We narrowed to 152,744 results for: Des

1. pEF5/FRT-DEST EVC2dW-Flag

   Plasmid

   #41007

   Depositor

   Rajat Rohatgi

   Article

   Dorn et al Dev Cell. 2012 Sep 12. pii: S1534-5807(1

   Insert

   Ellis van Creveld syndrome 2 (EVC2 Mouse)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   deleted amino acids 1177-1220

   Promoter

   Available since

   Nov. 14, 2012

   Availability

   Academic Institutions and Nonprofits only

2. pcDNA 3.2/V5-DEST NET1A S46A

   Plasmid

   #69818

   Purpose

   Expresses NET1A-V5 in mammalian cells

   Depositor

   Anne Brunet

   Article

   Schaffer et al Cell Metab. 2015 Nov 3;22(5):907-21. doi

   Insert

   Neuroepithelial cell-transforming gene 1 protein A (NET1 Human)
   

   Use

   Tags

   V5

   Expression

   Mammalian

   Mutation

   S46A

   Promoter

   CMV

   Available since

   April 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEJS583-pCSDest2-AcrIIC5Smu-FLAG-NLS

   Plasmid

   #113437

   Purpose

   Expresses a FLAG/NLS tagged type II-C anti-CRISPR protein from S. muelleri in mammalian cells

   Depositor

   Erik Sontheimer

   Article

   Lee et al MBio. 2018 Dec 4;9(6). pii: mBio.02321-1

   Insert

   Type II-C anti-CRISPR
   

   Use

   CRISPR

   Tags

   FLAG/NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEF5/FRT/DEST-3xHA/mGli2 P5-6A

   Plasmid

   #51253

   Purpose

   Encodes N-3xHA-TEV-mouseGli2-C; amino acids 923,956 mutated to Ala

   Depositor

   Rajat Rohatgi

   Article

   Niewiadomski et al Cell Rep. 2013 Dec 24. pii: S2211-1247(1

   Insert

   Gli2 (Gli2 Mouse)
   

   Use

   Flpin system

   Tags

   3xHA-TEV

   Expression

   Mammalian

   Mutation

   amino acids 923,956 mutated to Ala

   Promoter

   EF1a

   Available since

   Feb. 25, 2014

   Availability

   Academic Institutions and Nonprofits only

5. pDEST27-GST-ATXN1 FL(84Q)-S776A

   Plasmid

   #21758

   Depositor

   Huda Zoghbi

   Article

   Lim et al Nature. 2008 Apr 10. 452(7188):713-8.

   Insert

   Ataxin-1 (ATXN1 Human)
   

   Use

   Tags

   GST

   Expression

   Mammalian

   Mutation

   insert contains a stretch of 84 Q(Glutamines) and…

   Promoter

   CMV

   Available since

   July 22, 2014

   Availability

   Academic Institutions and Nonprofits only

6. pDEST27-GST-ATXN1 FL(30Q)-S776A

   Plasmid

   #21756

   Depositor

   Huda Zoghbi

   Article

   Lim et al Nature. 2008 Apr 10. 452(7188):713-8.

   Insert

   Ataxin-1 (ATXN1 Human)
   

   Use

   Tags

   GST

   Expression

   Mammalian

   Mutation

   insert contains a stretch of 32 amino acids (30 Q…

   Promoter

   CMV

   Available since

   July 22, 2014

   Availability

   Academic Institutions and Nonprofits only

7. pEF5/FRT-DEST EVC2ala4-Flag

   Plasmid

   #41008

   Depositor

   Rajat Rohatgi

   Article

   Dorn et al Dev Cell. 2012 Sep 12. pii: S1534-5807(1

   Insert

   Ellis van Creveld syndrome 2 (EVC2 Mouse)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   changed amino acids 1183-1186 to Alanine

   Promoter

   Available since

   Nov. 15, 2012

   Availability

   Academic Institutions and Nonprofits only

8. pEF.DEST51-PTPN12(D199A,C231S)-Clover

   Plasmid

   #215528

   Purpose

   Expression vector with a clover-tagged phosphatase-inactive PTP-PEST mutant (D199A,C231S)

   Depositor

   Johanna Ivaska

   Article

   Conway et al Nat Cell Biol. 2025 May 26. doi: 10.1038

   Insert

   PTPN12 (PTPN12 Human)
   

   Use

   Tags

   Clover

   Expression

   Mammalian

   Mutation

   D199A, C231S (Phosphatase inactive)

   Promoter

   EF1a

   Available since

   May 29, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. pLPC-C-SF-TAP-DEST-TLK1- M566T

   Plasmid

   #218831

   Purpose

   Expresses C-terminal Strep-FLAG-tagged M566T human TLK1 variant 5 in a retrovirus vector.

   Depositor

   Travis Stracker

   Insert

   TLK1 M566T (TLK1 Human)
   

   Use

   Tags

   FLAG-STREP

   Expression

   Mammalian

   Mutation

   M566T

   Promoter

   CMV

   Available since

   July 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLPC-C-SF-TAP-DEST-TLK1- Q479E

    Plasmid

    #218830

    Purpose

    Expresses C-terminal Strep-FLAG-tagged Q479E human TLK1 variant 5 in a retrovirus vector.

    Depositor

    Travis Stracker

    Insert

    TLK1 Q479E (TLK1 Human)
    

    Use

    Tags

    FLAG-STREP

    Expression

    Mammalian

    Mutation

    Q479E

    Promoter

    CMV

    Available since

    July 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pDestTol2-QUAS:GFP-CAAX-he1.1:YFP

    Plasmid

    #184816

    Purpose

    Used to generate the Tg(QUAS:GFP-CAAX; he1.1:YFP)c631 transgenic line

    Depositor

    Marnie Halpern

    Article

    Spikol et al Elife. 2024 May 31;12:RP89516. doi: 10.7

    Insert

    QUAS:GFP-CAAX-pA; he1.1:CFP
    

    Use

    Zebrafish tol2 transgenesis

    Tags

    GFP-CAAX

    Expression

    Mutation

    Promoter

    Available since

    May 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDestTol2-QUAS:NLS-mApple-he1.1:CFP

    Plasmid

    #184814

    Purpose

    Used to generate the Tg(QUAS:NLS-mApple; he1.1:CFP)c718 transgenic line

    Depositor

    Marnie Halpern

    Article

    Spikol et al Elife. 2024 May 31;12:RP89516. doi: 10.7

    Insert

    QUAS:NLS-mApple-pA; he1.1:CFP
    

    Use

    Zebrafish tol2 transgenesis

    Tags

    NLS-mApple

    Expression

    Mutation

    Promoter

    Available since

    May 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDestTol2-QUAS:NLS-GFP-he1.1:CFP

    Plasmid

    #184815

    Purpose

    Used to generate the Tg(QUAS:NLS-GFP; he1.1:CFP)c682 transgenic line

    Depositor

    Marnie Halpern

    Article

    Spikol et al Elife. 2024 May 31;12:RP89516. doi: 10.7

    Insert

    QUAS:NLS-GFP-pA; he1.1:CFP
    

    Use

    Zebrafish tol2 transgenesis

    Tags

    NLS-GFP

    Expression

    Mutation

    Promoter

    Available since

    May 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDEST17-His10-Smt3-ccdB-SNAP

    Plasmid

    #166148

    Purpose

    Gateway destination vector for generation of N-terminal His10-Smt3 and C-terminal SNAP tagged proteins in E. coli.

    Depositor

    Daniel Jarosz

    Article

    Harel et al Cell Rep. 2024 Jun 25;43(6):112787. doi:

    Type

    Empty backbone

    Use

    Tags

    His10-Smt3 and SNAP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pDEST17-His10-Smt3-DDX5(kf)-SNAP

    Plasmid

    #166149

    Purpose

    Expression of N. furzeri DDX5 in E. coli

    Depositor

    Daniel Jarosz

    Article

    Harel et al Cell Rep. 2024 Jun 25;43(6):112787. doi:

    Insert

    DDX5
    

    Use

    Tags

    His10-Smt3 and SNAP

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDEST17-His10-Smt3-DDX5ΔPrD(kf)-SNAP

    Plasmid

    #166150

    Purpose

    Expression of N. furzeri DDX5ΔPrD(1-535) mutant in E. coli

    Depositor

    Daniel Jarosz

    Article

    Harel et al Cell Rep. 2024 Jun 25;43(6):112787. doi:

    Insert

    DDX5ΔPrD(1-535)
    

    Use

    Tags

    His10-Smt3 and SNAP

    Expression

    Bacterial

    Mutation

    ΔPrD(1-535)

    Promoter

    T7

    Available since

    May 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDEST17-His10-Smt3-DDX5ΔIDR(kf)-SNAP

    Plasmid

    #166151

    Purpose

    Expression of N. furzeri DDX5ΔIDR(1-483) mutant in E. coli

    Depositor

    Daniel Jarosz

    Article

    Harel et al Cell Rep. 2024 Jun 25;43(6):112787. doi:

    Insert

    DDX5ΔIDR(1-483)
    

    Use

    Tags

    His10-Smt3 and SNAP

    Expression

    Bacterial

    Mutation

    ΔIDR(1-483)

    Promoter

    T7

    Available since

    May 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDN0617 (pDEST-mEGFP C-term, NatR)

    Plasmid

    #210484

    Purpose

    pDEST vector to tag gene of interest with mEGFP on the C-terminus to perform localization assays in S. cerevisiae.

    Depositor

    Christian Landry

    Article

    Evans-Yamamoto et al Mol Biol Evol. 2023 Nov 18:msad246. doi:

    Type

    Empty backbone

    Use

    Synthetic Biology; Destination vector for gateway…

    Tags

    mEGFP

    Expression

    Yeast

    Mutation

    Promoter

    ADH1

    Available since

    Dec. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDEST-Spe4-Kozak-emiRFP703-RfA

    Plasmid

    #186376

    Purpose

    Destination vector for the expression of a fluorescent fusion of N-ter emiRFP703 and a protein of interest into embryos, by mRNA microinjection

    Depositor

    Patrick Lemaire

    Type

    Empty backbone

    Use

    Gateway destination vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. GG-EBNA-MIR302-7guides-PGK-Puro

    Plasmid

    #201960

    Purpose

    EBNA episome plasmid for U6 promoter-driven expression of 7 gRNAs targeting miRNA302/367. Includes PGK-puro selection cassette

    Depositor

    Timo Otonkoski

    Article

    Sokka et al Stem Cell Reports. 2022 Feb 8;17(2):413-

    Insert

    MIR302-7g-PGK-Puro
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    June 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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