We narrowed to 6,478 results for: sas

1. ssAAV-EF1a-FLuc-WPRE-HgHpA_bac_293

   Plasmid

   #118412

   Purpose

   Expresses firefly luciferase under the control of a strong ubiquitous promoter EF1a along with a WPRE cassette. Backbone is compatible with both baculoviral and human production methods of AAV.

   Depositor

   Nicole Paulk

   Article

   Rumachik et al Mol Ther Methods Clin Dev. 2020 May 22;1

   Insert

   Firefly luciferase
   

   Use

   AAV, Luciferase, and Synthetic Biology

   Tags

   Expression

   Insect and Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Nov. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLKO.1-puro-U6-AsCas12a-TLR-MCV1-gRNA

   Plasmid

   #117411

   Purpose

   AsCas12a-gRNA targeting TLR2.0

   Depositor

   Erik Sontheimer

   Article

   Iyer et al CRISPR J. 2022 Oct;5(5):685-701. doi: 10

   Insert

   AsCas12a gRNA targeting TLR 2.0
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Dec. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3-sACE2v2.4-IgG1

   Plasmid

   #149668

   Purpose

   Mammalian expression plasmid for soluble ACE2-Fc fusion (IgG1) high affinity 2.4

   Depositor

   Erik Procko

   Article

   Chan et al Science. 2020 Sep 4;369(6508):1261-1265.

   Insert

   Angiotensin-converting enzyme 2 (ACE2 Human)
   

   Use

   Tags

   Fc region of human IgG1

   Expression

   Mammalian

   Mutation

   Extracellular, soluble protease domain (a.a. M1-D…

   Promoter

   CMV

   Available since

   May 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLKO_AU1.HSV.S_mCherry-NLS

   Plasmid

   #178224

   Purpose

   Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.

   Depositor

   Brian Brown

   Article

   Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00

   Insert

   nPC Tagged mCherry-NLS
   

   Use

   Lentiviral

   Tags

   AU1.HSV.S and Nuclear Localization Signal

   Expression

   Mutation

   Promoter

   eF1a

   Available since

   Jan. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. NC15 pGLUE CUL3

   Plasmid

   #36970

   Depositor

   Randall Moon

   Article

   Camp et al J Biol Chem. 2012 Feb 24;287(9):6539-50.

   Insert

   CUL3 (CUL3 Human)
   

   Use

   Tags

   CBP tag, HA, and streptag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 10, 2012

   Availability

   Academic Institutions and Nonprofits only

6. pcDNA3-sACE2v2-sfGFP

   Plasmid

   #145173

   Purpose

   Mammalian expression plasmid for sACE2-sfGFP high affinity variant 2

   Depositor

   Erik Procko

   Article

   Chan et al Science. 2020 Sep 4;369(6508):1261-1265.

   Insert

   Angiotensin-converting enzyme 2 (ACE2 Human)
   

   Use

   Tags

   Linker GSGGSGSGG and superfolder GFP

   Expression

   Mammalian

   Mutation

   Extracellular, soluble protease domain (a.a. M1-D…

   Promoter

   CMV

   Available since

   April 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBS-f1(+)-SEC61B-mEGFP

   Plasmid

   #115910

   Purpose

   donor to insert EGFP at the SEC61B locus in human cells

   Depositor

   Erik Sontheimer

   Article

   Iyer et al CRISPR J. 2022 Oct;5(5):685-701. doi: 10

   Insert

   SEC61B-mEGFP (SEC61B Human)
   

   Use

   Donor template

   Tags

   EGFP

   Expression

   Mutation

   Promoter

   Available since

   March 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLKO_HA.NWS.Ollas_mCherry-NLS

   Plasmid

   #178263

   Purpose

   Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.

   Depositor

   Brian Brown

   Article

   Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00

   Insert

   nPC Tagged mCherry-NLS
   

   Use

   Lentiviral

   Tags

   HA.NWS.Ollas and Nuclear Localization Signal

   Expression

   Mutation

   Promoter

   eF1a

   Available since

   Jan. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pRK5 FLAG 17xS/T->A, catalytic dead lipin 1

   Plasmid

   #32008

   Depositor

   David Sabatini

   Article

   Peterson et al Cell. 2011 Aug 5;146(3):408-20.

   Insert

   Lipin1 (Lpin1 Mouse)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   D712E, D714E AND S106A, S150A, (S281A, T282A), S2…

   Promoter

   CMV, SP6

   Available since

   Oct. 18, 2011

   Availability

   Academic Institutions and Nonprofits only

10. pLKO_NWS.HA.V5_mCherry-NLS

    Plasmid

    #178283

    Purpose

    Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.

    Depositor

    Brian Brown

    Article

    Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00

    Insert

    nPC Tagged mCherry-NLS
    

    Use

    Lentiviral

    Tags

    NWS.HA.V5 and Nuclear Localization Signal

    Expression

    Mutation

    Promoter

    eF1a

    Available since

    Jan. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pD649-HAsp-SEMA3F-COMP5AP-AviTag-9xHis

    Plasmid

    #157541

    Purpose

    Mammalian expression of secreted protein fused to COMP5AP-AviTag-9xHis.

    Depositor

    Chris Garcia

    Article

    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

    Insert

    SEMA3F (SEMA3F Human)
    

    Use

    Tags

    COMP5AP-AviTag-9xHis

    Expression

    Mammalian

    Mutation

    Promoter

    CMV/SP6

    Available since

    March 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. AKAP1-EGFP-uTEV1

    Plasmid

    #171005

    Purpose

    HiLITR protease with AKAP1 N-terminal tmd targeting information (Mitochondria)

    Depositor

    Alice Ting

    Article

    Coukos et al eLife. 2021 Aug 20;10. pii: 69142. doi:

    Insert

    AKAP1(tmd)-EGFP-uTEV1 (AKAP1 Synthetic)
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    pTRE-tight (TetOn)

    Available since

    Aug. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3-sACE2v6-sfGFP

    Plasmid

    #145177

    Purpose

    Mammalian expression plasmid for sACE2-sfGFP high affinity variant 6

    Depositor

    Erik Procko

    Article

    Chan et al Science. 2020 Sep 4;369(6508):1261-1265.

    Insert

    Angiotensin-converting enzyme 2 (ACE2 Human)
    

    Use

    Tags

    Linker GSGGSGSGG and superfolder GFP

    Expression

    Mammalian

    Mutation

    Extracellular, soluble protease domain (a.a. M1-D…

    Promoter

    CMV

    Available since

    April 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pcDNA3-sACE2v2.4(732)

    Plasmid

    #154100

    Purpose

    Mammalian expression plasmid for dimeric soluble ACE2 high affinity variant 2.4 (untagged)

    Depositor

    Erik Procko

    Article

    Chan et al Science. 2020 Sep 4;369(6508):1261-1265.

    Insert

    Angiotensin-converting enzyme 2 (ACE2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Extracellular, soluble protease and neck domains …

    Promoter

    CMV

    Available since

    June 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pcDNA3-sACE2v1-sfGFP

    Plasmid

    #145172

    Purpose

    Mammalian expression plasmid for sACE2-sfGFP high affinity variant 1

    Depositor

    Erik Procko

    Article

    Chan et al Science. 2020 Sep 4;369(6508):1261-1265.

    Insert

    Angiotensin-converting enzyme 2 (ACE2 Human)
    

    Use

    Tags

    Linker GSGGSGSGG and superfolder GFP

    Expression

    Mammalian

    Mutation

    Extracellular, soluble protease domain (a.a. M1-D…

    Promoter

    CMV

    Available since

    April 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pLKO_AU1.VSVg.V5_mCherry-NLS

    Plasmid

    #178236

    Purpose

    Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.

    Depositor

    Brian Brown

    Article

    Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00

    Insert

    nPC Tagged mCherry-NLS
    

    Use

    Lentiviral

    Tags

    AU1.VSVg.V5 and Nuclear Localization Signal

    Expression

    Mutation

    Promoter

    eF1a

    Available since

    Jan. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pLKO_AU1.S.VSVg_mCherry-NLS

    Plasmid

    #178234

    Purpose

    Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.

    Depositor

    Brian Brown

    Article

    Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00

    Insert

    nPC Tagged mCherry-NLS
    

    Use

    Lentiviral

    Tags

    AU1.S.VSVg and Nuclear Localization Signal

    Expression

    Mutation

    Promoter

    eF1a

    Available since

    Jan. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDNA3-sACE2v2(732)-IgG1

    Plasmid

    #154105

    Purpose

    Mammalian expression plasmid for dimeric soluble ACE2 high affinity variant 2 (IgG1-Fc fusion)

    Depositor

    Erik Procko

    Article

    Chan et al Science. 2020 Sep 4;369(6508):1261-1265.

    Insert

    Angiotensin-converting enzyme 2 (ACE2 Human)
    

    Use

    Tags

    Fc region of human IgG1

    Expression

    Mammalian

    Mutation

    Extracellular, soluble protease and neck domains …

    Promoter

    CMV

    Available since

    June 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAdEasy-hALDOA

    Plasmid

    #210812

    Purpose

    To package adenovirus expressing wild-type aldolaseA

    Depositor

    Sheng-cai Lin

    Article

    Zhang et al Nature. 2017 Aug 3;548(7665):112-116. do

    Insert

    ALDOA (ALDOA Human)
    

    Use

    Adenoviral

    Tags

    Expression

    Mutation

    Promoter

    CMV

    Available since

    Dec. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pD649-HAsp-CEACAM5-COMP5AP-AviTag-9xHis

    Plasmid

    #157501

    Purpose

    Mammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.

    Depositor

    Chris Garcia

    Article

    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

    Insert

    CEACAM5 (CEACAM5 Human)
    

    Use

    Tags

    COMP5AP-AviTag-9xHis

    Expression

    Mammalian

    Mutation

    Promoter

    CMV/SP6

    Available since

    March 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge