We narrowed to 7,590 results for: Lif;

1. EGFP-uTEV1-MAVS

   Plasmid

   #170998

   Purpose

   HiLITR protease with MAVS c-terminal tmd targeting information (Mitochondria)

   Depositor

   Alice Ting

   Article

   Coukos et al eLife. 2021 Aug 20;10. pii: 69142. doi:

   Insert

   EGFP-uTEV1-MAVS(tmd)
   

   Use

   Lentiviral and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   pTRE-tight (TetOn)

   Available since

   Aug. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET151-Vps4(101-437)-Hcp1

   Plasmid

   #87737

   Purpose

   Bacterial expression of S. cerevisiae Vps4 AAA ATPase cassette fused to P. aeruginosa Hcp1

   Depositor

   Chris Hill

   Article

   Monroe et al Elife. 2017 Apr 5;6. pii: e24487. doi: 1

   Insert

   Vacuolar protein sorting-associated protein 4 (VPS4 Budding Yeast)
   

   Use

   Tags

   6xHis-V5-TEV and Hcp1

   Expression

   Bacterial

   Mutation

   deleted amino acids 1-100

   Promoter

   T7

   Available since

   May 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCDNA3 V5 mDispatched WT 2x HA

   Plasmid

   #126410

   Purpose

   Expression of V5 Dispatched WT HA in mammalian cell lines.

   Depositor

   Stacey Ogden

   Article

   Stewart et al Elife. 2018 Jan 23;7. pii: 31678. doi: 1

   Insert

   m-Dispatched1 (Disp1 Mouse)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   V5 iserted between A106 and A107

   Promoter

   Available since

   June 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-DIO-CaMKII-TVA-P2A-N2cG

   Plasmid

   #172362

   Purpose

   Targeting envA-pseodotyped RVdG-CVS-N2c vectors for specific retrograde labeling from cre-expressing excitatory cortical neurons

   Depositor

   Yoav Ben-Simon, Peter Jonas

   Article

   Sumser et al Elife. 2022 Aug 30;11:e79848. doi: 10.75

   Insert

   TVA + B19-N2cG
   

   Use

   AAV and Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CaMKIIa

   Available since

   Sept. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCA528 SPASTIN , residues 108-200

   Plasmid

   #180628

   Purpose

   Bacterial expression for SPASTIN MIT, residues 108-200. Has N-term HIS-SUMO tag. Used for FP experiments. Internal ID: WISP21-10.

   Depositor

   Wesley Sundquist

   Article

   Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

   Insert

   SPASTIN
   

   Use

   Tags

   HIS-SUMO

   Expression

   Bacterial

   Mutation

   Residues 108-200

   Promoter

   T7

   Available since

   July 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDM1383

   Plasmid

   #207423

   Purpose

   Extrachromosomal expression HSPC300-GFP and mCherry-Hs Raf1 (1–134 RBD)

   Depositor

   Rob Kay

   Article

   Veltman et al Elife. 2016 Dec 13;5:e20085. doi: 10.755

   Inserts

   HSPC300
   Raf1 (RAF1 Human)
   

   Use

   Unspecified

   Tags

   GFP and mCherry

   Expression

   Mutation

   aa 1–134 (RBD) only

   Promoter

   Available since

   Nov. 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.1-ROR1-V5/HIS

   Plasmid

   #205607

   Purpose

   Expression of human ROR1 receptor tyrosine kinase in mammalian cells

   Depositor

   Pavel Krejčí

   Article

   Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

   Insert

   ROR1 receptor tyrosine kinase (ROR1 Human)
   

   Use

   Tags

   V5-His

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.1-SARS2-Spike-D614G

   Plasmid

   #166850

   Purpose

   For expression of SARS-CoV-2 Spike D614G in mammalian cells (C9 tagged at C-term)

   Depositor

   Neville Sanjana

   Article

   Daniloski et al Elife. 2021 Feb 11;10. pii: 65365. doi:

   Insert

   SARS-CoV-2 Spike (S SARS-CoV-2 virus)
   

   Use

   Tags

   C9

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 7, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. Sema3e(L)-Fc-His

   Plasmid

   #72145

   Purpose

   Expresses the Sema3E protein (truncated at cleavage site P3; ie, long), C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

   Depositor

   Woj Wojtowicz

   Article

   Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

   Insert

   Sema3e (Sema3e Mouse)
   

   Use

   Tags

   Fc-His

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 26, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA pDEST40 Cas9-HMGB1

    Plasmid

    #183195

    Purpose

    Expression clone

    Depositor

    Emma Haapaniemi

    Article

    Reint et al Elife. 2021 Dec 13;10. pii: 75415. doi:

    Insert

    HMGB1
    

    Use

    CRISPR

    Tags

    Cas9WT

    Expression

    Mammalian

    Mutation

    n/a

    Promoter

    Available since

    Sept. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. CVS-N2c-nl.EGFP-jRGECO1a

    Plasmid

    #172386

    Purpose

    Production of RVdG-CVS-N2c viral vectors for cell-type specific trans-synaptic retrograde labeling.

    Depositor

    Yoav Ben-Simon, Peter Jonas

    Article

    Sumser et al Elife. 2022 Aug 30;11:e79848. doi: 10.75

    Insert

    nuclear-localized EGFP + jRGECO1a
    

    Use

    Neurotropic virus

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTol1-UAS:ChR2(H134R)-tdTomato

    Plasmid

    #124237

    Purpose

    Zebrafish Tol1 construct for UAS-mediated expression of the optogenetic opsin ChR2(H134R) fused to the red fluorescent protein tdTomato

    Depositor

    Isaac Bianco

    Article

    Antinucci et al Elife. 2020 Mar 27;9. pii: 54937. doi: 1

    Insert

    ChR2(H134R)-tdTomato
    

    Use

    Zebrafish expression

    Tags

    tdTomato

    Expression

    Mutation

    Promoter

    14xUAS-E1b

    Available since

    Jan. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. plKO.1-DNAJC5-ShRNA

    Plasmid

    #205729

    Purpose

    Knockdown of DNAJC5

    Depositor

    Randy Schekman

    Article

    Wu et al Elife. 2023 Jan 10;12:e85837. doi: 10.75

    Insert

    shRNA targeting DNAJC5 (DNAJC5 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCA528 SPASTIN , residues 112-196

    Plasmid

    #180627

    Purpose

    Bacterial expression for SPASTIN MIT, residues 112-196. Has N-term HIS-SUMO tag. Used for X-ray crystallography. Internal ID:WISP21-111.

    Depositor

    Wesley Sundquist

    Article

    Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

    Insert

    SPASTIN
    

    Use

    Tags

    HIS-SUMO

    Expression

    Bacterial

    Mutation

    Residues 112-196

    Promoter

    T7

    Available since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. Sema6d.1-AP-His

    Plasmid

    #72042

    Purpose

    Expresses the extracellular region of the Sema6D, isoform 1 protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

    Depositor

    Woj Wojtowicz

    Article

    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

    Insert

    Sema6d.1 (Sema6d Mouse)
    

    Use

    Tags

    AP-His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. All-in-one split ADAR2(E488Q)

    Plasmid

    #170137

    Purpose

    AAV vector carrying ADAR2-DDC(E488Q)-λN-P2A-MCP-ADAR2-DDN with a nuclear export signal

    Depositor

    Prashant Mali

    Article

    Katrekar et al Elife. 2022 Jan 19;11. pii: 75555. doi:

    Insert

    ADAR2-DDC(E488Q)-λN-P2A-MCP-ADAR2-DDN
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. Tom70NTD-ABHD16Acyto-mNG

    Plasmid

    #195159

    Purpose

    mammalian expression of outer mitochondrial membrane localized ABHD16A tagged with mNeonGreen

    Depositor

    Gia Voeltz

    Article

    Nguyen et al Elife. 2022 Nov 30;11:e84279. doi: 10.75

    Insert

    Tom70NTD (TOMM70 Human)
    

    Use

    Tags

    ABHD16A cytoplasmic domain and mNeonGreen

    Expression

    Mammalian

    Mutation

    None

    Promoter

    CMV

    Available since

    Feb. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. Sema4a-Fc-His

    Plasmid

    #72154

    Purpose

    Expresses the extracellular region of the Sema4A protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

    Depositor

    Woj Wojtowicz

    Article

    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

    Insert

    Sema4a (Sema4a Mouse)
    

    Use

    Tags

    Fc-His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAAV-β2AR-NNES-NanoLuc-eLOV-TEVcs-Flag-Gal4-V5

    Plasmid

    #125236

    Purpose

    Expresses modified SPARK β2AR-NanoLuc transmembrane component in mammalian cells

    Depositor

    Alice Ting

    Article

    Kim et al eLife. 2019 Apr 3;8. pii: 43826. doi: 10

    Insert

    β2AR-NNES-NanoLuc-eLOV-TEVcs-Flag-Gal4-V5
    

    Use

    AAV

    Tags

    Flag, V5

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCMV-DNAJC5 (WT)-HaloTag

    Plasmid

    #205710

    Purpose

    Mammalian expression of DNAJC5 (WT)-HaloTag

    Depositor

    Randy Schekman

    Article

    Wu et al Elife. 2023 Jan 10;12:e85837. doi: 10.75

    Insert

    DNAJC5 (DNAJC5 Human)
    

    Use

    Tags

    HaloTag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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