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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 3,270 results for: Streptococcus

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Showing: 2141 - 2160 of 3270 results

  1. pCMV-PE2-tagRFP-BleoR

    Plasmid
    #192508
    Purpose
    Expression of PE2 in mammalian cells with co-expression of tagRFP and Zeocin resistance gene.
    Depositor
    Gerald Schwank
    Publication
    Mathis et al Nat Biotechnol. 2023 Jan 16. doi: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    PE2-P2A-tagRFP-P2A-BleoR
    Tags
    BleoR, SV40 bpNLS, and tagRFP
    Expression
    Mammalian
    Available since
    Jan. 19, 2023
    Availability
    Academic Institutions and Nonprofits only
    192508-plasmid-map-sequence-id-377009

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  2. cr3Ec

    Plasmid
    #184860
    Purpose
    cr3 helper plasmid for recombination and Cas9 induction; harbors the Cas9 endonuclease and an E.coli-specific recombination system
    Depositor
    Patrick Sobetzko
    Publication
    Teufel et al Nat Commun. 2022 Jun 14;13(1):3430. doi:
    Insert
    Cas9, gam, bet, exo
    Tags
    Cas9 ssrA degradation tag
    Expression
    Bacterial
    Available since
    Jan. 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    184860-plasmid-map-sequence-id-363182

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  4. pJEC726

    Plasmid
    #190814
    Purpose
    CRISPRi backbone plasmid harboring BbsI sites for easy cloning of sgRNA targeting regions via Golden gate.
    Depositor
    James Chappell
    Publication
    Ameruoso et al Nucleic Acids Res. 2022 Jul 22;50(13):77
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dCas9
    Use
    CRISPR and Synthetic Biology
    Mutation
    S. pyogenes dCas9 codon optimized for Streptomyce…
    Promoter
    SP30
    Available since
    Sept. 30, 2022
    Availability
    Academic Institutions and Nonprofits only
    190814-plasmid-map-sequence-id-373712

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  10. pAAVS1-CAG-hCAS9-neo

    Plasmid
    #166026
    Purpose
    AAVS1 targeting vector carrying CAG-SpCas9-neo
    Depositor
    Kosuke Yusa
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    AAVS1-CAGCas9IRESneo
    Expression
    Mammalian
    Available since
    March 18, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    166026-plasmid-map-sequence-id-328056

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  12. pCC_04 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-xCas9NG-NLS-2A-Puro-WPRE

    Plasmid
    #139089
    Purpose
    Expresses human codon-optimized xCas9-NG nuclease in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a unique barcode downstream of sgRNA cassette.
    Depositor
    Neville Sanjana
    Publication
    Legut et al Cell Rep. 2020 Mar 3;30(9):2859-2868.e5.
    Insert
    xCas9-NG
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Mutation
    A262T,R324L, S409I, E480K, E543D, M694I, L1111R, …
    Available since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    139089-plasmid-map-sequence-id-273885

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  13. pRS0035

    Plasmid
    #131125
    Purpose
    Mammalian Target-AID expression vector
    Depositor
    Nozomu Yachie
    Publication
    Sakata et al Nat Biotechnol. 2020 Jun 1. pii: 10.1038
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    nCas9-PmCDA1-UGI
    Use
    CRISPR
    Tags
    PmCDA1 and UGI
    Expression
    Mammalian
    Mutation
    D10A in SpCas9
    Promoter
    CMV promoter
    Available since
    Sept. 23, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    131125-plasmid-map-sequence-id-257456

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  14. pBAD33.1-DpnII

    Plasmid
    #127413
    Purpose
    Expresses DpnII restriction endonuclease in bacteria
    Depositor
    Douglas Feldman
    Insert
    DpnII
    Expression
    Bacterial
    Promoter
    pBAD
    Available since
    June 25, 2019
    Availability
    Academic Institutions and Nonprofits only
    127413-plasmid-map-sequence-id-247669

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  16. pAAV-SCP1-dSa VPR mini.-2X snRP-1 Hbb

    Plasmid
    #99692
    Purpose
    Expresses dSa Cas9 fused to optimized combination of truncated activation domains from SCP1 promoter with dual snRP1 poly adenylation signal, and Sa sgRNA for Hbb, vector allows for strong activation of mouse Hbb, can be packaged and delivered as AAV
    Depositor
    George Church
    Publication
    Vora et al bioRxiv 2018. 298620
    Insert
    dCas9 and gRNA targeting Hbb (Hbb Synthetic)
    Use
    AAV
    Tags
    VPR mini
    Mutation
    dead Cas9
    Available since
    Jan. 16, 2019
    Availability
    Academic Institutions and Nonprofits only
    99692-plasmid-map-sequence-id-194594

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Showing: 2141 - 2160 of 3270 results