We narrowed to 6,025 results for: plasmid dna

1. pL6_3xG34-DCAF8L2_X1

   Plasmid

   #191518

   Purpose

   Expression of nuclear v8 ZF-DdCBE in mammalian cells (C-terminal DddAN, Right)

   Depositor

   David Liu

   Article

   Willis et al Nat Commun. 2022 Nov 23;13(1):7204. doi:

   Insert

   ZF-DdCBE 3xG34-DCAF8L2 X1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pL7_3xG35-EMILIN2_X1

   Plasmid

   #191517

   Purpose

   Expression of nuclear v8 ZF-DdCBE in mammalian cells (C-terminal DddAC, Left)

   Depositor

   David Liu

   Article

   Willis et al Nat Commun. 2022 Nov 23;13(1):7204. doi:

   Insert

   ZF-DdCBE 3xG35-EMILIN2 X1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pB6_R13-ND51_v8_X1

   Plasmid

   #191512

   Purpose

   Expression of mitochondrial v8 ZF-DdCBE in mammalian cells (C-terminal DddAN, Right)

   Depositor

   David Liu

   Article

   Willis et al Nat Commun. 2022 Nov 23;13(1):7204. doi:

   Insert

   v8 ZF-DdCBE R13-ND51 X1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pDGO186N-KS3

   Plasmid

   #174303

   Purpose

   Replicating plasmid with sgRNA cassette containing a killing spacer #3 targeting the wild type polC gene.

   Depositor

   Lee Lynd

   Article

   Lanahan et al Appl Environ Microbiol. 2022 Jan 11;88(1

   Insert

   spacer expression cassette
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pOD_004

   Plasmid

   #155361

   Purpose

   Gentamycin version of the scarless genome engineering pDEL plasmid (Tikh et al. 2016) compatible with Salmonella

   Depositor

   Elisabeth Raleigh

   Article

   Zaworski et al PLoS Genet. 2022 Apr 4;18(4):e1009943. d

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 30, 2022

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

6. pRAF-MutL(R95F)

   Plasmid

   #86608

   Purpose

   E. coli Mutl(R95F) expression plasmid

   Depositor

   Richard Fishel

   Article

   Liu et al Nature. 2016 Nov 16;539(7630):583-587. d

   Insert

   E. coli MutL(R95F)
   

   Use

   Tags

   his-tag and ald-tag

   Expression

   Bacterial

   Mutation

   R95F

   Promoter

   T7

   Available since

   June 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. AP553‐1

   Plasmid

   #87240

   Purpose

   gtbp‐1 (~500 bp flanking sequence)::GFP (with 3 introns)::gtbp‐1 (~500 bp flanking sequence)

   Depositor

   Geraldine Seydoux

   Article

   Paix et al Nucleic Acids Res. 2016 Sep 6;44(15):e12

   Insert

   gtbp‐1 (~500 bp flanking sequence)::GFP (with 3 introns)::gtbp‐1 (~500 bp flanking sequence)
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAD06 K. lactis telomerase RNA hairpin

   Plasmid

   #61895

   Purpose

   Plasmid for run-off in vitro transcription of hairpin from the Kluyveromyces lactis telomerase RNA (nt 229-273)

   Depositor

   Thomas Cech

   Article

   Dalby et al RNA. 2013 Jun;19(6):841-51. doi: 10.1261

   Insert

   Telomerase RNA hairpin (nt 229-273)
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   T7

   Available since

   March 13, 2015

   Availability

   Academic Institutions and Nonprofits only

9. Cdk12_intron3_sg2_pX330

   Plasmid

   #127174

   Purpose

   sgRNA that cuts within intron 3 of mouse Cdk12 genomic locus in pX330 backbone

   Depositor

   Phil Sharp

   Article

   Dubbury et al Nature. 2018 Dec;564(7734):141-145. doi:

   Insert

   Mouse Cdk12 Intron 3 sgRNA
   

   Use

   CRISPR and Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   U6 Promoter

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

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10. Cdk12_intron4_sg1_pX458

    Plasmid

    #127175

    Purpose

    sgRNA that cuts within intron 4 of mouse CDK12 genomic locus in pX458 backbone

    Depositor

    Phil Sharp

    Article

    Dubbury et al Nature. 2018 Dec;564(7734):141-145. doi:

    Insert

    Mouse Cdk12 Intron 4 sgRNA
    

    Use

    CRISPR and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    U6 Promoter

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pYY13-URA3-TP901LP

    Plasmid

    #235772

    Purpose

    Plamids for generation of donor DNA for construction of a TP901-compatible landing pad p1 with URA3 selection marker.

    Depositor

    Chang Liu

    Article

    Pisera et al bioRxiv [Preprint]. 2024 Nov 30:2024.11.

    Insert

    URA3
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCIneo-hCHD7

    Plasmid

    #89458

    Purpose

    constitutive expression of human CHD7 in mammalian cells

    Depositor

    Peter Scacheri

    Insert

    CHD7 (CHD7 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. MLH1

    Plasmid

    #32859

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    MLH1 (MLH1 Human)
    

    Use

    Tags

    His (with TEV cleavage)

    Expression

    Bacterial

    Mutation

    contains amino acids 486-751

    Promoter

    Available since

    Feb. 22, 2012

    Availability

    Industry, Academic Institutions, and Nonprofits

14. TTN/pAS2-1

    Plasmid

    #98127

    Purpose

    titin in DNA-binding domain (BD) vector, used for yeast-two hybrid

    Depositor

    Rita Shiang

    Insert

    TTN (TTN Human)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Aug. 21, 2017

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

15. pLgw ELK1Y158A-V5-EcoDam

    Plasmid

    #98609

    Purpose

    Mammalian DamID lentiviral vector for fusing ELK1Y158A with Dam-V5 using Gateway cloning

    Depositor

    Richard Harvey

    Insert

    ELK1Y158A (Elk1 Mouse)
    

    Use

    Lentiviral; Damid

    Tags

    V5 and Dam (DNA adenine methyltransferase)

    Expression

    Mammalian

    Mutation

    Promoter

    Heat Shock Minimal Promoter

    Available since

    July 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLgw EcoDam-V5-ELK1Y158A

    Plasmid

    #98608

    Purpose

    Mammalian DamID lentiviral vector for fusing ELK1Y158A with Dam-V5 using Gateway cloning

    Depositor

    Richard Harvey

    Insert

    ELK1Y158A (Elk1 Mouse)
    

    Use

    Lentiviral; Damid

    Tags

    Dam (DNA adenine methyltransferase) and V5

    Expression

    Mammalian

    Mutation

    Promoter

    Heat Shock Minimal Promoter

    Available since

    July 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pLexA::nud-1

    Plasmid

    #11339

    Depositor

    Guy Caldwell

    Insert

    nud-1 (nud-1 Nematode)
    

    Use

    Tags

    LexA DNA Binding Domain

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 25, 2006

    Availability

    Academic Institutions and Nonprofits only

18. pLexA::lis-1

    Plasmid

    #11338

    Depositor

    Guy Caldwell

    Insert

    lis-1 (lis-1 Nematode)
    

    Use

    Tags

    LexA DNA Binding Domain

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 25, 2006

    Availability

    Academic Institutions and Nonprofits only

19. pCambia-dLB-kanR-GFP

    Plasmid

    #223177

    Purpose

    Plant Transformation Vector that allows marker removal through Mendelian inheritance. Start of DNA transfer at RB, terminates at left border but in ~1/3 of cases goes ahead until second left border

    Depositor

    Andreas Stuermer

    Type

    Empty backbone

    Use

    Ti plasmid for agrobacterium

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Aug. 26, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

20. pVax1-hVEGF165

    Plasmid

    #74466

    Purpose

    expression of hVEGF165 in mammalian cells

    Depositor

    Loree Heller

    Article

    Marshall WG et al Gene Ther. 2010 Mar;17(3):419-23. doi: 1

    Insert

    vascular endothelial growth factor 165 (VEGFA Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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