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EasyFusion mCherry

Plasmid

#112849

Purpose

General donor vector with mCherry coding sequence flanking by restriction sites for generating knock-in donor

Depositor

Janet Rossant

Article

Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

Insert

mCherry

Use

Synthetic Biology

Promoter

No

Available Since

July 26, 2018

Availability

Academic Institutions and Nonprofits only

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BTV

Plasmid

#84771

Purpose

bidirectional tetracycline-regulated viral reporter that measures the effects of 3' UTR sequences on mRNA and protein production

Depositor

David Erle

Article

Zhao et al Nat Biotechnol. 2014 Apr;32(4):387-91. d

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available Since

Oct. 28, 2019

Availability

Academic Institutions and Nonprofits only

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EasyFusion eGFP

Plasmid

#112848

Purpose

General donor vector with EGFP coding sequence flanking by restriction sites for generating knock-in donor

Depositor

Janet Rossant

Article

Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

Insert

EGFP

Use

Unspecified; General donor

Promoter

No

Available Since

July 26, 2018

Availability

Academic Institutions and Nonprofits only

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pSEVA1213S

Plasmid

#122095

Purpose

constitutive expression of I-SceI for genome engineering in Pseudomonas

Depositor

Pablo Ivan Nikel

Article

Wirth et al Microb Biotechnol. 2019 Mar 12. doi: 10.

Insert

I-SceI restriction endonuclease

Available Since

May 16, 2019

Availability

Academic Institutions and Nonprofits only

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pMAL-BRT17

Plasmid

#45358

Depositor

Scott Banta

Article

Shur et al Biotechniques. 2013 Apr;54(4):197-206. d

Insert

17 Repeat Beta Roll Tag

Tags

Maltose Binding Protein (MBP)

Expression

Bacterial

Promoter

Ptac

Available Since

June 11, 2013

Availability

Academic Institutions and Nonprofits only

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pADS

Plasmid

#19040

Depositor

Jay Keasling

Article

Martin et al Nat Biotechnol. 2003 Jul . 21(7):796-802

Insert

codon-optimized synthetic amorphadiene synthase

Use

Synthetic Biology

Expression

Bacterial

Available Since

Sept. 8, 2008

Availability

Academic Institutions and Nonprofits only
RCAS cVax (CC#337)

Plasmid

#15136

Depositor

Connie Cepko

Article

Schulte et al Neuron. 1999 Nov . 24(3):541-53.

Insert

cVax (VAX1 Chicken)

Use

Retroviral; Avian expression

Available Since

June 15, 2007

Availability

Academic Institutions and Nonprofits only
p8.9 NdeltaSB P90A

Plasmid

#132930

Purpose

p8.9 NdeltaSB bearing P90A mutant capsid sequence

Depositor

Jeremy Luban

Article

De Iaco et al Retrovirology. 2011 Dec 6;8:98. doi: 10.

Insert

gag P90A and pol

Use

Lentiviral

Mutation

P90A

Available Since

Oct. 4, 2019

Availability

Academic Institutions and Nonprofits only

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GsI-IIC-RT

Plasmid

#181806

Purpose

GsI-IIC RT

Depositor

Erik Sontheimer

Article

Liu et al Nat Biotechnol. 2022 Apr 4. pii: 10.1038

Insert

GsI-IIC-RT

Expression

Mammalian

Available Since

April 4, 2022

Availability

Academic Institutions and Nonprofits only

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pMK-N. tabacum trnI/trnA

Plasmid

#123579

Purpose

MoChlo kit

Depositor

Scott Lenaghan, C. Neal Stewart

Article

Occhialini et al Plant Physiol. 2019 Jan 24. pii: pp.18.0

Insert

N. tabacum trnI/trnA integration site

Use

Chloroplast transformation vector

Expression

Plant

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pMAL-c2E

Plasmid

#75291

Purpose

Expresses proteins in the cytoplasm as fusions to maltose-binding protein

Depositor

Paul Riggs

Article

Walker et al Appl Microbiol Biotechnol. 2010 Sep;88(1

Type

Empty backbone

Tags

maltose-binding protein

Expression

Bacterial

Promoter

tac

Available Since

July 20, 2016

Availability

Industry, Academic Institutions, and Nonprofits

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pSplit-NO-Dib1

Plasmid

#58786

Purpose

Express LucN-hDib1 fusion protein in mammalian cells

Depositor

Hiroyoshi Ariga

Article

Maita et al Anal Biochem. 2014 Feb 3. pii: S0003-269

Insert

hDIb1

Tags

Flag and Luciferase (1-398aa)

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 15, 2014

Availability

Academic Institutions and Nonprofits only
pCAG-mRuby2

Plasmid

#89686

Purpose

pCAG promoter driving mRuby2

Depositor

Wilson Wong

Article

Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

Insert

mRuby2

Use

Synthetic Biology

Expression

Mammalian

Available Since

April 27, 2017

Availability

Academic Institutions and Nonprofits only

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P-BT3332

Plasmid

#188110

Purpose

pBolux harboring the promoter region upstream of BT3332

Depositor

Guy Townsend

Article

Modesto et al Nat Commun. 2023 Jan 17;14(1):275. doi:

Insert

The B. thetaiotaomicron BT3332 promoter cloned into pBolux

Expression

Bacterial

Promoter

BT3332

Available Since

Jan. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pMM107

Plasmid

#127227

Purpose

35S::IPT::pinII terminator

Depositor

Daniel Voytas

Article

Maher et al Nat Biotechnol. 2019 Dec 16. pii: 10.103

Insert

IPT

Expression

Plant

Mutation

codon optimized CDSs

Available Since

Dec. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pBL132

Plasmid

#104333

Purpose

Expresses taz from a LacI regulated promoter under inducible IPTG control.

Depositor

Jeffrey Tabor

Article

Landry et al Nat Commun. 2018 Apr 12;9(1):1433. doi:

Insert

Taz

Expression

Bacterial

Promoter

PtacI

Available Since

April 17, 2018

Availability

Academic Institutions and Nonprofits only

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pLenti-EF1a-SOX2

Plasmid

#35388

Depositor

Feng Zhang

Article

Zhang et al Nat Biotechnol. 2011 Feb;29(2):149-53. d

Insert

TALE-Sox2

Use

Lentiviral

Expression

Mammalian

Available Since

March 29, 2012

Availability

Academic Institutions and Nonprofits only
pMT-mCherry-Patronin

Plasmid

#31753

Depositor

Ron Vale

Article

Goodwin et al Cell. 2010 Oct 15;143(2):263-74.

Insert

Patronin (Patronin Fly)

Tags

mCherry

Expression

Insect

Promoter

Metallothionein

Available Since

Sept. 21, 2011

Availability

Academic Institutions and Nonprofits only
GGE177

Plasmid

#120766

Purpose

Part of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Terminator of gene expression for position 1 (T1_Xpr2)

Depositor

Tristan Rossignol

Article

Larroude et al Microb Biotechnol. 2019 May 31. doi: 10.

Insert

T1_Xpr2

Use

Cloning/assembly plasmid

Available Since

Nov. 12, 2019

Availability

Academic Institutions and Nonprofits only

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p5419

Plasmid

#168186

Purpose

bacteria reporter

Depositor

Michael Hensel

Article

Schulte et al Commun Biol. 2021 May 4;4(1):520. doi: 1

Insert

PBAD::tag-rfp-T PtetA::sfgfp

Expression

Bacterial

Mutation

none

Available Since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

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