We narrowed to 7,306 results for: GFP expression plasmids

1. pCJH065_proD_Cas12c_DualRNA-GFP+HDVrz_CAM

   Plasmid

   #183077

   Purpose

   Plasmid expressing Cas12c tracrRNA and GFP-targeting crRNA separately, with the crRNA ending with a HDV ribozyme sequence.

   Depositor

   Jennifer Doudna

   Article

   Huang et al Mol Cell. 2022 Jun 2;82(11):2148-2160.e4

   Insert

   Cas12c tracrRNA and crRNA ending with a HDV ribozyme
   

   Use

   CRISPR

   Expression

   Bacterial

   Available Since

   June 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pgRGFP

   Plasmid

   #82695

   Purpose

   Expresses gRNA of interest under U6 promoter (cloning by BpiI) and GFP under PGK promoter.

   Depositor

   Alan Mullen

   Article

   Daneshvar et al Cell Rep. 2016 Oct 4;17(2):353-365. doi:

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   PGK, U6

   Available Since

   Nov. 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCAG PSAM4-5HT3 HC IRES EGFP

   Plasmid

   #119740

   Purpose

   Chemogenetic activator expression plasmid

   Depositor

   Scott Sternson

   Article

   Magnus et al Science. 2019 Mar 14. pii: science.aav52

   Insert

   PSAM4 a75HT3 HC (homo) IRES eGFP (CHRNA7, HTR3A Human, Synthetic)
   

   Expression

   Mammalian

   Mutation

   L131G, Q139L, Y217F, R420Q, R424D, R428A

   Promoter

   CAG

   Available Since

   March 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-CMV-MCS-noATG-GFP

   Plasmid

   #223181

   Purpose

   A multicloning site (MCS) was synthesized and inserted into AAV-CMV-GFP to construct the AAV-CMV-MCS-(no ATG)-GFP negative control plasmid.

   Depositor

   Yuxuan Guo

   Article

   Chen et al Nat Cardiovasc Res. 2025 Jun 13. doi: 10

   Type

   Empty backbone

   Use

   AAV

   Available Since

   June 18, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-hSyn-DIO-Gluc-NHP.RMA-IRES-EGFP

   Plasmid

   #227887

   Purpose

   Expresses Gluc-NHP.RMA and EGFP in the presence of Cre recombinase. Double-floxed Gluc-NHP.RMA and EGFP for monitoring Cre-expressing neuronal populations.

   Depositor

   Jerzy Szablowski

   Article

   Lee et al bioRxiv 2025.06.01.657212; doi: 10.1101/

   Inserts

   Gaussia luciferase fused to macaque Fc
   IRES-EGFP
   

   Use

   AAV, Cre/Lox, and Luciferase

   Tags

   Gluc and IgG1 Fc

   Expression

   Mammalian

   Available Since

   June 4, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. 2X_pX458_pSpCas9(BB)-2A-GFP

   Plasmid

   #172221

   Purpose

   Cas9-2A-GFP expression vector bearing two (one additional) independent sgRNA cassettes.

   Depositor

   Alexander Meissner

   Article

   Wu et al Nat Commun. 2021 Aug 12;12(1):4897. doi:

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   3XFLAG and GFP

   Expression

   Mammalian

   Promoter

   Cbh

   Available Since

   Sept. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET51b(+)_EGFP_NbALFA

   Plasmid

   #136626

   Purpose

   Plasmid encoding the bacterial expression of NbALFA for ALFA-tag as an EGFP-fusion

   Depositor

   Kai Johnsson

   Article

   Farrants et al Nat Methods. 2020 Feb 17. pii: 10.1038/s

   Insert

   EGFP-NbALFA
   

   Tags

   Hisx10 and Strep

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Feb. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBAD-LSSmGFP

   Plasmid

   #186542

   Purpose

   LSSmGFP fluorescent protein (ex/em = 400/510 nm): bacterial expression plasmid

   Depositor

   Gregory Petsko

   Article

   Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

   Insert

   LSSmGFP
   

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   hfYFP-T43S/G65S/L68Q/H77N/K140N/Y203I/V206K

   Promoter

   araB

   Available Since

   Nov. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV.GFA104.PI.eGFP.WPRE.bGH

   Plasmid

   #100896

   Purpose

   AAV expression of EGFP in astrocytes

   Depositor

   Philip Haydon

   Article

   Ortinski et al Nat Neurosci. 2010 May;13(5):584-91. doi

   Has Service

   AAV5

   Insert

   EGFP
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   GFA104 (GFAP promoter)

   Available Since

   Jan. 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. RP2-EGFP

    Plasmid

    #86073

    Purpose

    A mammalian expression plasmid encoding retinitis pigmentosa 2 (RP2) with C-terminus EGFP.

    Depositor

    Lei Lu

    Article

    Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

    Insert

    RP2 (RP2 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLVUT-spGFP

    Plasmid

    #225486

    Purpose

    Tet-on lentiviral vector for controlled expression of secretable and permeable EGFP

    Depositor

    Robert Gross

    Article

    Gutekunst et al Neuroscience. 2016 Dec 17;339:308-318. d

    Insert

    Igκ leader and TAT peptide
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    Human ubiquitin C

    Available Since

    Sept. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. mEGFP-NbALFA

    Plasmid

    #159986

    Purpose

    For the expresson and purification of the EGFP-tagged anti-ALFA tag nanobody (NbALFA)

    Depositor

    Motoyuki Hattori

    Article

    Jin et al Commun Biol. 2021 Mar 19;4(1):366. doi:

    Insert

    mEGFP-NbALFA
    

    Tags

    8histidine-HRV3C protease cleavage site

    Expression

    Bacterial

    Mutation

    3GS linker between mEGFP and NbALFA

    Available Since

    Oct. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. PB_TRE_sg61-PDGFR_EF1a_eGFP

    Plasmid

    #186310

    Purpose

    Plasmid for inducible mammalian cell expression of the sg61 helixCAM and constitutive expression of an identifying fluorescent protein (eGFP). Flanked with PiggyBac sites for easy line construction

    Depositor

    George Church

    Article

    Chao et al Cell. 2022 Aug 23. pii: S0092-8674(22)01

    Inserts

    sg61-PDGFR
    eGFP
    

    Tags

    His, myc

    Expression

    Mammalian

    Available Since

    Aug. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. PB_TRE_Z18-PDGFR_EF1a_eGFP

    Plasmid

    #186304

    Purpose

    Plasmid for inducible mammalian cell expression of the Z18 helixCAM and constitutive expression of an identifying fluorescent protein (eGFP). Flanked with PiggyBac sites for easy line construction.

    Depositor

    George Church

    Article

    Chao et al Cell. 2022 Aug 23. pii: S0092-8674(22)01

    Inserts

    Z18-PDGFR
    eGFP
    

    Tags

    HA, myc

    Expression

    Mammalian

    Available Since

    Aug. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. PRS_8-eGFP

    Plasmid

    #192589

    Purpose

    AAV-vector for fluorescent labeling of noradrenergic neurons

    Depositor

    Simon Wiegert

    Article

    Wissing et al PLoS Biol. 2025 Jul 7;23(7):e3003228. do

    Insert

    EGFP
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    PRSX8

    Available Since

    Dec. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCS2_hMGFP-60A

    Plasmid

    #200393

    Purpose

    A plasmid IVT template containing a humanized Monster Green Fluorescent Protein (hMGFP) CDS followed by a template-encoded poly(A) tail. Linearization at an Esp3i site.

    Depositor

    Xiao Wang

    Article

    Aditham et al ACS Chem Biol. 2022 Dec 16;17(12):3352-3

    Insert

    monster GFP
    

    Expression

    Mammalian

    Available Since

    May 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-C+F-GFP

    Plasmid

    #209699

    Purpose

    Expresses GFP dependent on the combined activity of CRE- and FLP-recombinases

    Depositor

    Peer Wulff

    Article

    Rangel Guerrero et al Neuron. 2024 Apr 10:S0896-6273(24)00197-

    Insert

    GFP
    

    Use

    AAV

    Promoter

    CBA

    Available Since

    Jan. 8, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV-Syn-GFP

    Plasmid

    #58867

    Purpose

    AAV expression of GFP under the Syn promoter

    Depositor

    Edward Boyden

    Article

    Boyden et al Nat Neurosci. 2005 Sep . 8(9):1263-8.

    Insert

    GFP
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    Syn

    Available Since

    Aug. 14, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLVX-PERK(LD)-EGFP

    Plasmid

    #225680

    Purpose

    Used to generate stable cell lines that express PERK(LD)-EGFP. This plasmid serves as a control for pLVX-PERK(LD)-EGFP-HOTag3.

    Depositor

    Likun Wang

    Article

    Xu et al Cell Rep. 2024 Jun 25;43(6):114358. doi:

    Type

    Empty backbone

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Oct. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. AAV-hGFAP-GFP

    Plasmid

    #178580

    Purpose

    Expression of GFP under the human GFAP promoter

    Depositor

    Chun-Li Zhang

    Article

    Wang et al Cell. 2021 Oct 14;184(21):5465-5481.e16.

    Insert

    GFP
    

    Use

    AAV

    Expression

    Mammalian

    Available Since

    Feb. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge