We narrowed to 2,475 results for: T2A

1. pCDH-EF1a-hSNCA

   Plasmid

   #170445

   Purpose

   Lentiviral vector expressing human SNCA, under control of EF1alpha promoter.

   Depositor

   Sang-Hun Lee

   Article

   Wulansari et al Sci Adv. 2021 Feb 17;7(8). pii: 7/8/eabb

   Insert

   human SNCA (SNCA Human)
   

   Use

   Lentiviral

   Promoter

   EF1a

   Available Since

   June 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-CAG-mCherry-Tandem-PSAM4-GlyR

   Plasmid

   #208361

   Purpose

   Expression of the inhibitory chemogenetic tool PSAM4-GlyR and mCherry reporter. Application of ultrapotent agonists induces neuronal silencing.

   Depositor

   David Bennett

   Article

   Perez-Sanchez et al Sci Transl Med. 2023 Oct 4;15(716):eadh3

   Insert

   mCherry-TANDEM-PSAM4-GlyR
   

   Use

   AAV

   Tags

   mCherry

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Oct. 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pWZ853

   Plasmid

   #208115

   Purpose

   mSwAP-In marker cassette 2 with 5’PB-mNeongreen-HPRT1-BSD

   Depositor

   Jef Boeke

   Article

   Zhang et al Nature. 2023 Nov 1. doi: 10.1038/s41586-

   Insert

   mNeonGreen-HPRT1-BSD
   

   Tags

   T2A

   Expression

   Bacterial and Mammalian

   Promoter

   EF1alpha

   Available Since

   Nov. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTet-O-Nd1-puro

   Plasmid

   #52052

   Purpose

   Expresses Nd1 and puromycin resistent gene under control of TetON promoter

   Depositor

   Marius Wernig

   Article

   Zhang et al Neuron. 2013 Jun 5;78(5):785-98. doi: 10

   Insert

   Neurod1 (Neurod1 Mouse)
   

   Use

   Lentiviral

   Tags

   T2A-and puromycin resistant cassette

   Expression

   Mammalian

   Promoter

   tet-on

   Available Since

   April 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pPN441

   Plasmid

   #137873

   Purpose

   PiggyBac vector for expression of 3xgRNA targeting TCF4 for CRISPRi; mRFP-T2A-BlasticidinR

   Depositor

   Lindy Barrett

   Article

   Hazelbaker et al Sci Rep. 2020 Jan 20;10(1):635. doi: 10.

   Insert

   TCF4 (TCF4 Human)
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Feb. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. PB-U6-CNCB_sgEIF3D-4

   Plasmid

   #236766

   Purpose

   A piggybac-based vector containing mouse U6 promoter-driven EIF3D sgRNA #4 and CAG promoter-driven nuclear-localized Clover-T2A-BSR.

   Depositor

   Kazutoshi Takahashi

   Article

   Okubo et al Sci Adv. 2025 Apr 11;11(15):eadq5484. do

   Insert

   EIF3D sgRNA-4 (EIF3D Human)
   

   Use

   Piggybac

   Expression

   Mammalian

   Promoter

   mouse U6 promoter and mouse U6 promoter, CAG prom…

   Available Since

   May 22, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. PA-Dre

   Plasmid

   #169706

   Purpose

   Photoactivatable Dre recombinase for AAV production and expression in mammalian cells

   Depositor

   Dali Li

   Article

   Li et al Proc Natl Acad Sci U S A. 2020 Dec 29;11

   Insert

   Dre246-nMag-T2A-pMag-Dre247
   

   Use

   AAV

   Expression

   Mammalian

   Mutation

   none

   Promoter

   CAG

   Available Since

   May 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLenti-H3.3(WT)-3AID-HA-2A-mTurquoiseBSD

   Plasmid

   #225701

   Purpose

   Lentiviral expression of AID degron tagged Wildtype H3.3 and mTurquoise fused BlasticidinR

   Depositor

   Lacra Bintu

   Article

   Sinha et al bioRxiv [Preprint]. 2023 Oct 14:2023.10.

   Insert

   H3.3 (WT) (H3-3A, H3-3B Human)
   

   Use

   Lentiviral

   Tags

   3xAID HA and T2A-mTurquoise

   Expression

   Mammalian

   Available Since

   Oct. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pWZ854

   Plasmid

   #208116

   Purpose

   mSwAP-In marker cassette 2 with 5’PB-mNeongreen-HPRT1-Neo

   Depositor

   Jef Boeke

   Article

   Zhang et al Nature. 2023 Nov 1. doi: 10.1038/s41586-

   Insert

   mNeonGreen-HPRT1-Neo
   

   Tags

   T2A

   Expression

   Bacterial and Mammalian

   Promoter

   EF1alpha

   Available Since

   Nov. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pWZ852

    Plasmid

    #208114

    Purpose

    mSwAP-In marker cassette 1 with mScarlet-HygTK

    Depositor

    Jef Boeke

    Article

    Zhang et al Nature. 2023 Nov 1. doi: 10.1038/s41586-

    Insert

    mScarlet-HygTK
    

    Tags

    T2A

    Expression

    Bacterial and Mammalian

    Promoter

    EF1alpha

    Available Since

    Nov. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. Lenti-pNFkB::Cre-mRuby3

    Plasmid

    #164137

    Purpose

    Lentiviral construct for building stable cell lines expressing Cre recombinase and mRuby3 in the presence of TNFa

    Depositor

    Hyongbum Kim

    Article

    Park et al Cell. 2021 Jan 28. pii: S0092-8674(21)00

    Insert

    NFkB promoter-Cre-T2A-mRuby3
    

    Use

    CRISPR, Cre/Lox, Lentiviral, and Synthetic Biolog…

    Expression

    Mammalian

    Promoter

    NFkB synthetic promoter

    Available Since

    Feb. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. Lenti-pHSE::Cre-mRuby3

    Plasmid

    #164136

    Purpose

    Lentiviral construct for building stable cell lines expressing Cre recombinase and mRuby3 in the presence of heat induction

    Depositor

    Hyongbum Kim

    Article

    Park et al Cell. 2021 Jan 28. pii: S0092-8674(21)00

    Insert

    HSE promoter-Cre-T2A-mRuby3
    

    Use

    CRISPR, Cre/Lox, Lentiviral, and Synthetic Biolog…

    Expression

    Mammalian

    Promoter

    HSE synthetic promoter

    Available Since

    Feb. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. PEA1-Nuclease-Puro

    Plasmid

    #171992

    Purpose

    Delivers all prime editing nuclease components in a single, puromycin selectable plasmid

    Depositor

    Paul Thomas

    Article

    Adikusuma et al Nucleic Acids Res. 2021 Sep 17. pii: 637

    Insert

    CbH-Cas9-RT-T2A-Puro, hU6-pegRNA (Bbs1 gate), hU6-sgRNA (Bbs1 gate)
    

    Expression

    Mammalian

    Mutation

    GC to CA point mutation in SpCas9 to restore nucl…

    Promoter

    CMV for Cas9, U6 for gRNAs

    Available Since

    Aug. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. PEA1-Nuclease-GFP

    Plasmid

    #171994

    Purpose

    Delivers all prime editing nuclease components in a single, GFP selectable plasmid

    Depositor

    Paul Thomas

    Article

    Adikusuma et al Nucleic Acids Res. 2021 Sep 17. pii: 637

    Insert

    CbH-Cas9-RT-T2A-GFP, hU6-pegRNA (Bbs1 gate), hU6-sgRNA (Bbs1 gate)
    

    Expression

    Mammalian

    Mutation

    GC to CA point mutation in SpCas9 to restore nucl…

    Promoter

    CMV for Cas9, U6 for gRNAs

    Available Since

    Aug. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pPN440

    Plasmid

    #137874

    Purpose

    PiggyBac vector for expression of 3xgRNA targeting TCF4 for CRISPRa; mRFP-T2A-BlasticidinR

    Depositor

    Lindy Barrett

    Article

    Hazelbaker et al Sci Rep. 2020 Jan 20;10(1):635. doi: 10.

    Insert

    TCF4 (TCF4 Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Feb. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pRetroX-TRE3G-PLOD2_PGK-GpNLuc

    Plasmid

    #136457

    Purpose

    Expresses Tet/Dox-inducible human PLOD2 in mammalian cells.

    Depositor

    Antonio Amelio

    Article

    Sato et al Neoplasia. 2021 Jun 6;23(6):594-606. doi

    Insert

    TRE3G::PLOD2-T2A-Hygromycin (PLOD2 Human)
    

    Use

    Luciferase and Retroviral

    Expression

    Mammalian

    Promoter

    TRE3G

    Available Since

    June 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pPN458

    Plasmid

    #137876

    Purpose

    PiggyBac vector for expression of 1x gRNA targeting TCF4 for CRISPRa;mRFP-T2A-BlasticidinR

    Depositor

    Lindy Barrett

    Article

    Hazelbaker et al Sci Rep. 2020 Jan 20;10(1):635. doi: 10.

    Insert

    TCF4 (TCF4 Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Feb. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. CALID

    Plasmid

    #169708

    Purpose

    Cre-activated light-inducible Dre recombinase

    Depositor

    Dali Li

    Article

    Li et al Proc Natl Acad Sci U S A. 2020 Dec 29;11

    Insert

    a double-floxed inverted Dre246-nMag-T2A-pMag-Dre247
    

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CAG

    Available Since

    May 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. LoxP2-Mb2Tomato-2A (SO240)

    Plasmid

    #99614

    Purpose

    To clone gene of interest downstream of LoxP2-Mb2Tomato-2A cassette

    Depositor

    Rui Benedito

    Article

    Pontes-Quero et al Cell. 2017 Aug 10;170(4):800-814.e18. do

    Insert

    Mb2Tomato
    

    Tags

    T2A

    Expression

    Mammalian

    Available Since

    Sept. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. LoxP1-His-H2B-Cherry-2A (SO290)

    Plasmid

    #99616

    Purpose

    To clone gene of interest downstream of LoxP1-His-H2B-Cherry-2A cassette

    Depositor

    Rui Benedito

    Article

    Pontes-Quero et al Cell. 2017 Aug 10;170(4):800-814.e18. do

    Insert

    6xHis-H2B-Cherry
    

    Tags

    T2A

    Expression

    Mammalian

    Available Since

    Sept. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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