics.115

Insert

VQR Cas9 variant

Use

CRISPR

Expression

Worm

Promoter

eef-1A.1 (eft-3)

Available since

Dec. 17, 2015

Availability

Academic Institutions and Nonprofits only

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TDP-REGv2(mScarlet-FLAG)#7 in pTwist-CMV backbone

Plasmid

#216152

Purpose

Expresses mScarlet in cells with TDP-43 loss-of-function. This plasmid has one of the best dynamic ranges of those tested (~100-fold) in SK-N-BE2 cells. It uses a cryptic exon. [Code 'A11'] Note: It is not recommended to produce lentiviruses containing TDP-REG sequences – see Depositor Comments

Depositor

Pietro Fratta

Publication

Wilkins et al Science. 2024 Oct 4;386(6717):61-69. doi

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

mScarlet with cryptic exon and C-terminal FLAG

Expression

Mammalian

Promoter

CMV

Available since

July 1, 2024

Availability

Academic Institutions and Nonprofits only

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