We narrowed to 15,806 results for: nans

1. pLyGo-Ec-1

   Plasmid

   #163127

   Purpose

   pLyGo cloning vector for cytoplasmic expression in E. coli of a sequence of interest (LPMO). Vector encoding Ubiquitin with an internal His10-tag and the LyGo cassette (SapI-ccdB-SapI)

   Depositor

   Morten Norholm

   Article

   Hernandez-Rollan et al ACS Synth Biol. 2021 Apr 16;10(4):897-90

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Ubiquitin

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   May 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLyGo-Bs-1

   Plasmid

   #163137

   Purpose

   pLyGo cloning vector for a sequence of interest (LPMO) in Bacillus subtilis. Vector encoding the AmyL signal peptide and the LyGo casette (SapI-ccdB-SapI)

   Depositor

   Morten Norholm

   Article

   Hernandez-Rollan et al ACS Synth Biol. 2021 Apr 16;10(4):897-90

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PamyL-PamyQ-PcryIIIA-cryIIIAstab49

   Available since

   May 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLyGo-Bs-3

   Plasmid

   #163141

   Purpose

   pLyGo cloning vector for a sequence of interest (LPMO) in B. subtilis. Vector encoding the AprE signal peptide and the LyGo cassette (SapI-ccdB-SapI)

   Depositor

   Morten Norholm

   Article

   Hernandez-Rollan et al ACS Synth Biol. 2021 Apr 16;10(4):897-90

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PamyL-PamyQ-PcryIIIA-cryIIIAstab49

   Available since

   May 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLyGo-Bs-2

   Plasmid

   #163140

   Purpose

   pLyGo cloning vector for a sequence of interest (LPMO) in Bacillus subtilis. Vector encoding the AmyQ signal peptide and the LyGo cassette (SapI-ccdB-SapI)

   Depositor

   Morten Norholm

   Article

   Hernandez-Rollan et al ACS Synth Biol. 2021 Apr 16;10(4):897-90

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PamyL-PamyQ-PcryIIIA-cryIIIAstab49

   Available since

   May 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLyGo-Ec-3

   Plasmid

   #163129

   Purpose

   pLyGo cloning vector for periplasmic expression in E. coli of a sequence of interest (LPMO). Vector encoding OmpA signal peptide and the LyGo cassette (SapI-ccdB-SapI)

   Depositor

   Morten Norholm

   Article

   Hernandez-Rollan et al ACS Synth Biol. 2021 Apr 16;10(4):897-90

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   May 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pLyGo-Ec-5

   Plasmid

   #163131

   Purpose

   pLyGo cloning vector for periplasmic expression in E. coli of a sequence of interest (LPMO). Vector encoding DsBA signal peptide and the LyGo cassette (SapI-ccdB-SapI)

   Depositor

   Morten Norholm

   Article

   Hernandez-Rollan et al ACS Synth Biol. 2021 Apr 16;10(4):897-90

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   April 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET28a-Coh-ddFLN4-ELP(MV7E2)3-His-ybbR

   Plasmid

   #157672

   Purpose

   Expression of Coh (type III from Ruminococcus flavefaciens) with ddFLN4, ELP linker, His6x tag, and ybbR Tag.

   Depositor

   Michael Nash

   Article

   Yang et al Nano Lett. 2020 Nov 16. doi: 10.1021/acs

   Insert

   Cohesin (R.f) with ddFLN4, ELP, Histag and ybbR tag
   

   Use

   Tags

   ddFLN4, ELP, Histag and ybbR tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Feb. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pUASTLOTattB_vhhGFP4::Nrv1::tagBFP

   Plasmid

   #163925

   Purpose

   Expression of GrabFP-B-extracellular under control of UAS or LOP enhancer

   Depositor

   Markus Affolter

   Article

   Harmansa et al Elife. 2017 Apr 11;6. doi: 10.7554/eLife

   Insert

   Fusion of vhhGPF4 (extracellular) nanobody with Nrv1 protein and tagBFP fluorophor
   

   Use

   Cre/Lox

   Tags

   tagBFP

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Jan. 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. K8.1Pr-pGL4.16-OriLyt

   Plasmid

   #131038

   Purpose

   Firefly Luciferase reporter for K8.1 promoter from KSHV with the lytic origin of replication

   Depositor

   Britt Glaunsinger

   Article

   Nandakumar et al PLoS Pathog. 2019 May 16;15(5):e1007774.

   Insert

   KSHV Lytic Origin of replication
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pFB-U6-control-gRNA-hSyn-mCherry-WPRE-SV40pA

    Plasmid

    #128347

    Purpose

    pAAV encoding control gRNA for CRISPR gRNAs listed above

    Depositor

    Ryan Drenan

    Article

    Peng et al Eur J Neurosci. 2018 May 19. doi: 10.111

    Insert

    control (negative)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pre_IV_CS10_1033_v1

    Plasmid

    #126860

    Purpose

    4x 1033 b long scaffolds divided by Zn-dependent self-cleaving DNAzyme cassettes, from compatibility group 10, made by method IV, yields linear 1033 b long scaffolds after Zn incubation

    Depositor

    Hendrik Dietz

    Article

    Engelhardt et al ACS Nano. 2019 Apr 22. doi: 10.1021/acsn

    Insert

    IV_CS10_1033_v1
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pNOC-CRISPR-compact

    Plasmid

    #99369

    Purpose

    Expresses Cas9-Nlux and sgRNA. Contains CEN/ARS. No selection marker.

    Depositor

    Eva Farre

    Article

    Poliner et al ACS Synth Biol. 2018 Apr 20;7(4):962-968

    Type

    Empty backbone

    Use

    Algae, nannochloropsis expression

    Tags

    Cas9-Nlux

    Expression

    Mutation

    Promoter

    Ribi promoter

    Available since

    Feb. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMito-mCherry-FRB_IRES_FKBP(III)-GBPen

    Plasmid

    #128268

    Purpose

    Bicistronic vector to express mitochondrial targeted mCherry-FRB (MitoTrap) as well as 3x FKBP-GBPenhancer (GFP Nanobody with 3 FKBP domains).

    Depositor

    Stephen Royle

    Article

    Kuey et al J Cell Sci. 2019 Nov 6;132(21). pii: jcs

    Inserts

    MitoTrap
    FKBP(III)-GBPen
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and IRES

    Available since

    Aug. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLenti-SF3B1-K700E-reporter

    Plasmid

    #200318

    Purpose

    A fluorescent reporter for SF3B1 K700E mutation. Co-expresses a blasticidin resistance gene for selection. Includes LoxP sites for Cre-mediated deletion of reporter cassette.

    Depositor

    Lili Wang

    Article

    Fernandez et al Cancer Res Commun. 2024 Aug 28. doi: 10.

    Insert

    BSD-2A-EGFPi
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1-a

    Available since

    Aug. 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pFB-U6-sgNeuN-gRNA-hSyn-mCherry-WPRE-SV40pA

    Plasmid

    #128346

    Purpose

    pAAV encoding control gRNA for CRISPR gRNAs listed above

    Depositor

    Ryan Drenan

    Article

    Peng et al Eur J Neurosci. 2018 May 19. doi: 10.111

    Insert

    Rbfox3 (NeuN) (Rbfox3 Mouse)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. 6xHis-SNAP-PI(3)P Probe

    Plasmid

    #211508

    Purpose

    For expression of recombinant biosensor for detection of Phosphatidylinositol-3-Phosphate (PI(3)P) from bacteria

    Depositor

    Hannes Maib, David Murray

    Article

    Maib et al J Cell Biol. 2024 Jun 3;223(6):e20231009

    Insert

    FYVE-Hrs x 2 (PIKFYVE Human)
    

    Use

    Tags

    6xhis-SNAP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLIX403_Capture1_APOBEC_HA_P2A_mRuby

    Plasmid

    #183901

    Purpose

    Inducible lentiviral expression, TRE-gateway-APOBEC-HA-P2A-mRuby; PGK-puro-2A-rtTA

    Depositor

    Eugene Yeo

    Article

    Brannan et al Nat Methods. 2021 May;18(5):507-519. doi

    Type

    Empty backbone

    Use

    Lentiviral; Gateway destination vector, doxycycli…

    Tags

    Capture1_APOBEC_HA_P2A_mRuby

    Expression

    Mammalian

    Mutation

    Promoter

    TRE promoter, Tet ON

    Available since

    Oct. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET21-pelB-LaG94-5

    Plasmid

    #228965

    Purpose

    For bacterial expression of anti-GFP nanobody LaG94-5, with pelB leader for periplasmic secretion, and C-term free cysteine and 6xHIS tag.

    Depositor

    Michael Rout

    Article

    Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

    Insert

    LaG94-5
    

    Use

    Tags

    6xHIS

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Jan. 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. RFP-(SIM)3

    Plasmid

    #127148

    Purpose

    Mammalian expression plasmid for mCherry-(SIM)3 client to assess SUMO-SIM scaffolds.

    Depositor

    Michael Rosen

    Article

    Banani et al Cell. 2016 Jul 28;166(3):651-663. doi: 1

    Insert

    3 repeats of human SUMO interactive motif (SIM) from PIASx each separated by (GGS)4 (PIAS2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAB1678 CMV-HIVNES-GS-Cas13bt1

    Plasmid

    #176316

    Purpose

    CMV-HIVNES-GS-Cas13bt1 (human codon optimized Cas13bt1 expression)

    Depositor

    Feng Zhang

    Article

    Kannan et al Nat Biotechnol. 2021 Aug 30. pii: 10.103

    Insert

    human codon optimized Cas13bt1
    

    Use

    CRISPR

    Tags

    HIV NES

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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