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pSL7 - Brr2 del247 N1104L

Plasmid

#90257

Purpose

protein expression

Depositor

Christine Guthrie

Article

Ledoux et al J Biol Chem. 2016 Jun 3;291(23):11954-65

Insert

BRR2

Tags

TAP

Expression

Yeast

Mutation

deleted first 247 amino acids and N1104L

Availability

Academic Institutions and Nonprofits only
pSL11 - Brr2 del422 N1104L

Plasmid

#90261

Purpose

protein expression

Depositor

Christine Guthrie

Article

Ledoux et al J Biol Chem. 2016 Jun 3;291(23):11954-65

Insert

BRR2

Tags

TAP

Expression

Yeast

Mutation

deleted first 422 amino acids and amino acid N110…

Availability

Academic Institutions and Nonprofits only

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pFH2.104_PB-EPN24-T2A-eGFP-PGK-Blast

Plasmid

#205549

Purpose

Express EPN24-T2A-eGFP and blasticidin resistance marker

Depositor

Michael Elowitz

Article

Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

Insert

EPN24

Use

Piggybac

Expression

Mammalian

Mutation

WT

Promoter

EF1

Available Since

Sept. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pDF0582 huDisCas7-11 R1045-GGGS-R1122 U6-NT guide

Plasmid

#186991

Purpose

AAV transgene plasmid for Cas7-11-R1045-GGGS-R1122, with U6 promoter-driven expression of non-targeting crRNA guide

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Kato et al Cell. 2022 May 25. pii: S0092-8674(22)00

Insert

cas7-11-r1045-gggs-r1122, non-targeting crRNA guide

Use

AAV

Mutation

r1045-gggs-r1122 deletion

Available Since

Sept. 6, 2022

Availability

Academic Institutions and Nonprofits only

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pBabe puro-HA-PIK3CA(H1047R;F486S;del)

Plasmid

#33255

Depositor

Thomas Roberts

Article

Ilic et al Proc Natl Acad Sci U S A. 2011 Sep 13;10

Insert

PIK3CA (PIK3CA Human)

Use

Retroviral

Tags

Hemagglutinin (HA)

Expression

Mammalian

Mutation

changed histidine 1047 to arginine and phenylalan…

Promoter

SV40

Available Since

Jan. 4, 2012

Availability

Academic Institutions and Nonprofits only
pTS104 pHAGE2 CMVtetO2 MCS-3C-22xGS-GFP

Plasmid

#199357

Purpose

Lentiviral transfer plasmid for doxycycline inducible expression of a C-terminally 3C-GFP-tagged protein in mammalian cells.

Depositor

Rebecca Voorhees

Article

Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

Type

Empty backbone

Use

Lentiviral

Tags

3C-EGFP

Expression

Mammalian

Mutation

WT

Available Since

April 21, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+) ZKSCAN1 MCS-ZKSCAN1 548-1047

Plasmid

#69903

Purpose

Expresses a miniature version of the ZKSCAN1 circular RNA in mammalian cells

Depositor

Jeremy Wilusz

Article

Kramer et al Genes Dev. 2015 Oct 8.

Insert

ZKSCAN1 (ZKSCAN1 Human)

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 21, 2015

Availability

Academic Institutions and Nonprofits only

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pDF0385 pcmv-hudiscas7-11-r1045-gggs-r1122

Plasmid

#186982

Purpose

mammalian expression of cas7-11-r1045-gggs-r1122

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Kato et al Cell. 2022 May 25. pii: S0092-8674(22)00

Insert

cas7-11-r1045-gggs-r1122

Expression

Mammalian

Mutation

r1045-gggs-r1122 deletion

Available Since

Aug. 19, 2022

Availability

Academic Institutions and Nonprofits only

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nCas9-UGI control for Target-AID (pRZ1047)

Plasmid

#131299

Purpose

CAG promoter expression plasmid for NLS-hSpCas9n(D10A)-NLS-SH3-3xFLAG-NLS-UGI-P2A-EGFP (Target-AID without pmCDA1).

Depositor

Keith Joung

Article

Grunewald et al Nat Biotechnol. 2019 Sep 2. pii: 10.1038

Insert

NLS-hSpCas9n(D10A)-NLS-SH3-3xFLAG-NLS-UGI-P2A-EGFP

Expression

Mammalian

Promoter

CAG

Available Since

Oct. 24, 2019

Availability

Academic Institutions and Nonprofits only

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3604 pGEX4T-1 Bad S136A (104-141)

Plasmid

#8801

Depositor

Stanley Korsmeyer

Article

Harada et al Mol Cell 1999 Apr;3(4):413-22.

Insert

Bad (Bad Mouse)

Tags

GST

Expression

Bacterial

Mutation

Contains AA's 104-141; S136A

Promoter

Ptac

Available Since

July 6, 2005

Availability

Academic Institutions and Nonprofits only
pcDNA3.1(+) Laccase2 MCS-ZKSCAN1 548-1047

Plasmid

#69896

Purpose

Expresses a miniature version of the ZKSCAN1 circular RNA in mammalian cells

Depositor

Jeremy Wilusz

Article

Kramer et al Genes Dev. 2015 Oct 8.

Insert

ZKSCAN1 (stw, ZKSCAN1 Human)

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 21, 2015

Availability

Academic Institutions and Nonprofits only

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Hy_pMT Laccase2 MCS-ZKSCAN1 548-1047

Plasmid

#69886

Purpose

Expresses a miniature version of the ZKSCAN1 circular RNA in Drosophila

Depositor

Jeremy Wilusz

Article

Kramer et al Genes Dev. 2015 Oct 8.

Insert

ZKSCAN1 (stw, ZKSCAN1 Human)

Expression

Insect

Promoter

Metallothionein Promoter (pMT)

Available Since

Nov. 11, 2015

Availability

Academic Institutions and Nonprofits only

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3603 pGEX4T-1 Bad S112A (104-141)

Plasmid

#8802

Depositor

Stanley Korsmeyer

Article

Harada et al Mol Cell 1999 Apr;3(4):413-22.

Insert

Bad (Bad Mouse)

Tags

GST

Expression

Bacterial

Mutation

Contains AA's 104-141; S112A

Promoter

Ptac

Available Since

July 6, 2005

Availability

Academic Institutions and Nonprofits only
pET-28b His6-TEV-SHP2-FL(C104P)

Plasmid

#245202

Purpose

Production of His-tagged full-length SHP2

Depositor

Neel Shah

Article

Jiang et al Nat Commun. 2025 Jul 1;16(1):5464. doi:

Insert

SHP2 full-length

Tags

6xHis

Expression

Bacterial

Mutation

C104P

Available Since

Oct. 2, 2025

Availability

Academic Institutions and Nonprofits only

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HCT12_rAAV-eHGT_1049m-minBglobin-SYFP2-WPRE3-BGHpA

Plasmid

#223910

Purpose

Direct-expressing SYFP2 AAV virus

Depositor

Tanya Daigle, Allen Institute for Brain Science

Article

Kussick et al Cell Rep. 2025 Jun 24;44(6):115730. doi:

Insert

SYFP2

Use

AAV

Mutation

N/A

Promoter

minBglobin

Available Since

June 27, 2025

Availability

Academic Institutions and Nonprofits only

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pLIB-FLAG-GST(N)-CDK12RS(104-380)

Plasmid

#231738

Purpose

Protein expression in insect cells

Depositor

Jesper Svejstrup

Article

Lopez Martinez et al Mol Cell. 2025 May 15;85(10):1952-1967.e

Insert

CDK12 (CDK12 Human)

Tags

Flag-GST-3C

Expression

Insect

Mutation

CDK12(104-380)

Available Since

May 2, 2025

Availability

Academic Institutions and Nonprofits only

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pLVX-shRNA2-zsGreen-PGK-puro_HSALNG0104472-163

Plasmid

#197902

Purpose

For the stable knockdown experiments using shRNA, a complementary sequence (HSALNG0104472-163) targeting the HSALNG0104472 transcript was inserted.

Depositor

Bo Wang

Article

Lu et al Commun Biol. 2023 Feb 17;6(1):189. doi:

Insert

HSALNG0104472-163 (HSALNG0104472-scramble-sequence)

Use

Lentiviral

Tags

zsGreen1

Expression

Mammalian

Mutation

15q11.2 deletion

Available Since

Nov. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmPAN2-del1047-1063GSG-dsRNAres_Y

Plasmid

#148040

Purpose

Insect Expression of DmPAN2-del1047-1063GSG-dsRNAres

Depositor

Elisa Izaurralde

Article

Jonas et al Nat Struct Mol Biol. 2014 Jul;21(7):599-

Insert

DmPAN2-del1047-1063GSG-dsRNAres (PAN2 Fly)

Expression

Insect

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pTS1047-Tier1-PhCMV-YPet-p2A-PuroR

Plasmid

#169498

Purpose

Tier-1 vector encoding PhCMV-driven YPet in combination with a marker for puromycin selection (PhCMV-YPet-p2A-PuroR-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PCMV-driven YPet-P2aA-PuroR

Expression

Mammalian

Promoter

PhCMV

Available Since

June 14, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3HA-AMOT p130∆PPxY1(E104K/E105K)

Plasmid

#171130

Purpose

expresses AMOT(E104K/E105K) in mammalian cells

Depositor

Wesley Sundquist

Article

Rheinemann et al J Biol Chem. 2021 Jul 17:100975. doi: 10

Insert

Angiomotin p130 E104K/E105K (AMOT Human)

Tags

2xHA

Expression

Mammalian

Mutation

changed glutamic acid residues 104 and 105 to lys…

Available Since

July 23, 2021

Availability

Academic Institutions and Nonprofits only

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