We narrowed to 4,415 results for: Ado;

1. pcDNA3.3_CoV1_D28

   Plasmid

   #170447

   Purpose

   Express SARS-CoV-1 WT spike protein with a 28aa deletion on the C-terminal tail. Used for SARS1 pseudotyped virus production.

   Depositor

   David Nemazee

   Article

   Rogers et al Science. 2020 Aug 21;369(6506):956-963.

   Insert

   SARS-CoV-1 spike D28 (S SARS-CoV-1)
   

   Use

   Tags

   NA

   Expression

   Mammalian

   Mutation

   last 28aa deletion in c-terminal tail

   Promoter

   CMV

   Available since

   May 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCDNA3.3_MERS_D12

   Plasmid

   #170448

   Purpose

   Express MERS WT spike protein with an 12aa deletion on the C-terminal tail. Used for MERS pseudotyped virus production.

   Depositor

   David Nemazee

   Article

   Rogers et al Science. 2020 Aug 21;369(6506):956-963.

   Insert

   MERS spike D12 (S MERS)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   last 12aa deletion in c-terminal tail

   Promoter

   CMV

   Available since

   July 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. PGKp-GFP-YAP

   Plasmid

   #174172

   Purpose

   Lentiviral vector to constitutively express YAP under control of the human PGK promoter.

   Depositor

   Rod Bremner

   Article

   Pearson et al Cancer Cell. 2021 Jul 13. pii: S1535-610

   Insert

   YAP (YAP1 Human)
   

   Use

   Lentiviral

   Tags

   3x FLAG tag

   Expression

   Mammalian

   Mutation

   Promoter

   PGK

   Available since

   Aug. 19, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. EFSp-GFP-YAP (S94A)

   Plasmid

   #174169

   Purpose

   Lentiviral vector to constitutively express TEAD-binding mutant YAP under control of the EFS promoter.

   Depositor

   Rod Bremner

   Article

   Pearson et al Cancer Cell. 2021 Jul 13. pii: S1535-610

   Insert

   YAP (YAP1 Human)
   

   Use

   Lentiviral

   Tags

   3x FLAG tag

   Expression

   Mammalian

   Mutation

   Serine 94 mutated to Alanine

   Promoter

   EFS

   Available since

   Aug. 19, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. PGKp-GFP-TAZ

   Plasmid

   #174176

   Purpose

   Lentiviral vector to constitutively express TAZ/WWTR1 under control of the human PGK promoter.

   Depositor

   Rod Bremner

   Article

   Pearson et al Cancer Cell. 2021 Jul 13. pii: S1535-610

   Insert

   TAZ (WWTR1 Human)
   

   Use

   Lentiviral

   Tags

   Myc tag

   Expression

   Mammalian

   Mutation

   Promoter

   PGK

   Available since

   Aug. 19, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. pLKO-Tet-On-YAP1-shRNA1

   Plasmid

   #176846

   Purpose

   lentiviral vector for doxycycline-inducible human YAP1 knockdown

   Depositor

   Roland Friedel

   Article

   Junqueira Alves et al Nat Commun. 2021 Oct 14;12(1):6019. doi:

   Insert

   YAP1-Sh1 (YAP1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLKO-Tet-On-YAP1-shRNA2

   Plasmid

   #176847

   Purpose

   lentiviral vector for doxycycline-inducible human YAP1 knockdown

   Depositor

   Roland Friedel

   Article

   Junqueira Alves et al Nat Commun. 2021 Oct 14;12(1):6019. doi:

   Insert

   YAP1-Sh2 (YAP1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET28 His-MBP-TEV-BAP-Human BCKDK

   Plasmid

   #232116

   Purpose

   For bacterial expression of Human BCKDK (AA31–412, missing precursor peptide) with an N-terminal biotin acceptor peptide and a cleavable His-MBP purification tag.

   Depositor

   Ruth Sommese

   Article

   Liu et al J Biol Chem. 2023 Mar;299(3):102959. doi

   Insert

   BCKDK (BCKDK Human)
   

   Use

   Tags

   6 x His, Biotin acceptor peptide (BAP), Maltose-B…

   Expression

   Bacterial

   Mutation

   Promoter

   T7 Promoter

   Available since

   March 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA5 frt/to N-BioTAP-C-BRD4-NUT

   Plasmid

   #171630

   Purpose

   Expresses BioTAP-tagged BRD4-NUT in mammalian cells. Can be inserted into a FRT site by co-transfection with a plasmid encoding Flp recombinaase.

   Depositor

   Chris French, Christopher A French

   Article

   Alekseyenko et al Proc Natl Acad Sci U S A. 2017 May 23;11

   Insert

   BRD4-NUT (BRD4, NUTM1 Human)
   

   Use

   Tags

   BioTAP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCRISPaint-mNeon-F-mgp100-PuroR [M1G]

    Plasmid

    #171802

    Purpose

    Universal donor plasmid for CRISPitope method encoding mNeon fluorescent protein, FLAG tag, murine gp100 CD8+ T cell epitope [AA: 25-33 (EGSRNQDWL)] and puromycin resistance cassette [p.M1G]

    Depositor

    Michael Hoelzel

    Article

    Effern et al Immunity. 2020 Sep 15;53(3):564-580.e9.

    Insert

    mNeonGreen-FLAG-mgp100-T2A-PuroR
    

    Use

    Gene tagging

    Tags

    Expression

    Mutation

    PuroR: Changed Methionin 1 to Glycine.

    Promoter

    Available since

    July 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCAG-1BPNLS-Cas9-1BPNLS-2AGFP

    Plasmid

    #87109

    Purpose

    BPNLS-Cas9-BPNLS-2AGFP expression plasmid

    Depositor

    Juan Belmonte

    Article

    Suzuki et al Nature. 2016 Dec 1;540(7631):144-149. do

    Insert

    1BPNLS-Cas9-1BPNLS-2A-EGFP
    

    Use

    CRISPR

    Tags

    3xFLAG (on Cas9)

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    March 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLEX-EF1a ANXA11-mEmerald D40G

    Plasmid

    #164211

    Purpose

    pLEX lentivirus backbone expresses mEmerald tagged ANXA11 D40G mutant under EF1a promoter

    Depositor

    Jennifer Lippincott-Schwartz

    Article

    Liao et al Cell. 2019 Sep 19;179(1):147-164.e20. do

    Insert

    ANXA11 (ANXA11 Human)
    

    Use

    Lentiviral

    Tags

    mEmerald

    Expression

    Mutation

    D40G

    Promoter

    EF1a

    Available since

    Jan. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. CMV-CRY2oligo-mCherry-ANXA11(186-505 aa)

    Plasmid

    #164208

    Purpose

    Expresses CRY2oligo-mcherry tagged ANXA11 C terminal under CMV promoter

    Depositor

    Jennifer Lippincott-Schwartz

    Article

    Liao et al Cell. 2019 Sep 19;179(1):147-164.e20. do

    Insert

    ANXA11 (ANXA11 Human)
    

    Use

    Tags

    CRY2oligo-mcherry

    Expression

    Mammalian

    Mutation

    186-505 aa

    Promoter

    CMV

    Available since

    Jan. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. CMV-CRY2oligo-mcherry-ANXA11(1-185aa)

    Plasmid

    #164207

    Purpose

    Expresses CRY2oligo-mcherry tagged ANXA11 N terminal under CMV promoter

    Depositor

    Jennifer Lippincott-Schwartz

    Article

    Liao et al Cell. 2019 Sep 19;179(1):147-164.e20. do

    Insert

    ANXA11 (ANXA11 Human)
    

    Use

    Tags

    CRY2oligo-mcherry

    Expression

    Mammalian

    Mutation

    1-185 aa

    Promoter

    CMV

    Available since

    Jan. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pPHR-mCherry-mHP1alpha

    Plasmid

    #181883

    Purpose

    Soluble light-dependent effector protein (mouse HP1alpha) for optodroplet formation or recruitment to CIBN localizers

    Depositor

    Karsten Rippe

    Article

    Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.

    Insert

    PHR-mCherry-mHP1alpha (Cbx5 Mustard Weed, Mouse)
    

    Use

    Tags

    PHR-mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. JDW 742 (pDONR221-EGFP-KRAS4B-G12V)

    Plasmid

    #156403

    Purpose

    Gateway middle entry clone encoding EGFP-fused to human KRAS4B with G12V mutation, includes a stop codon

    Depositor

    Joshua Wythe

    Article

    Fish et al Circ Res. 2020 Jun 17. doi: 10.1161/CIRC

    Insert

    human mutant KRAS4B-G12V (KRAS Human)
    

    Use

    Gateway cloning

    Tags

    EGFP

    Expression

    Mutation

    mutant KRAS4B-G12V

    Promoter

    Available since

    Aug. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pPHR-mCherry-hNPM

    Plasmid

    #181887

    Purpose

    Soluble light-dependent effector protein (nucleophosmin) for optodroplet formation or recruitment to CIBN localizers

    Depositor

    Karsten Rippe

    Article

    Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.

    Insert

    PHR-mCherry-hNPM (NPM1 Human, Mustard Weed)
    

    Use

    Tags

    PHR-mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pPHR-mCherry-hHP1gamma

    Plasmid

    #181885

    Purpose

    Soluble light-dependent effector protein (human HP1gamma) for optodroplet formation or recruitment to CIBN localizers

    Depositor

    Karsten Rippe

    Article

    Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.

    Insert

    PHR-mCherry-hHP1gamma (CBX3 Human, Mustard Weed)
    

    Use

    Tags

    PHR-mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pENTR D-TOPO-C3_33

    Plasmid

    #60275

    Purpose

    This plasmid contains a human pancreatic islet active enhancer, which can be shuttled into a Gateway destination vector, such as pGL4.23-Gateway.

    Depositor

    Jorge Ferrer

    Article

    Pasquali et al Nat Genet. 2014 Feb;46(2):136-43. doi: 1

    Insert

    GLIS3 enhancer (GLIS3 Human)
    

    Use

    Entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. JDW 837 (pCS2-V5-mScarlet-I-hsKRAS4A-G12V)

    Plasmid

    #156410

    Purpose

    pCS2 CMV expression vector driving V5-tagged mScarlet-I-fused human KRAS4A-G12V

    Depositor

    Joshua Wythe

    Article

    Fish et al Circ Res. 2020 Jun 17. doi: 10.1161/CIRC

    Insert

    human mutant KRAS4A-G12V (KRAS Human)
    

    Use

    Tags

    mScarlet-I

    Expression

    Mammalian

    Mutation

    mutant KRAS4A-G12V

    Promoter

    CMV

    Available since

    Aug. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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