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pCSC-GFP-NES-EGFP

Plasmid

#221810

Purpose

Lentiviral vector expressing green fluorescent protein (GFP) fused with nuclear export sequence (NES).

Depositor

Baojin Ding

Article

Akter et al J Neurosci. 2024 Apr 10;44(15):e17282320

Insert

GFP-NES-EGFP

Use

Lentiviral

Tags

NES (internal tag)

Expression

Mammalian

Promoter

CMV

Available Since

July 2, 2024

Availability

Academic Institutions and Nonprofits only

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pMuLE_ENTR_12GLI-FLuc_R4-R3

Plasmid

#113712

Purpose

Single pathway reporter; hedgehog promoter-driven expression of firefly luciferase; MultiSite Gateway entry vector.

Depositor

Manfred Ogris

Article

Maier et al PLoS One. 2019 Dec 20;14(12):e0226570. d

Insert

12GLI-FLuc

Use

Gateway Cloning

Expression

Mammalian

Available Since

Jan. 22, 2020

Availability

Academic Institutions and Nonprofits only

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P307-FBP_6

Plasmid

#139697

Purpose

T-DNA encoding Fungal Bioluminescent Pathway Components, control pathway

Depositor

Daniel Voytas

Article

Khakhar et al Elife. 2020 Mar 25;9. pii: 52786. doi: 1

Insert

pLHB1B1:NnCPH:tSlH4, p35S_Short_TMV 5':AsNpga:tAtug7, pAtUbi1:NnH3H:tAtuNos, pCmYLCV:NnHisps:tHsp, p35S:NnLuz:tAtuOcs

Use

Luciferase

Expression

Plant

Promoter

various

Available Since

May 29, 2020

Availability

Academic Institutions and Nonprofits only

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Plasmid L5 attB::Pleft* mScarlet

Plasmid

#169410

Purpose

Chromosomally-Integrated Fluorescent Mycobacterium tuberculosis Reporter Constructs for mScarlet

Depositor

Clifton Barry III

Article

Kolbe et al Front Microbiol. 2020 Dec 9;11:591866. d

Inserts

pLeft
mScarlet proper

Use

Unspecified

Available Since

May 12, 2021

Availability

Academic Institutions and Nonprofits only

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Plasmid L5 attB::Pleft* mWasabi

Plasmid

#169409

Purpose

Chromosomally-Integrated Fluorescent Mycobacterium tuberculosis Reporter Constructs for mWasabi

Depositor

Clifton Barry III

Article

Kolbe et al Front Microbiol. 2020 Dec 9;11:591866. d

Inserts

pLeft
mWasabi

Use

Unspecified

Available Since

May 12, 2021

Availability

Academic Institutions and Nonprofits only

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single cut NH-donor

Plasmid

#83575

Purpose

eGFP reporter donor containing single SA cutting site for NHEJ knock-in

Depositor

Bo Feng

Article

He et al Nucleic Acids Res. 2016 May 19;44(9):e85

Insert

eGFP

Use

Nhej donor

Available Since

Nov. 11, 2016

Availability

Academic Institutions and Nonprofits only

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pMuLE_ENTR_PTCH1-mTurquoise2_R4-R3

Plasmid

#113864

Purpose

Single pathway reporter; hedgehog promoter-driven expression of mTurquoise2; MultiSite Gateway entry vector.

Depositor

Manfred Ogris

Article

Maier et al PLoS One. 2019 Dec 20;14(12):e0226570. d

Inserts

PTCH1 (hedgehog dependent promoter)
mTurquoise2

Use

Gateway Cloning

Expression

Mammalian

Available Since

Jan. 22, 2020

Availability

Academic Institutions and Nonprofits only

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pHR iRFP-ZtSH2

Plasmid

#188627

Purpose

Fluorescent reporter for CD3ε/ZtSH2 pYtag biosensor

Depositor

Jared Toettcher

Article

Farahani et al Elife. 2023 May 22;12:e82863. doi: 10.75

Insert

ZtSH2

Use

Lentiviral

Tags

iRFP

Expression

Mammalian

Promoter

SFFV

Available Since

Aug. 16, 2022

Availability

Academic Institutions and Nonprofits only

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Plasmid L5 attB:Psmyc triple red

Plasmid

#169413

Purpose

Chromosomally-Integrated Fluorescent Mycobacterium tuberculosis Reporter Constructs for triple red

Depositor

Clifton Barry III

Article

Kolbe et al Front Microbiol. 2020 Dec 9;11:591866. d

Inserts

mScarlet proper
COmScarlet
mRuby3

Use

Unspecified

Available Since

May 12, 2021

Availability

Academic Institutions and Nonprofits only

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CMV-OMM-jRCaMP1b

Plasmid

#127871

Purpose

Red-shifted fluorescent reporter for mitochondrial outer membrane Ca2+

Depositor

Timothy Ryan

Article

Ashrafi et al Neuron. 2019 Dec 10. pii: S0896-6273(19)

Insert

OMM-jRCaMP1b (Red-shifted outer membrane Ca2+ indicator)

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pCAFNF-GFP

Plasmid

#13772

Purpose

Flp recombinase-dependent expression of GFP, i.e. GFP is only expressed in the presence of Flp due to a transcriptional stop in between the FRT sites.

Depositor

Connie Cepko

Article

Matsuda et al Proc Natl Acad Sci U S A. 2007 Jan 16;10

Insert

EGFP

Use

Flp/frt

Expression

Mammalian

Promoter

CAG

Available Since

April 20, 2007

Availability

Academic Institutions and Nonprofits only

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pGilesSHdI_redgreen_2395A_His6_triple red_pLeft1806_His6_double green

Plasmid

#170048

Purpose

Tri-Responsive Reporter Gene Construct for redgreen, triple red, and double green

Depositor

Clifton Barry III

Article

Kolbe et al Front Microbiol. 2020 Dec 9;11:591866. d

Inserts

mScarlet
COmScarlet
mRuby3
mWasabi dN
mWasabi CO

Use

Unspecified

Available Since

May 12, 2021

Availability

Academic Institutions and Nonprofits only

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pWaldo-GFPe-SH1446

Plasmid

#112507

Purpose

Expresses SH1446 in E. coli with a C-terminal TEV-GFP-His(8) tag.

Depositor

Simon Newstead

Article

Minhas et al Elife. 2018 Jul 3;7. pii: 34995. doi: 10

Insert

POT family transporter

Tags

C terminal TEV GFP octa-His affinity tag

Expression

Bacterial

Promoter

T7

Available Since

Nov. 29, 2018

Availability

Academic Institutions and Nonprofits only

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double cut NH-donor

Plasmid

#83576

Purpose

eGFP reporter donor containing double SA cutting sites for NHEJ knock-in

Depositor

Bo Feng

Article

He et al Nucleic Acids Res. 2016 May 19;44(9):e85

Insert

eGFP

Use

Nhej donor

Available Since

Nov. 11, 2016

Availability

Academic Institutions and Nonprofits only

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pY2

Plasmid

#218115

Purpose

Activity reporter backbone with an empty protease cassette (BsaI) under (lexA-box)3PminCYC1 promoter and an empty substrate cassette (BsmBI) under pGAL1 promoter.

Depositor

Carl Denard

Article

Martinusen et al Protein Eng Des Sel. 2024 Jan 29;37:gzae

Type

Empty backbone

Expression

Bacterial and Yeast

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pNMT

Plasmid

#42578

Depositor

James Hurley

Article

Carlson et al Proc Natl Acad Sci U S A. 2012 Oct 16;10

Insert

N-myristoyl tranferase (NMT1 Budding Yeast)

Expression

Bacterial

Mutation

two silent mutations T690C and T942C

Promoter

T7

Available Since

Aug. 12, 2013

Availability

Academic Institutions and Nonprofits only
BFP Homology Donor

Plasmid

#149372

Purpose

Promoter-less BFP cDNA with two homology arms to the lenti-CDDR reporter for HDR detection

Depositor

Tarek Abbas

Article

Eki et al Nucleic Acids Res. 2020 Oct 17. pii: 592

Insert

CDDR BFP Homology Donor

Expression

Mammalian

Available Since

Oct. 7, 2020

Availability

Academic Institutions and Nonprofits only

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8xGliBS-IVS2-mCherry-NLS-Odc1-polyA-Tol2

Plasmid

#84603

Purpose

Tol2-based hedgehog signal reporter plasmid for zebrafish transgenesis

Depositor

James Chen

Article

Mich et al PLoS One. 2014 Jul 28;9(7):e103661. doi:

Insert

mCherry

Use

Zebrafish transgenesis

Tags

ODC1 destabilizing element

Promoter

8xGliBS-delta-crystallin minimal promoter

Available Since

Oct. 24, 2016

Availability

Academic Institutions and Nonprofits only

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Plasmid L5 attB:Psmyc double blue

Plasmid

#170050

Purpose

Chromosomally-Integrated Fluorescent Mycobacterium tuberculosis Reporter Constructs for double blue

Depositor

Clifton Barry III

Article

Kolbe et al Front Microbiol. 2020 Dec 9;11:591866. d

Inserts

mTagBFP2
CO BFP

Use

Unspecified

Available Since

May 19, 2021

Availability

Academic Institutions and Nonprofits only

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pAP24

Plasmid

#70190

Purpose

Modular vector for secreted luciferase reporter expression

Depositor

Wiep Klaas Smits

Article

Oliveira Paiva et al ACS Synth Biol. 2016 Jun 23.

Insert

sLucopt

Use

Synthetic Biology

Expression

Bacterial

Promoter

Ptet

Available Since

June 27, 2016

Availability

Academic Institutions and Nonprofits only

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