We narrowed to 2,307 results for: pET28

1. pDL17_Cas9-His Δcys M1C

   Plasmid

   #206251

   Purpose

   Bacterial expression of SpCas9 Δcys M1C variant under T7 promoter

   Depositor

   Amit Choudhary

   Article

   Lim et al Nat Commun. 2020 Aug 13;11(1):4043. doi:

   Insert

   SpCas9
   

   Use

   CRISPR

   Tags

   6x-His and SV40 NLS

   Expression

   Bacterial

   Mutation

   M1C, C80S, C574S

   Promoter

   T7

   Available since

   Sept. 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. Human RNF144B (27-236)

   Plasmid

   #110753

   Purpose

   Bacterial Expression of RNF144B RBR domain

   Depositor

   David Komander

   Article

   Michel et al Mol Cell. 2017 Oct 5;68(1):233-246.e5. d

   Insert

   RNF144B (RNF144B Human)
   

   Use

   Tags

   N-His6-GST-3C

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   June 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. AMPKA2

   Plasmid

   #36194

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   AMPKA2 (PRKAA2 Human)
   

   Use

   Tags

   His tag with thrombin cleavage

   Expression

   Bacterial

   Mutation

   contains KINASE DOMAIN only

   Promoter

   Available since

   Aug. 8, 2012

   Availability

   Industry, Academic Institutions, and Nonprofits

4. pJBL727

   Plasmid

   #140397

   Purpose

   Expresses CsoR protein under the control of T7-lacO promoter

   Depositor

   Julius Lucks

   Article

   Jung et al Nat Biotechnol. 2020 Jul 6. pii: 10.1038

   Insert

   T7-lacO-CsoR-T
   

   Use

   Tags

   6XHis-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 8, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. 6x-His_A1aY1_GFP

   Plasmid

   #158753

   Purpose

   Bacterial expression of 6xHis-A1aY1 fused with carboxy terminus GFP for recombinant purification

   Depositor

   Minhajuddin Sirajuddin

   Article

   Kesarwani et al J Cell Biol. 2020 Oct 5;219(10). pii: 15

   Insert

   6xHistidine_A1aY1_GFP
   

   Use

   Tags

   6x-Histidine tag, GFP, T7 leader sequence, and Th…

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Sept. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. Fast-GCaMP-EF20

   Plasmid

   #52645

   Purpose

   genetically encodable fluorescent calcium indicator protein to monitor neuronal activity

   Depositor

   Samuel Wang

   Article

   Sun et al Nat Commun. 2013 Jul 17;4:2170. doi: 10.

   Insert

   Fast-GCaMP-EF20
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   D362N/D366N mutated from GCaMP3

   Promoter

   T7

   Available since

   April 23, 2014

   Availability

   Academic Institutions and Nonprofits only

7. pJBL725

   Plasmid

   #140395

   Purpose

   Expresses SmtB protein under the control of T7-lacO promoter

   Depositor

   Julius Lucks

   Article

   Jung et al Nat Biotechnol. 2020 Jul 6. pii: 10.1038

   Insert

   T7-lacO-SmtB-T
   

   Use

   Tags

   TEV-6XHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 8, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. HTATIP (2OU2)

   Plasmid

   #33338

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   HTATIP (KAT5 Human)
   

   Use

   Tags

   Hexahistidine tag with Thrombin cleavage

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Jan. 11, 2012

   Availability

   Industry, Academic Institutions, and Nonprofits

9. pET-NEMO

   Plasmid

   #11966

   Depositor

   Jon Ashwell

   Article

   Wu et al Nat Cell Biol. 2006 Apr . 8(4):398-406.

   Insert

   NEMO (IKBKG Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 13, 2006

   Availability

   Academic Institutions and Nonprofits only

10. Fast-GCaMP-EF16

    Plasmid

    #52642

    Purpose

    genetically encodable fluorescent calcium indicator protein to monitor neuronal activity

    Depositor

    Samuel Wang

    Article

    Sun et al Nat Commun. 2013 Jul 17;4:2170. doi: 10.

    Insert

    Fast-GCaMP-EF16
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    D431N/D433N/D435N/E442A mutated from GCaMP3

    Promoter

    T7

    Available since

    April 23, 2014

    Availability

    Academic Institutions and Nonprofits only

11. Fast-GCaMP-EF19

    Plasmid

    #52644

    Purpose

    genetically encodable fluorescent calcium indicator protein to monitor neuronal activity

    Depositor

    Samuel Wang

    Article

    Sun et al Nat Commun. 2013 Jul 17;4:2170. doi: 10.

    Insert

    Fast-GCaMP-EF19
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    T328D/T330D mutated from GCaMP3

    Promoter

    T7

    Available since

    April 23, 2014

    Availability

    Academic Institutions and Nonprofits only

12. pJBL717

    Plasmid

    #140387

    Purpose

    Expresses MobR protein under the control of T7-lacO promoter

    Depositor

    Julius Lucks

    Article

    Jung et al Nat Biotechnol. 2020 Jul 6. pii: 10.1038

    Insert

    T7-lacO-MobR-T
    

    Use

    Tags

    TEV-6XHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pStrepIHFb

    Plasmid

    #149385

    Purpose

    Expression of P. aeruginosa PA14, StrepII-HRV3C-IHFbeta

    Depositor

    Blake Wiedenheft

    Article

    Santiago-Frangos et al Curr Biol. 2021 Aug 23;31(16):3515-3524.

    Insert

    Pseudomonas aeruginosa PA14 StrepII-tagged IHFbeta
    

    Use

    Tags

    HRV3C cleavage site and StrepII-tag with

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. Human USP30 (64-502, construct 13, MG-26-82)

    Plasmid

    #110746

    Purpose

    Bacterial expression of human USP30 (construct 13)

    Depositor

    David Komander

    Article

    Gersch et al Nat Struct Mol Biol. 2017 Sep 25. doi: 1

    Insert

    USP30 (USP30 Human)
    

    Use

    Tags

    N-His6-3C-GST-3C

    Expression

    Bacterial

    Mutation

    F348D, M350S, I353E + insertion deletion: 64-178;…

    Promoter

    T7

    Available since

    June 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. SENP2M497A

    Plasmid

    #61088

    Purpose

    cleave the SUMO off when use PETSUMO vector for express fusion protein

    Depositor

    Cynthia Wolberger

    Article

    Wiener et al Nat Struct Mol Biol. 2013 Sep;20(9):1033

    Insert

    SENP2M497A catalytic domain (SENP2 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    M497A; Catalytic domain is residues 364-589

    Promoter

    T7

    Available since

    Jan. 29, 2015

    Availability

    Academic Institutions and Nonprofits only

16. pET-28a-GFP-Patronin CC 868–1457

    Plasmid

    #59053

    Purpose

    for E. coli expression of Patronin CC truncation

    Depositor

    Ron Vale

    Article

    Hendershott et al Proc Natl Acad Sci U S A. 2014 Apr 22;11

    Insert

    Patronin (Patronin Fly)
    

    Use

    Tags

    His, Strep, TEV, and sfGFP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

17. pET-28a(+)-His(6)-TEV-AtPCO4-1(wt)

    Plasmid

    #191801

    Purpose

    Full length wt AtPCO4 with Nt-His tag

    Depositor

    Emily Flashman

    Article

    White et al J Biol Chem. 2018 Jul 27;293(30):11786-1

    Insert

    AtPCO4-1
    

    Use

    Tags

    6xHis, TEV cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. HOXC13(human)-pET

    Plasmid

    #8679

    Depositor

    Corey Largman

    Article

    Komuves et al Dev Dyn 2003 Jun;227(2):192-202.

    Insert

    HOXC13 (HOXC13 Human)
    

    Use

    Tags

    His and T7

    Expression

    Bacterial

    Mutation

    makes protein by Coomassie blue staining

    Promoter

    Available since

    May 30, 2006

    Availability

    Academic Institutions and Nonprofits only

19. Pediculus humanus corporis PINK1 (143 - 575)

    Plasmid

    #110751

    Purpose

    Bacterial expression of PhPINK1 (crystallography)

    Depositor

    David Komander

    Article

    Schubert et al Nature. 2017 Dec 7;552(7683):51-56. doi:

    Insert

    Phum_PHUM577390 (Phum_PHUM577390 Pediculus humanus corporis)
    

    Use

    Tags

    N-His6-SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. HOXA9 (murine) HIS-tag pET

    Plasmid

    #8492

    Depositor

    Corey Largman

    Article

    Shen et al Mol Cell Biol 1997 Nov;17(11):6448-58.

    Insert

    HoxA9 (Hoxa9 Mouse)
    

    Use

    Tags

    His and T7

    Expression

    Bacterial

    Mutation

    BamHI/BalI fusion into amino acid #2 of HOXA9; ma…

    Promoter

    Available since

    May 30, 2006

    Availability

    Academic Institutions and Nonprofits only