Addgene

We narrowed to 2,456 results for: Kap

Showing: 2061 - 2080 of 2456 results

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pLenti_CMV:Flag-OeNL

Plasmid

#208850

Purpose

Expresses FLAG epitope-tagged OeNL "Orange" BRET reporter in mammalian cells.

Depositor

Antonio Amelio

Article

Labra et al iScience. 2024 Aug 8;27(9):110655. doi:

Insert

OeNL Nanolantern

Use

Lentiviral

Tags

1xFLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 4, 2024

Availability

Academic Institutions and Nonprofits only

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FEMP

Plasmid

#191973

Purpose

FRET-based indicator of ER-mitochondria proximity (FEMP)

Depositor

Luca Scorrano

Article

Naon et al Proc Natl Acad Sci U S A. 2016 Oct 4;113

Insert

AkapYFP-Tav2a-CFP-sac1

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3 IKKe flag

Plasmid

#26201

Depositor

Tom Maniatis

Article

Fitzgerald et al Nat Immunol. 2003 May;4(5):491-6.

Insert

I-kappa-B kinase epsilon (IKBKE Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 24, 2011

Availability

Academic Institutions and Nonprofits only

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HA-TRIM28deltaLinker

Plasmid

#124958

Purpose

Mammalian expression of Human TRIM28 with deleted Linker region between CC and PHD domains

Depositor

Michelle Barton

Article

Li et al Oncogene. 2017 May 25;36(21):2991-3001.

Insert

hTRIM28 (TRIM28 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Deletion of linker region between CC domain and P…

Promoter

Available since

May 3, 2019

Availability

Academic Institutions and Nonprofits only

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GFP-Tog

Plasmid

#29480

Depositor

Linda Wordeman

Article

Moore et al J Cell Biol. 2005 May 9. 169(3):391-7.

Insert

ch-TOG (CKAP5 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

K19R, E118G, G254R, K1834R

Promoter

Available since

Oct. 5, 2011

Availability

Academic Institutions and Nonprofits only

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pET15b/bPanK

Plasmid

#73619

Purpose

Produces Escherichia coli pantothenate kinase, with N-terminal His6 tag (H6PanK)

Depositor

T. J. Kappock, Suzanne Jackowski

Article

Calder et al J Biol Chem. 1999 Jan 22;274(4):2014-20.

Insert

pantothenate kinase (coaA Escherichia coli)

Use

Tags

His6

Expression

Bacterial

Mutation

relative to MG1655 sequence, changes glutamate-5 …

Promoter

T7

Available since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Mito-CaNAR2

Plasmid

#64731

Purpose

Mitochondria-targeted FRET biosensor for monitoring calcineurin (CaN) phosphatase activity; targeted to cytosolic face of mitochondrial outer membrane.

Depositor

Jin Zhang

Article

Mehta et al eLife. 2014 Jul 23;3:e03765. doi: 10.755

Insert

CaNAR2

Use

Tags

Cerulean3, N-terminal 30 amino acids from DAKAP1,…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 21, 2015

Availability

Academic Institutions and Nonprofits only

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pAPM-D4 miR30-TRIM28 ts3

Plasmid

#115864

Purpose

TRIM28 knockdown

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

Insert

TRIM28 shRNA (TRIM28 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

SFFV

Available since

Jan. 9, 2019

Availability

Academic Institutions and Nonprofits only

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pAPM-D4 miR30-TRIM28 ts1

Plasmid

#115862

Purpose

TRIM28 knockdown

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

Insert

TRIM28 shRNA (TRIM28 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

SFFV

Available since

Sept. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pRK7 IKK2/IKKb

Plasmid

#26203

Depositor

Tom Maniatis

Article

Fitzgerald et al Nat Immunol. 2003 May;4(5):491-6.

Insert

I-kappa-B kinase beta (IKBKB Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 29, 2011

Availability

Academic Institutions and Nonprofits only
pAAV-hSyn-FRISZ-TM

Plasmid

#176886

Purpose

AAV transfer plasmid for cell-surface-localized FRISZ (via PDGFR TM) under hSyn promoter

Depositor

Huiwang Ai

Article

Wu et al Sci Adv. 2023 Mar;9(9):eadd2058. doi: 10

Insert

Ig kappa-FRISZ-PDGFR TM

Use

AAV

Tags

Expression

Mutation

Promoter

hSyn

Available since

Feb. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-Mito-CaNARi

Plasmid

#64851

Purpose

Mitochondria-targeted FRET biosensor for monitoring calcineurin (CaN) activation; targeted to cytosolic face of mitochondrial outer membrane.

Depositor

Jin Zhang

Article

Mehta et al eLife. 2014 Jul 23;3:e03765. doi: 10.755

Insert

CaNARi

Use

Tags

Cerulean, N-terminal 30 amino acids from DAKAP1, …

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 8, 2015

Availability

Academic Institutions and Nonprofits only

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pCS2-mCherry_SNAP_SNAPV_PACT_Dead

Plasmid

#173073

Purpose

Dead SNAP tag at the centrosome

Depositor

Heidi Hehnly

Article

Bucko et al Elife. 2019 Dec 24;8. pii: 52220. doi: 1

Insert

mCherry-SNAP-SNAP-Varied-PACT

Use

Tags

mCherry-SNAP-SNAP-Varied

Expression

Mammalian

Mutation

AA144 in both SNAP tags changed from a cysteine t…

Promoter

CMV IE94

Available since

Aug. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pSVE Grb2 myc 49L

Plasmid

#26085

Depositor

Bob Weinberg

Article

Egan et al Nature. 1993 May 6. 363(6424):45-51.

Insert

Grb2 P49L (GRB2 Human)

Use

Tags

myc

Expression

Mammalian

Mutation

P49L

Promoter

Available since

Oct. 18, 2010

Availability

Academic Institutions and Nonprofits only
ch-Tog

Plasmid

#28290

Depositor

Linda Wordeman

Article

Moore et al J Cell Biol. 2005 May 9. 169(3):391-7.

Insert

ch-TOG (CKAP5 Human)

Use

Tags

Expression

Bacterial and Mammalian

Mutation

K19R, E118G

Promoter

Available since

April 8, 2011

Availability

Academic Institutions and Nonprofits only

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pCDNA3 IKKe T7

Plasmid

#27238

Depositor

Tom Maniatis

Article

Fitzgerald et al Nat Immunol. 2003 May;4(5):491-6.

Insert

I-kappa-B kinase epsilon (IKBKE Human)

Use

Tags

T7

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 24, 2011

Availability

Academic Institutions and Nonprofits only
pSFG-TFII-I-GFP beta

Plasmid

#22200

Depositor

Ananda Roy

Article

Hakre et al Mol Cell. 2006 Oct 20. 24(2):301-8.

Insert

TFII-I (GTF2I Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 13, 2009

Availability

Academic Institutions and Nonprofits only

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eDrTrkA

Plasmid

#183996

Purpose

Optically controlled eDrTrkA expression in mammalian cells

Depositor

Vladislav Verkhusha, Dmitry Gerashchenko

Article

Leopold et al Nat Methods. 2022 Jul;19(7):871-880. doi

Insert

eDrTrkA (NTRK1 Human)

Use

Tags

Ig kappa secretory signal, mCherry, Deinococcus r…

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pSFG-TFII-I-GFP delta

Plasmid

#22190

Depositor

Ananda Roy

Article

Desgranges et al Mol Cell Biol. 2005 Dec . 25(24):10940-5

Insert

TFII-I (GTF2I Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 13, 2009

Availability

Academic Institutions and Nonprofits only

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pcDNA3.4_7C11-1 light chain

Plasmid

#198252

Purpose

recombinant expression of light chain of monoclonal anti-AMP antibody 7C11-1

Depositor

Aymelt Itzen

Article

Hopfner et al iScience. 2020 Nov 13;23(12):101800. doi

Insert

light chain of monoclonal anti-AMP antibody 7C11-1 (mouse kappa)

Use

Tags

IL10 signal peptide

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 5, 2023

Availability

Academic Institutions and Nonprofits only

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