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We narrowed to 8,368 results for: pcDNA3

Showing: 2141 - 2160 of 8368 results

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pcDNA3.1(+)_Granulin 1+linker2

Plasmid

#176918

Purpose

express Granulin 1 with linker 2 in mammalian cells

Depositor

Thomas Kukar

Article

Holler et al eNeuro. 2017 Aug 18;4(4). pii: eN-NWR-01

Insert

Granulin 1+linker2 (GRN Human)

Use

Tags

Twin-Strep and FLAG tags encoded after Signal Pep…

Expression

Mammalian

Mutation

Promoter

Available since

May 24, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Beta2-PAmcherry1

Plasmid

#114190

Purpose

studying clustering of Beta2 receptors by superresolution microscopy (PALM)

Depositor

Aleksandra Radenovic

Insert

Adrenergic receptor beta 2 (ADRB2 Human)

Use

Tags

3HA, FLAG, and PAmcherry1

Expression

Mammalian

Mutation

Promoter

SV40

Available since

Oct. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Cerulean-WW-NES

Plasmid

#84629

Purpose

Encodes N-terminal (PAABD) fragment of bimolecular ERK activity reporter (bimEKAR); cytosol targeted

Depositor

Jin Zhang

Article

Depry et al Chem Biol. 2015 Nov 19;22(11):1470-9. do

Insert

Cerulean-WW-NES

Use

Tags

6xHis, Cerulean, Nuclear export signal (NES), T7 …

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+) eGFP PPP1R8 G4

Plasmid

#129021

Purpose

Expresses an eGFP mRNA with the PPP1R8 G-quadruplex (G4) region in the 3' UTR

Depositor

Jeremy Wilusz

Article

Xiao et al Nucleic Acids Res. 2019 Jul 3. pii: 5527

Insert

eGFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+) eGFP EML2 G4

Plasmid

#129023

Purpose

Expresses an eGFP mRNA with the EML2 G-quadruplex (G4) region in the 3' UTR

Depositor

Jeremy Wilusz

Article

Xiao et al Nucleic Acids Res. 2019 Jul 3. pii: 5527

Insert

eGFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3-CARD14-G117S

Plasmid

#69920

Purpose

Expresses mutant FLAG-CARD14 in mammalian cells

Depositor

Francesca Capon

Article

Berki et al J Invest Dermatol. 2015 Jul 23. doi: 10.

Insert

CARD14 isoform 3 (CARD14 Human)

Use

Tags

FLAG

Expression

Bacterial and Mammalian

Mutation

G117S

Promoter

CMV

Available since

Feb. 2, 2016

Availability

Academic Institutions and Nonprofits only
Kohinoor-Paxillin/pcDNA3

Plasmid

#67775

Purpose

Paxilin-targetted Kohinoor for mammalian cell expression

Depositor

Takeharu Nagai

Article

Tiwari et al Nat Methods. 2015 Jun;12(6):515-8. doi:

Insert

Kohinoor

Use

Tags

Paxillin

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 4, 2015

Availability

Academic Institutions and Nonprofits only
pCDNA3.1-hMcl-1(T163A)

Plasmid

#25376

Depositor

Roger Davis

Article

Morel et al Mol Cell Biol. 2009 Jul . 29(14):3845-52

Insert

hMCL1 (MCL1 Human)

Use

Tags

V5 and His tag

Expression

Mammalian

Mutation

changed threonine 163 to alanine

Promoter

Available since

Aug. 23, 2010

Availability

Academic Institutions and Nonprofits only
pY019 (pcDNA3.1-hPmCpf1)

Plasmid

#69991

Purpose

Expresses humanized PmCpf1

Depositor

Feng Zhang

Article

Zetsche et al Cell. 2015 Sep 23. pii: S0092-8674(15)01

Insert

hPmCpf1

Use

CRISPR

Tags

NLS-3xHA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 8, 2015

Availability

Academic Institutions and Nonprofits only
pY009 (pcDNA3.1-hSsCpf1)

Plasmid

#69981

Purpose

Expresses humanized SsCpf1

Depositor

Feng Zhang

Article

Zetsche et al Cell. 2015 Sep 23. pii: S0092-8674(15)01

Insert

hSsCpf1

Use

CRISPR

Tags

NLS-3xHA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 8, 2015

Availability

Academic Institutions and Nonprofits only
pcDNA3-sACE2v1-Fc(IgA1)

Plasmid

#145155

Purpose

Mammalian expression plasmid for soluble ACE2-Fc fusion (IgA1) high affinity variant 1

Depositor

Erik Procko

Article

Chan et al Science. 2020 Sep 4;369(6508):1261-1265.

Insert

Angiotensin-converting enzyme 2 (ACE2 Human)

Use

Tags

Fc region of human IgA1

Expression

Mammalian

Mutation

Extracellular, soluble protease domain (a.a. M1-D…

Promoter

CMV

Available since

April 15, 2020

Availability

Academic Institutions and Nonprofits only

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pCDNA3 14 mut B-Myb

Plasmid

#25967

Depositor

Rob Lewis

Article

Johnson et al J Biol Chem. 2002 Feb 8. 277(6):4088-97.

Insert

B-Myb (Mybl2 Mouse)

Use

Tags

Flag

Expression

Bacterial

Mutation

Changed threonines 267, 408, 443, 447 490, 497, 5…

Promoter

Available since

Sept. 20, 2010

Availability

Academic Institutions and Nonprofits only
pcDNA3.1-deltaA1-VWF

Plasmid

#124823

Purpose

Expresses multimeric human VWF protein without A1 domain. This construct has no tag.

Depositor

Sriram Neelamegham

Article

Zhang et al Blood Adv. 2019 Apr 9;3(7):957-968. doi:

Insert

VWF without A1 domain (VWF Human)

Use

Tags

Expression

Mammalian

Mutation

Deleted A1 domain

Promoter

CMV

Available since

May 10, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(-)-Green Falcan3

Plasmid

#199706

Purpose

green fluorescent indicator for cAMP imaging

Depositor

Tetsuya Kitaguchi

Article

Hiasa et al RSC Adv. 2023 May 22;13(23):15514-15520.

Insert

Green Falcan3

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 24, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Ctdnep1_D67E-2xFlag

Plasmid

#196519

Purpose

Expression of Ctdnep1_D67E-2xFlag

Depositor

Edgar Gomes

Article

Calero-Cuenca et al Curr Biol. 2021 Apr 12;31(7):1521-1530.e

Insert

Ctdnep1_D67E-2xFlag (CTDNEP1 Human)

Use

Tags

2xFlag

Expression

Mammalian

Mutation

D67E mutation (phosphastase dead)

Promoter

CMV promoter; T7 promoter

Available since

March 6, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-C-eGFP-Ctdnep1_D67EsiR

Plasmid

#196518

Purpose

Expression of Ctdnep1_D67E-GFP

Depositor

Edgar Gomes

Article

Calero-Cuenca et al Curr Biol. 2021 Apr 12;31(7):1521-1530.e

Insert

Ctdnep1_D67EsiR (CTDNEP1 Human)

Use

Tags

eGFP

Expression

Mammalian

Mutation

D67E mutation (phosphastase dead). Silent mutatio…

Promoter

CMV promoter; T7 promoter

Available since

March 6, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1+CeGFP-Ctdnep1WTsiR

Plasmid

#196517

Purpose

Expression of Ctdnep1WT-GFP

Depositor

Edgar Gomes

Article

Calero-Cuenca et al Curr Biol. 2021 Apr 12;31(7):1521-1530.e

Insert

Ctdnep1WTsiR (CTDNEP1 Human)

Use

Tags

eGFP

Expression

Mammalian

Mutation

Silent mutations for siRNA resistance

Promoter

CMV promoter; T7 promoter

Available since

Feb. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3-intein/Src

Plasmid

#71406

Purpose

Expresses Src inserted with a Npu DnaE intein (F1 construct)

Depositor

Huiwang Ai

Article

Ren et al J Am Chem Soc. 2015 Feb 18;137(6):2155-8

Insert

Src active kinase domain inserted with Npu DnaE intein

Use

Tags

Expression

Mammalian

Mutation

Promoter

pCMV

Available since

Jan. 27, 2016

Availability

Academic Institutions and Nonprofits only
N-TAP A2A in pcDNA3

Plasmid

#67850

Purpose

mammalian expression of A2A receptor with N terminal TAP tag

Depositor

Christian Gruber

Article

Bergmayr et al J Biol Chem. 2013 Oct 4;288(40):28831-44

Insert

adenosine A2a receptor (ADORA2A Human)

Use

Tags

TAP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 15, 2015

Availability

Academic Institutions and Nonprofits only
FLAG-HA-DRE-1-pcDNA3.1-

Plasmid

#52513

Purpose

expresses C. elegans DRE-1 in mammalian cells with FLAG-HA tag at N-terminus

Depositor

Adam Antebi

Article

Horn et al Dev Cell. 2014 Mar 4. pii: S1534-5807(14

Insert

Dre-1 (dre-1 Nematode)

Use

Tags

FLAG-HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 31, 2015

Availability

Academic Institutions and Nonprofits only

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