We narrowed to 4,217 results for: Alk

1. LCV2_LacZ_sgRNA_2

   Plasmid

   #155093

   Purpose

   lentiviral plasmid expressing Cas9 and gRNA targeting LacZ

   Depositor

   Jason Moffat

   Article

   Aregger et al Nat Metab. 2020 Jun;2(6):499-513. doi: 1

   Insert

   LacZ_sgRNA_2
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 promoter

   Available since

   Aug. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pPB-TRE-V5-TurboID-linker-T2A-GR-bGH polyA > mPGK-BlastR-SV40 polyA

   Plasmid

   #218910

   Purpose

   PiggyBac transposition

   Depositor

   Sven Eyckerman

   Article

   Delhaye et al Cell Rep Methods. 2024 Jul 15;4(7):10081

   Insert

   V5-TurboID-T2A-huGR/BlastR (NR3C1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   2363G>C and 4155C>T and 4729T>G and 4731…

   Promoter

   Available since

   June 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBactin-mOrange2-NEDD1-gTBD

   Plasmid

   #196858

   Purpose

   Expression of the gamma-tubulin-binding domain (gTBD) of NEDD1 fused to mOrange. Used to displace endogenous gamma-TuRC from the centrosome.

   Depositor

   Frank Bradke

   Article

   Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

   Insert

   mOrange2-N-gTBD (NEDD1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Chicken bactin (plus Chicken bactin intron)

   Available since

   May 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLRC1-NEK10p:NEK10-3XFLAG

   Plasmid

   #137030

   Purpose

   Lentiviral vector for expressing NEK10 under the control of the endogenous NEK10 promoter

   Depositor

   David Sabatini

   Article

   Chivukula et al Nat Med. 2020 Jan 20. pii: 10.1038/s4159

   Insert

   NIMA-like kinase 10 (NEK10 Human)
   

   Use

   Lentiviral

   Tags

   3XFLAG

   Expression

   Mutation

   Promoter

   human NEK10 endogenous promoter

   Available since

   Feb. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRetroX-mCRT-GPI-IRES-DsRed

   Plasmid

   #223711

   Purpose

   Mammalian expression of mouse CRT-GPI

   Depositor

   Judy Lieberman

   Article

   Sen Santara et al Nature. 2023 Apr;616(7956):348-356. doi:

   Insert

   Calreticulin-GPI (Calr Mouse)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCMV-CRT-Fc FL

   Plasmid

   #221853

   Purpose

   Express human Calreticulin (full-length) with C-terminal human IgG1Fc tag in mammalian cell.

   Depositor

   Judy Lieberman

   Article

   Sen Santara et al Nature. 2023 Apr;616(7956):348-356. doi:

   Insert

   calreticulin (CALR Human)
   

   Use

   Tags

   Human IgG1Fc tag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBactin-EGFP-NEDD1-gTBD

   Plasmid

   #196857

   Purpose

   Expression of the gamma-tubulin-binding domain (gTBD) of NEDD1 fused to enhanced (E) GFP. Used to displace endogenous gamma-TuRC from the centrosome.

   Depositor

   Frank Bradke

   Article

   Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

   Insert

   EGFP-N-gTBD (NEDD1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Chicken bactin (plus Chicken bactin intron)

   Available since

   May 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pUB6-HuMGF

   Plasmid

   #190782

   Purpose

   Expresses membrane-bound form of human SCF in mammalian cells

   Depositor

   Jakob Reiser

   Article

   Zhang et al Retrovirology. 2010 Jan 25;7:3. doi: 10.

   Insert

   human stem cell factor (KITLG Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   UbC

   Available since

   Nov. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. EphA2 430-976 pcDNA3.1

   Plasmid

   #102738

   Purpose

   Expression of c terminal EphA2 in mammalian cells

   Depositor

   Jin Chen

   Article

   Amato et al Cancer Res. 2016 Jan 15;76(2):305-18. do

   Insert

   EphA2 N terminal (430-976aa) (Epha2 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. BE3-NLS-NLS-P2A-BLAST

    Plasmid

    #135348

    Purpose

    Expression of BE3 (Base editor generation three) with dual C-terminal NLS in a single operon encoding P2A blasticidine resistance

    Depositor

    Alex Hewitt

    Article

    Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

    Insert

    BE3-2XNLS
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    Available since

    Oct. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET51b-Strep-hAGT-His

    Plasmid

    #167276

    Purpose

    Overexpression of the human o_-alkylguanine-dna alkyltransferase in E. coli

    Depositor

    Kai Johnsson

    Article

    Wilhelm et al Biochemistry. 2021 Aug 24;60(33):2560-25

    Insert

    hAGT (AGT Human)
    

    Use

    Tags

    His x10 and Strep-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. LCV2_mClover3_LacZ

    Plasmid

    #155103

    Purpose

    lentiviral plasmid expressing mClover3, Cas9 and a gRNA targeting LacZ

    Depositor

    Jason Moffat

    Article

    Aregger et al Nat Metab. 2020 Jun;2(6):499-513. doi: 1

    Insert

    LacZ_sgRNA_2
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 promoter

    Available since

    Aug. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. LCV2_BFP2

    Plasmid

    #155097

    Purpose

    Lentiviral backbone for cloning and expression of U6 driven gRNAs with Esp3I/BsmBI cloning sites, puromycin selection and BFP2 expression

    Depositor

    Jason Moffat

    Article

    Aregger et al Nat Metab. 2020 Jun;2(6):499-513. doi: 1

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pLNHA-C1-HsGIGYF2_AE

    Plasmid

    #148550

    Purpose

    Mammalian Expression of HsGIGYF2

    Depositor

    Elisa Izaurralde

    Article

    Peter et al Genes Dev. 2017 Jun 1;31(11):1147-1161.

    Insert

    HsGIGYF2 (GIGYF2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    one silent mutation and one deletion of Q1211 com…

    Promoter

    Available since

    Nov. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pSLIKneo 3XFLAG-wtGATA6-3XAU1_RNAi resistant

    Plasmid

    #72616

    Purpose

    Expresses an inducible and RNAi resistant, N-terminally 3X-FLAG tagged, C-terminally 3X-AU1 tagged wtGATA6

    Depositor

    Kevin Janes

    Article

    Chitforoushzadeh et al Sci Signal. 2016 Jun 7;9(431):ra59. doi:

    Insert

    GATA Binding Protein 6 (GATA6 Human)
    

    Use

    Lentiviral

    Tags

    3XAU1 and 3XFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    TRE tight

    Available since

    July 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pT7-EGFP-C1-HsGIGYF2_AE

    Plasmid

    #148577

    Purpose

    Mammalian Expression of HsGIGYF2

    Depositor

    Elisa Izaurralde

    Article

    Peter et al Genes Dev. 2017 Jun 1;31(11):1147-1161.

    Insert

    HsGIGYF2 (GIGYF2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    one silent mutation and one deletion of Q1211 com…

    Promoter

    Available since

    Nov. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. LCV2_mCherry_LacZ_sgRNA

    Plasmid

    #155108

    Purpose

    lentiviral plasmid expressing mCherry, Cas9 and a gRNA targeting LacZ

    Depositor

    Jason Moffat

    Article

    Aregger et al Nat Metab. 2020 Jun;2(6):499-513. doi: 1

    Insert

    LacZ_sgRNA_2
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 promoter

    Available since

    Aug. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pEN_TT 3XFLAG-wtGATA6-3XAU1_RNAi resistant

    Plasmid

    #72922

    Purpose

    Gateway entry vector for an inducible N-terminally 3XFLAG-tagged and C-terminally AU1-tagged, RNAi resistant human wtGATA6

    Depositor

    Kevin Janes

    Article

    Chitforoushzadeh et al Sci Signal. 2016 Jun 7;9(431):ra59. doi:

    Insert

    GATA Binding Protein 6 (GATA6 Human)
    

    Use

    Entry vector for gateway cloning

    Tags

    3XAU1 and 3XFLAG

    Expression

    Bacterial

    Mutation

    Promoter

    TRE tight

    Available since

    July 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. EphA2 1-427 pcDNA3.1

    Plasmid

    #102737

    Purpose

    Expression of N terminal EphA2 in mammalian cells

    Depositor

    Jin Chen

    Article

    Amato et al Cancer Res. 2016 Jan 15;76(2):305-18. do

    Insert

    EphA2 N terminal (1-427aa) (Epha2 Mouse)
    

    Use

    Tags

    6xHis and myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pScalps-Puro-FLAG_HA-Regnase-1 D141N

    Plasmid

    #222663

    Purpose

    Lentiviral vector to express mouse Regnase-1 D141N mutant tagged with FLAG-HA at N-term

    Depositor

    Silvia Monticelli

    Article

    Bataclan et al Life Sci Alliance. 2024 Jun 3;7(8):e2024

    Insert

    Zc3h12a (Zc3h12a Mouse)
    

    Use

    Lentiviral

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    mutated aspartic acid 141 to asparagine to abroga…

    Promoter

    SFFV

    Available since

    Jan. 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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