We narrowed to 2,321 results for: pcas9

1. CRISPR-SB

   Plasmid

   #177936

   Purpose

   Expresses SpCas9 and sgRNA. Can be integrated into the genome upon co-delivery of Sleeping Beauty transposase.

   Depositor

   Roland Rad

   Article

   Kaltenbacher et al Nat Protoc. 2022 Apr;17(4):1142-1188. do

   Type

   Empty backbone

   Use

   CRISPR; Transposon

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Nov. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pT7-PE7 for IVT

   Plasmid

   #214813

   Purpose

   Template for in vitro transcription of PE7

   Depositor

   Brittany Adamson

   Article

   Yan et al Nature. 2024 Apr 3. doi: 10.1038/s41586-

   Insert

   PE7
   

   Use

   Template for in vitro transcription

   Tags

   SV40 bpNLS and c-Myc NLS

   Expression

   Mutation

   Promoter

   T7 (inactivated)

   Available since

   March 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. Polymerase expression - pCMV-T7-EcKlenow (LM2692)

   Plasmid

   #208963

   Purpose

   Unfused EcKlenow DNA polymerase (-exo), expressed from CMV or T7 promoters.

   Depositor

   Benjamin Kleinstiver

   Article

   Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

   Insert

   EcKlenow-BPNLS
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS

   Expression

   Mammalian

   Mutation

   EcKlenow(-exo;D355A/E357A)

   Promoter

   CMV and T7

   Available since

   Nov. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. EF1a-TET1-dCas9

   Plasmid

   #235593

   Purpose

   Tet1CD fused to N-terminus of dCas9

   Depositor

   Ting Zhou

   Article

   Wu et al Stem Cell Reports. 2025 Apr 25:102499. d

   Insert

   TET1-dCas9 (TET1 SpCas9 is from Streptococcus pyogenes, Human)
   

   Use

   CRISPR

   Tags

   3xNLS, TET1 CD, and tagBFP

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   May 8, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pX330-sgR26

   Plasmid

   #127376

   Purpose

   Used for contransfection with pR26- and pR26-CMVconst-derived constructs to mediate CRISPR/Cas9 genomic insertion into the murine ROSA26 safe harbor locus.

   Depositor

   Lance Miller

   Article

   Routh et al Front Immunol. 2020 Feb 13;11:57. doi: 1

   Insert

   ROSA26 (Gt(ROSA)26Sor Mouse)
   

   Use

   CRISPR and Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pX330-PITCh-LMNB1

   Plasmid

   #207770

   Purpose

   Expresses SpCas9, the PITCh gRNA, and a sgRNA targeting the N-terminus of LMNB1 for knock-in.

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   sgRNA Targeting N-terminus of LMNB1 (LMNB1 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Nov. 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pX330-PITCh-ACTB

   Plasmid

   #207748

   Purpose

   Expresses SpCas9, the PITCh gRNA, and a sgRNA targeting the N-terminus of ACTB for knock-in.

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   sgRNA Targeting N-terminus of ACTB (ACTB Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Nov. 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. p2T-U6-sgGAA

   Plasmid

   #232724

   Purpose

   Cas9 guide RNA targeting GAA repeats for A-to-G base editing

   Depositor

   David Liu

   Article

   Matuszek et al Nat Genet. 2025 May 26. doi: 10.1038/s41

   Insert

   SpCas9 gRNA targeting GAA repeats
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pABE8e-NG-403Q-Nterm-AAV

   Plasmid

   #194651

   Purpose

   N-terminal AAV genome plasmid encoding ABE8e + sgRNA to correct the R403Q mutation in mice

   Depositor

   David Liu

   Article

   Reichart et al Nat Med. 2023 Feb;29(2):412-421. doi: 10

   Insert

   ABE8e-NG N-term
   

   Use

   AAV

   Tags

   BPNLS and NpuN spilt intein

   Expression

   Mutation

   Promoter

   TNNT2

   Available since

   Feb. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pABE8e-NG-403Q-Cterm-AAV

    Plasmid

    #194652

    Purpose

    C-terminal AAV genome plasmid encoding ABE8e + sgRNA to correct the R403Q mutation in mice

    Depositor

    David Liu

    Article

    Reichart et al Nat Med. 2023 Feb;29(2):412-421. doi: 10

    Insert

    ABE8e-NG C-term
    

    Use

    AAV

    Tags

    BPNLS and NpuC split intein

    Expression

    Mutation

    Promoter

    TNNT2

    Available since

    Feb. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. SGyA

    Plasmid

    #160292

    Purpose

    Drives expression of Cas9 and a TdTomato fluorescent marker

    Depositor

    Omar Akbari

    Article

    Gamez et al Nat Commun. 2021 Dec 10;12(1):7202. doi:

    Inserts

    Vas promoter
    hSpCas9
    tdTomato
    CTCF insulator
    Gypsy insulator
    Left homology arm Y chromosome
    Right homology arm Y chromosome
    

    Use

    Tags

    T2A GFP

    Expression

    Insect

    Mutation

    Promoter

    Available since

    March 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. TadA-NG-CBE

    Plasmid

    #227420

    Purpose

    C-to-T base editing in mammalian cells

    Depositor

    Yang Sun

    Article

    Liu et al Adv Sci (Weinh). 2024 Sep 19:e2405628. d

    Insert

    TadA-nSpCas9-NG-2xUGI (AICDA Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGES401

    Plasmid

    #190198

    Purpose

    For CRISPR-Cas9 mediated multiple gene editing in soybean, single transcript unit system driven by soybean elongation factor 1A promoter (pM4), Basta selection

    Depositor

    Yuefeng Guan

    Article

    Bai et al Mol Plant. 2022 Jul 4;15(7):1081-1083. d

    Insert

    spCas9
    

    Use

    CRISPR

    Tags

    3xFlag

    Expression

    Plant

    Mutation

    plant-codon optimized

    Promoter

    Glycine max elongation factor 1A(pM4)

    Available since

    Sept. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. ABE7.10-F148A

    Plasmid

    #132946

    Purpose

    Gene editing. See Gene/Insert section of plasmid page for targeting sequence cloned into this plasmid.

    Depositor

    Hui Yang

    Article

    Zhou et al Nature. 2019 Jul;571(7764):275-278. doi:

    Insert

    ABE7.10(F148A)
    

    Use

    CRISPR; Base editor

    Tags

    Expression

    Mammalian

    Mutation

    ABE7.10(F148A)

    Promoter

    Available since

    Dec. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pX330-PITCh-COX8A

    Plasmid

    #227308

    Purpose

    Expresses SpCas9, the PITCh gRNA, and a sgRNA targeting the C-terminus of COX8A for knock-in.

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    sgRNA Targeting C-terminus of COX8A (COX8A Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U67

    Available since

    Nov. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. CGBE1-NG (pBM1246)

    Plasmid

    #140256

    Purpose

    CMV promoter expression plasmid for UNG(E.coli)-rAPOBEC1(R33A)-nCas9_NG-P2A-EGFP

    Depositor

    Keith Joung

    Article

    Kurt et al Nat Biotechnol. 2020 Jul 20. pii: 10.103

    Insert

    eUNG-BE4max(R33A)_NG-ΔUGI
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    R33A in rAPOBEC1, NG mutations in SpCas9, D10A in…

    Promoter

    CMV

    Available since

    Aug. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pX330-PITCh-PEX3

    Plasmid

    #227303

    Purpose

    Expresses SpCas9, the PITCh gRNA, and a sgRNA targeting the C-terminus of PEX3 for knock-in.

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    sgRNA Targeting C-terminus of PEX3 (PEX3 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Nov. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pRS0045

    Plasmid

    #131124

    Purpose

    Mammalian Target-ACE expression vector

    Depositor

    Nozomu Yachie

    Article

    Sakata et al Nat Biotechnol. 2020 Jun 1. pii: 10.1038

    Insert

    TadA-linker(32aa)-TadA*(7.10)-linker(32aa)-nCas9-PmCDA1-UGI
    

    Use

    CRISPR

    Tags

    PmCDA1, TadA, and UGI

    Expression

    Mammalian

    Mutation

    A262T, R324L, S409I, E480K, E543D, M694I, E1219V …

    Promoter

    CMV promoter

    Available since

    Sept. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. Px461-Cas9n-Trp53-sgRNA-beta

    Plasmid

    #88847

    Purpose

    CRISPR KO of Trp53

    Depositor

    Joan Massague

    Article

    Wang et al Cell Stem Cell. 2017 Jan 5;20(1):70-86.

    Insert

    Trp53 (Trp53 Mouse)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 11, 2017

    Availability

    Academic Institutions and Nonprofits only

20. Px461-Cas9n-Trp53-sgRNA-alpha

    Plasmid

    #88846

    Purpose

    CRISPR KO of Trp53

    Depositor

    Joan Massague

    Article

    Wang et al Cell Stem Cell. 2017 Jan 5;20(1):70-86.

    Insert

    Trp53 (Trp53 Mouse)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 11, 2017

    Availability

    Academic Institutions and Nonprofits only